A Z-Linked E3 Ubiquitin Ligase Cs-rchy1 Is Involved in Gametogenesis in Chinese Tongue Sole, Cynoglossus semilaevis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Approval
2.2. Fish and Tissue Collection
2.3. Sequence Analysis and Alignment
2.4. cDNA Synthesis and Quantitative Real-Time PCR (qPCR) Analysis
2.5. Cellular Localization of Cs-rchy1 mRNA in Gonads
2.6. siRNA-Mediated Interference of Cs-rchy1 in Gonadal Cell Lines
3. Results
3.1. Cloning and Characteristics of CS-rchy1
3.2. Tissue Expression Patterns of Cs-rchy1
3.3. Expression Profile of Cs-rchy1 at Different Developmental Stages of Gonads
3.4. Localization of Cs-rchy1 mRNA in Gonads
3.5. In Vitro RNAi-Mediated Cs-rchy1 Knockdown and Its Influence on the Expression of Sex-Related Genes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Sequences (5′–3′) | Purpose | Product Size |
---|---|---|---|
rchy1 probeF | CACCGCGGCCGCAGCAATGGGAGCAGATAG | ISH | 213 bp |
rchy1 probeR | CACCCTCGAGGCTGATGTTGTGCGTGAA | ||
rchy1 F | TTTACACAAGACCTGCTTTG | qPCR | 108 bp |
rchy1 R | TCATCTATCTGCTCCCATT | ||
figla_tv1 F | ACATAGAGAAGTTCAAACGAGCC | qPCR | 210 bp |
figla_tv1 R | CGGTAGCAGCTTTTAGTGTGTCT | ||
sox9 F | AAGAACCACACAGATCAAGACAGA | qPCR | 150 bp |
sox9 R | TAGTCATACTGTGCTCTGGTGATG | ||
sox-9-A F | GACCAAGTGTGTAATGTGACCAAG | qPCR | 227 bp |
sox-9-A R | GCTCTTGGTGTTGTTATATCCACG | ||
cyp19a F | GGTGAGGATGTGACCCAGTGT | qPCR | 230 bp |
cyp19a R | ACGGGCTGAAATCGCAAG | ||
igf1 F | GTATCTCCTGTAGCCACACCCTCT | qPCR | 137 bp |
igf1 R | GCCTCTCTCTCCACACACAAACT | ||
β-actin F | CCTTGGTATGGAGTCCTGTGGC | qPCR | 150 bp |
β-actin R | TCCTTCTGCATCCTGTCGGC | ||
scaffold68-2F | CCTAAATGATGGATGTAGATTCTGTC | Sex genotype | |
scaffold68-2R | GATCCAGAGAAAATAAACCCAGG |
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Sun, Y.; Zhu, Y.; Cheng, P.; Zhang, M.; Wang, N.; Cui, Z.; Wei, M.; Xu, W. A Z-Linked E3 Ubiquitin Ligase Cs-rchy1 Is Involved in Gametogenesis in Chinese Tongue Sole, Cynoglossus semilaevis. Animals 2021, 11, 3265. https://doi.org/10.3390/ani11113265
Sun Y, Zhu Y, Cheng P, Zhang M, Wang N, Cui Z, Wei M, Xu W. A Z-Linked E3 Ubiquitin Ligase Cs-rchy1 Is Involved in Gametogenesis in Chinese Tongue Sole, Cynoglossus semilaevis. Animals. 2021; 11(11):3265. https://doi.org/10.3390/ani11113265
Chicago/Turabian StyleSun, Yuxuan, Ying Zhu, Peng Cheng, Mengqian Zhang, Na Wang, Zhongkai Cui, Min Wei, and Wenteng Xu. 2021. "A Z-Linked E3 Ubiquitin Ligase Cs-rchy1 Is Involved in Gametogenesis in Chinese Tongue Sole, Cynoglossus semilaevis" Animals 11, no. 11: 3265. https://doi.org/10.3390/ani11113265