Investigation of the Impacts of Antibiotic Exposure on the Diversity of the Gut Microbiota in Chicks
by
, , ,
Abdelmotaleb A. Elokil
1,2
,
Khaled F.M. Abouelezz
3,
Hafiz I. Ahmad
4,
Yuanhu Pan
1 and
Shijun Li
1,* 1
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2
Department of Animal Production, Faculty of Agriculture, Benha University, Moshtohor 13736, Egypt
3
Department of Poultry Production, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt
4
Department of Livestock Production, University of Veterinary and Animal sciences, Ravi Campus, Pattoki 55300, Pakistan
*
Author to whom correspondence should be addressed.
Animals 2020, 10(5), 896; https://doi.org/10.3390/ani10050896
Submission received: 24 April 2020
/
Revised: 15 May 2020
/
Accepted: 18 May 2020
/
Published: 21 May 2020
(This article belongs to the Special Issue Gut Health in Poultry)
Simple Summary
Broad-spectrum antibiotics have been a cornerstone in the treatment of bacterial diseases. However, growing evidence suggests that antibiotics have effects on host-associated gut microbiota communities. In this study, we report persistent significant changes in the abundance of gut microbiota and their functional metabolite pathways in chickens due to enrofloxacin and diclazuril exposure. These changes may affect the taxonomic, genomic, and functional capacity of the chicken gut microbiota, reducing bacterial diversity while expanding and collapsing membership of specific indigenous taxa. Understanding the biology of competitive exclusion of adaptive functions during antibiotic exposure in the gut may inform the design of new strategies to treat infections, while preserving the ecology of chicken-beneficial constituents.
Abstract
The dynamic microbiota in chickens can be affected by exposure to antibiotics, which may alter the composition and substrate availability of functional pathways. Here, 120 Jing Hong chicks at 30 days of age were randomly divided into four treatments totaling seven experimental groups: control chicks not exposed to antibiotics; and chicks exposed to enrofloxacin, diclazuril, and their mixture at 1:1 for 14 days and then not exposed for a withdrawal period of 15 days. Fecal samples were collected from the 7 groups at 8 time-points (exposure to 4 antibiotics and 4 withdrawal periods) to perform in-depth 16S rRNA sequencing of the gut microbiota. Taxon-independent analysis showed that the groups had significantly distinct microbial compositions (p < 0.01). Based on the microbial composition, as compared with the control group, the abundances of the phyla Firmicutes, Actinobacteria, Thermi, and Verrucomicrobia, as well as the families Lactobacillus, Lactococcus, S24-7, and Corynebacterium, were decreased in the antibiotic-exposed chicks (p < 0.01). Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analyses revealed significant differences in microbiota metabolite pathways due to the genera of the antibiotic-responsive microbes (p < 0.01), especially the pathways relating to cell growth and death, immune system diseases, carbohydrate metabolism, and nucleotide metabolism. Oral treatment with enrofloxacin, diclazuril, and their mixture modified the gut microbiota composition and the microbial metabolic profiles in chickens, with persistent effects (during the withdrawal period) that prevented the return to the original community and led to the formation of a new community.
Keywords:
enrofloxacin; diclazuril; gut microbiome; metagenomics; chicken
