Next Article in Journal
Veterinarians’ Knowledge, Attitudes and Practices Associated with Bovine Viral Diarrhoea Virus Control and Prevention in South-East Australia
Next Article in Special Issue
Seawater Culture Increases Omega-3 Long-Chain Polyunsaturated Fatty Acids (N-3 LC-PUFA) Levels in Japanese Sea Bass (Lateolabrax japonicus), Probably by Upregulating Elovl5
Previous Article in Journal
Semen Quality of Rasa Aragonesa Rams Carrying the FecXR Allele of the BMP15 Gene
Open AccessBrief Report

Immune Responses of Asian Seabass Lates calcarifer to Dietary Glycyrrhiza uralensis

by 1,2,3, 1,3, 1,3, 1,3, 1,2,3, 1,2 and 1,2,3,*
1
Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China
2
Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
3
Sanya Tropical Fisheries Research Institute, Sanya 572018, China
*
Author to whom correspondence should be addressed.
Animals 2020, 10(9), 1629; https://doi.org/10.3390/ani10091629
Received: 1 August 2020 / Revised: 26 August 2020 / Accepted: 9 September 2020 / Published: 11 September 2020
Due to the fact of their low toxicity, small side effects, and little residue, increasing attention has been paid to herbs as environmentally friendly immunostimulants. Results from the present study indicate that adding Glycyrrhiza uralensis to the feed can improve the growth and survival of Lates calcarifer and significantly promote the expression of immune-related genes in the liver and head kidney of Lates calcarifer. The optimum inclusion level of Glycyrrhiza uralensis should be 1–3%.
To understand the impacts of dietary Glycyrrhiza uralensis on the immune responses of Lates calcarifer, the expression of immune-related genes including crp, c-3, c-4, mtor, mlst-8, eif4e, hsp-70, hsp-90, il-8il-8, il-10, tgfβ1, tnf, ifn-γ1, and mxf in L. calcarifer juveniles was evaluated in this study. Fish were fed experimental diets with G. uralensis levels of 0%, 1%, 3%, and 5% for 56 days. The results showed that dietary G. uralensis could improve the growth and survival of L. calcarifer and regulate the immune-related genes’ expression in L. calcarifer. Dietary G. uralensis significantly upregulated the expression level of crp, mtor, hsp-90, c-3, and c-4 genes in the liver of L. calcarifer, while hsp-70 gene expression was nearly downregulated. It did not upregulate the expression of elf4e and mlst-8 in the 1% and 3% inclusion groups, but it was the exact opposite in the 5% inclusion group. G. uralensis significantly affected the expression of il-8, il-10, tnf, ifn-γ1, mxf, and tgfβ1 in the head kidney of L. calcarifer. G. uralensis upregulated the expression of tnf and tgfβ1 consistently, but ifn-γ1 was at a low expression level. The expression of il-8 and il-10 was upregulated in the 1% group, while it was downregulated in the 5% group. The results from the present study indicate that dietary G. uralensis appeared to improve the immune function of L. calcarifer, and the optimum inclusion level should be between 1–3%. View Full-Text
Keywords: Lates calcarifer; Glycyrrhiza uralensis; immune-related genes; gene expression Lates calcarifer; Glycyrrhiza uralensis; immune-related genes; gene expression
Show Figures

Figure 1

MDPI and ACS Style

Yang, R.; Han, M.; Fu, Z.; Wang, Y.; Zhao, W.; Yu, G.; Ma, Z. Immune Responses of Asian Seabass Lates calcarifer to Dietary Glycyrrhiza uralensis. Animals 2020, 10, 1629. https://doi.org/10.3390/ani10091629

AMA Style

Yang R, Han M, Fu Z, Wang Y, Zhao W, Yu G, Ma Z. Immune Responses of Asian Seabass Lates calcarifer to Dietary Glycyrrhiza uralensis. Animals. 2020; 10(9):1629. https://doi.org/10.3390/ani10091629

Chicago/Turabian Style

Yang, Rui; Han, Mingyang; Fu, Zhengyi; Wang, Yifu; Zhao, Wang; Yu, Gang; Ma, Zhenhua. 2020. "Immune Responses of Asian Seabass Lates calcarifer to Dietary Glycyrrhiza uralensis" Animals 10, no. 9: 1629. https://doi.org/10.3390/ani10091629

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop