Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Ethics
2.2. Tissue Collection
2.3. Cell Culture
2.4. GnRH Analogue Treatment for Cows
2.5. GnRH Analogue Treatment for Rats
2.6. GnRH Treatment for Cells
2.7. Transfection
2.8. RNA Extraction, Reverse Transcription, and Real-Time q-PCR
2.9. Dual Luciferase Reporter Assay
2.10. ELISA
2.11. Apoptosis Analysis
2.12. MiRNA Target Prediction
2.13. Statistical Analysis
3. Results
3.1. GnRH Analogue Treatment Promoted the Synthesis and Secretion of FSH and LH in Cows
3.2. MiR-488 Targeted the FSHB 3’UTR
3.3. Low-Frequency GnRH Analogue Treatment Promoted the Synthesis and Secretion of FSH and LH in Rats
3.4. Regulation of FSH Synthesis by Differentially Expressed miR-488 in Rat Anterior Adenohypophyseal Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Primer Name | Sequence (5’–3’) | |
---|---|---|---|
Rat | Gapdh | Forward primer | GGAAACCCATCACCATCTTC |
Reverse primer | GTGGTTCACACCCATCACAA | ||
Fshβ | Forward primer | ATACCACTTGGTGTGAGGGC | |
Reverse primer | TAGAGGGAGTCTGAGTGGCG | ||
Lhβ | Forward primer | CAAAAGCCAGGTCAGGGATA | |
Reverse primer | GTACTCGAACCATGCTAGGACA | ||
U6 | Forward primer | GCTTCGGCAGCACATATACTAAAAT | |
Reverse primer | CGCTTCACGAATTTGCGTGTCAT | ||
U6 | Reverse transcription | CGCTTCACGAATTTGCGTGTCAT | |
miR-488 | Reverse transcription | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTGAG | |
miR-488 | Forward primer | ACACTCCAGCTGGGGCCTTCCGGT | |
universal reverse | CTCAAGTGTCGTGGAGTCGGCAA |
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Wang, H.-Q.; Wang, W.-H.; Chen, C.-Z.; Guo, H.-X.; Jiang, H.; Yuan, B.; Zhang, J.-B. Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells. Animals 2021, 11, 3262. https://doi.org/10.3390/ani11113262
Wang H-Q, Wang W-H, Chen C-Z, Guo H-X, Jiang H, Yuan B, Zhang J-B. Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells. Animals. 2021; 11(11):3262. https://doi.org/10.3390/ani11113262
Chicago/Turabian StyleWang, Hao-Qi, Wen-Hua Wang, Cheng-Zhen Chen, Hai-Xiang Guo, Hao Jiang, Bao Yuan, and Jia-Bao Zhang. 2021. "Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells" Animals 11, no. 11: 3262. https://doi.org/10.3390/ani11113262