Characterization of microRNAs during Embryonic Skeletal Muscle Development in the Shan Ma Duck
College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
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Author to whom correspondence should be addressed.
Animals 2020, 10(8), 1417; https://doi.org/10.3390/ani10081417
Received: 15 July 2020
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Revised: 12 August 2020
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Accepted: 13 August 2020
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Published: 14 August 2020
(This article belongs to the Collection Genomics, Transcriptomics, and Computational Biology for Biodiversity Studies and Quality-Related Traits Selection in Livestock)
Simple Summary
It is of great commercial interest to elucidate the genetic mechanisms associated with skeletal muscle development in the duck. In this study, we performed high throughput microRNA (miRNA) sequencing to identify the candidate miRNAs during two developmental stages of duck embryonic breast muscle. We detected 1091 miRNAs and 109 of them were differentially expressed between embryonic day 13 (E13) and E19. We also predicted the target genes of the differentially expressed miRNAs and subsequently analyzed the enriched gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways, and finally constructed a protein–protein interaction (PPI) network with the target genes. Luciferase reporter assay showed that the growth-related genes, Fibroblast growth factor receptor like 1 (FGFRL1) and Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1), were target genes of miR-212-5p. These results can supplement the duck miRNA database and provide several candidate miRNAs for future studies on the regulation of embryonic skeletal muscle development.
Poultry skeletal muscle provides high quality protein for humans. Study of the genetic mechanisms during duck skeletal muscle development contribute to future duck breeding and meat production. In the current study, three breast muscle samples from Shan Ma ducks at embryonic day 13 (E13) and E19 were collected, respectively. We detected microRNA (miRNA) expression using high throughput sequencing following bioinformatic analysis. qRT-PCR validated the reliability of sequencing results. We also identified target prediction results using the luciferase reporter assay. A total of 812 known miRNAs and 279 novel miRNAs were detected in six samples; as a result, 61 up-regulated and 48 down-regulated differentially expressed miRNAs were identified between E13 and E19 (|log2 fold change| ≥ 1 and p ≤ 0.05). Enrichment analysis showed that target genes of the differentially expressed miRNAs were enriched on many muscle development-related gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, especially mitogen-activated protein kinase (MAPK) signaling pathways. An interaction network was constructed using the target genes of the differentially expressed miRNAs. These results complement the current duck miRNA database and offer several miRNA candidates for future studies of skeletal muscle development in the duck.
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MDPI and ACS Style
Li, C.; Xiong, T.; Zhou, M.; Wan, L.; Xi, S.; Liu, Q.; Chen, Y.; Mao, H.; Liu, S.; Chen, B. Characterization of microRNAs during Embryonic Skeletal Muscle Development in the Shan Ma Duck. Animals 2020, 10, 1417. https://doi.org/10.3390/ani10081417
AMA Style
Li C, Xiong T, Zhou M, Wan L, Xi S, Liu Q, Chen Y, Mao H, Liu S, Chen B. Characterization of microRNAs during Embryonic Skeletal Muscle Development in the Shan Ma Duck. Animals. 2020; 10(8):1417. https://doi.org/10.3390/ani10081417
Chicago/Turabian StyleLi, Chuan, Ting Xiong, Mingfang Zhou, Lei Wan, Suwang Xi, Qiuhong Liu, Yi Chen, Huirong Mao, Sanfeng Liu, and Biao Chen. 2020. "Characterization of microRNAs during Embryonic Skeletal Muscle Development in the Shan Ma Duck" Animals 10, no. 8: 1417. https://doi.org/10.3390/ani10081417
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