Bovine Intelectin 2 Expression as a Biomarker of Paratuberculosis Disease Progression
Center for Animal Biotechnology, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), 33394 Deva, Spain
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24071 León, Spain
Departamento de Sanidad Animal, Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, Grulleros, 24346 León, Spain
Department of Animal Health, NEIKER-Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Parque Científico y Tecnológico de Bizkaia, P812, E-48160 Derio, Spain
Author to whom correspondence should be addressed.
Academic Editor: Giulietta Minozzi
Received: 14 April 2021 / Revised: 2 May 2021 / Accepted: 6 May 2021 / Published: 12 May 2021
The potential of the bovine intelectin 2 as a biomarker of Mycobacterium avium subsp. paratuberculosis infection was investigated using quantitative immunohistochemical analysis of ileocecal valve samples of animals with increasing degrees of lesion severity (focal, multifocal and diffuse histological lesions) and control animals without detected lesions. Significant differences were observed in the mean number of intelectin 2 immunolabelled cells between the three histopathological types and the control. Specifically, the mean number of intelectin 2 labelled cells was indicative of disease progression as the focal group had the highest number of intelectin 2 secreting cells followed by the multifocal, diffuse and control groups indicating that intelectin 2 is a good biomarker for the different stages of Mycobacterium avium subsp. paratuberculosis infection. Quantification of bovine intelectin 2 secreting cells could constitute a good post-mortem tool, complementary to histopathology, to improve detection of Mycobacterium avium subsp. Paratuberculosis infections, especially latent forms of infection.