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Communication

Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Oncorhynchus mykiss) under Salinity Acclimation

1
Life Science & Technology School, Lingnan Normal University, Zhanjiang 524048, China
2
College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China
3
Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China
*
Author to whom correspondence should be addressed.
Academic Editor: Kenji Saitoh
Animals 2022, 12(10), 1265; https://doi.org/10.3390/ani12101265
Received: 15 March 2022 / Revised: 29 April 2022 / Accepted: 10 May 2022 / Published: 14 May 2022
(This article belongs to the Section Aquatic Animals)
To further understand the molecular mechanism underlying the bone remodeling process, transcriptional information of regenerated scales of rainbow trout under sea water (SW) and fresh water (FW) environments was compared in this study. The miRNA and mRNA expression profiles in regenerated scales were compared to those data collected in ontogenic scales to investigate the effects of salinity acclimation on skeletal growth and development. Gene ontology and KEGG enrichment analysis of the differentially expressed genes and differentially expressed miRNA target genes showed similar expression profiles. These genes were mainly related to ion and energy metabolism.
In order to explore the potential effects of salinity acclimation on bone metabolism of rainbow trout (Oncorhynchus mykiss), transcriptional information of regenerated scales under salinity acclimation (sea water, SW) was compared to those of fish under fresh water (FW) environments. According to the high-throughput sequencing results, a total of 2620 significantly differentially expressed genes (DEGs) were identified in the data of SW vs. FW. Compared with the FW group, six significantly downregulated and 44 significantly upregulated miRNAs were identified in the SW scales (p < 0.05). Furthermore, a total of 994 significantly differentially expressed target genes (DETGs) were identified from the 50 significantly differentially expressed miRNAs (DE miRNAs). Gene ontology analysis of the aforementioned DETGs was similar to the results of the differentially expressed genes (DEGs) obtained from mRNA-seq data, these genes were mainly related to ion metabolism. KEGG enrichment analysis of the DEGs and DETGs suggested that many significantly enriched pathways were related to the energy metabolism pathway. View Full-Text
Keywords: salmonid species; hypersalinity; ncRNA; omics; exoskeleton salmonid species; hypersalinity; ncRNA; omics; exoskeleton
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MDPI and ACS Style

Yang, C.; Zhou, Q.; Ma, Q.; Wang, L.; Yang, Y.; Chen, G. Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Oncorhynchus mykiss) under Salinity Acclimation. Animals 2022, 12, 1265. https://doi.org/10.3390/ani12101265

AMA Style

Yang C, Zhou Q, Ma Q, Wang L, Yang Y, Chen G. Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Oncorhynchus mykiss) under Salinity Acclimation. Animals. 2022; 12(10):1265. https://doi.org/10.3390/ani12101265

Chicago/Turabian Style

Yang, Changgeng, Qiling Zhou, Qian Ma, Liuyong Wang, Yunsheng Yang, and Gang Chen. 2022. "Differentially Expressed miRNAs and mRNAs in Regenerated Scales of Rainbow Trout (Oncorhynchus mykiss) under Salinity Acclimation" Animals 12, no. 10: 1265. https://doi.org/10.3390/ani12101265

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