A Preliminary Study on the Relationship between Parasitaemia and Cytokine Expression of Peripheral Blood Cells in Trypanosoma vivax-Experimentally Infected Cattle
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Infection and Sample Collection
2.2. qPCR (Quantitative Real-Time PCR) for Trypanosoma vivax Detection and Quantification
2.3. RNA Extraction and cDNA Transcription
2.4. Cytokine Assessment by RT-qPCR
3. Results
3.1. qPCR for Trypanosoma vivax
3.2. Cytokine Assessment by RT-qPCR
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cytokine | Primers (5′-3′) | GenBank Access Number |
---|---|---|
IL-1β | F- CAAGGAGAGGAAAGAGACA R- TGAGAAGTGCTGATGTACCA | M37211 |
IL-2 | F- TTTTACGTGCCCAAGGTTAA R- CGTTTACTGTTGCATCATCA | M12791 |
IL-4 | F- CAAAGAACACAACTGAGAAG R- AGGTCTTTCAGCGTACTTGT | M77120 |
IL-6 | F- TCCAGAACGAGTATGAGG R- CATCCGAATAGCTCTCAG | X57317 |
IL-10 | F- TGCTGGATGACTTTAAGGG R- AGGGCAGAAAGCGATGACA | U00799 |
IL-12 p40 | F- AACCTGCAACTGAGACCATT R- ATCCTTGTGGCATGTGACTT | U11815 |
IFNγ | F- ATAACCAGGTCATTCAAAGG R- ATTCTGACTTCTCTTCCGCT | M29867 |
TNFα | F- CCAGAGGGAAGAGCAGTCC R- GGCTACAACGTGGGCTACC | NM_173966 |
gapdh | F- TCGGAGTGAACGGATTCG R- ATCTCGCTCCTGGAAGATG | NM_001034034 |
β-actin | F- CGCACCACTGGCATTGTCAT R- TCCAAGGCGACGTAGCAGAG | K00622 |
H3F3A | F- GAGGTCTCTATACCATGGCTC R- GTACCAGGCCTGTAACGATG | NM_00101489 |
YWHAZ | F- GAAAGGGATTGTGGACCAG R- GGCTTCATCAAATGCTGTCT | NM_174814 |
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Fidelis Junior, O.L.; Sampaio, P.H.; Gonçalves, L.R.; Machado, R.Z.; André, M.R.; Wijffels, G.; Cadioli, F.A. A Preliminary Study on the Relationship between Parasitaemia and Cytokine Expression of Peripheral Blood Cells in Trypanosoma vivax-Experimentally Infected Cattle. Animals 2021, 11, 3191. https://doi.org/10.3390/ani11113191
Fidelis Junior OL, Sampaio PH, Gonçalves LR, Machado RZ, André MR, Wijffels G, Cadioli FA. A Preliminary Study on the Relationship between Parasitaemia and Cytokine Expression of Peripheral Blood Cells in Trypanosoma vivax-Experimentally Infected Cattle. Animals. 2021; 11(11):3191. https://doi.org/10.3390/ani11113191
Chicago/Turabian StyleFidelis Junior, Otavio Luiz, Paulo Henrique Sampaio, Luiz Ricardo Gonçalves, Rosangela Zacarias Machado, Marcos Rogério André, Gene Wijffels, and Fabiano Antonio Cadioli. 2021. "A Preliminary Study on the Relationship between Parasitaemia and Cytokine Expression of Peripheral Blood Cells in Trypanosoma vivax-Experimentally Infected Cattle" Animals 11, no. 11: 3191. https://doi.org/10.3390/ani11113191