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Article

Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics

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Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono La Esperanza n° 100, 12400 Segorbe, Spain
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Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 46022 Valencia, Spain
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Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Instituto de Ciencias Biomédicas, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Avenida Seminario s/n, 46113 Moncada, Spain
*
Author to whom correspondence should be addressed.
Academic Editors: Rosa María García-García and Maria Arias Alvarez
Animals 2021, 11(4), 1178; https://doi.org/10.3390/ani11041178
Received: 21 March 2021 / Revised: 7 April 2021 / Accepted: 17 April 2021 / Published: 20 April 2021
(This article belongs to the Special Issue Challenges and New Strategies on Rabbit Breeding)
This study was conducted to evaluate the antibacterial activity of two aminopeptidase inhibitors and chitosan-based nanoparticles in liquid-stored rabbit semen. This study reports that the aminopeptidase inhibitors used to prevent bacterial growth could be used in semen extender as a suitable alternative to antibiotics.
Although great attention is paid to hygiene during semen collection and processing, bacteria are commonly found in the semen of healthy fertile males of different species. As the storage of extended semen might facilitate bacterial growth, extenders are commonly supplemented with antibiotics. This study aimed to evaluate the antibacterial activity of ethylenediaminetetraacetic acid (EDTA), bestatin and chitosan-based nanoparticles added to rabbit semen extender and their effect on reproductive performance under field conditions. Four different extenders were tested, supplemented with antibiotics (TCG+AB), with EDTA and bestatin (EB), with EDTA, bestatin and chitosan-based nanoparticles (QEB) or without antibiotics (TCG-AB). Extended semen was cooled at 15 °C for three days. Cooled samples were examined for bacterial growth and semen quality every 24 h for 3 days. The enterobacteria count increased considerably during storage at 72 h in semen extended with TCG+AB and TCG-AB, while extenders EB and QEB showed a bacteriostatic effect over time. After 24, 48 and 72 h, quality characteristics were retained in all groups, with no significant motility differences, either in acrosome integrity, membrane functionality or the viability of spermatozoa. Additionally, bacterial concentration present in fresh semen did not affect reproductive performance. In conclusion, EDTA and bestatin exerted a potent bacteriostatic effect over time and could be used as an alternative to conventional antibiotics in rabbit semen extenders. View Full-Text
Keywords: artificial insemination; antibiotics; antibacterial activity; semen quality; reproductive performance artificial insemination; antibiotics; antibacterial activity; semen quality; reproductive performance
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MDPI and ACS Style

Viudes-de-Castro, M.P.; Marco-Jimenez, F.; Vicente, J.S.; Marin, C. Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics. Animals 2021, 11, 1178. https://doi.org/10.3390/ani11041178

AMA Style

Viudes-de-Castro MP, Marco-Jimenez F, Vicente JS, Marin C. Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics. Animals. 2021; 11(4):1178. https://doi.org/10.3390/ani11041178

Chicago/Turabian Style

Viudes-de-Castro, María P., Francisco Marco-Jimenez, José S. Vicente, and Clara Marin. 2021. "Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics" Animals 11, no. 4: 1178. https://doi.org/10.3390/ani11041178

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