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Article

Comparison of Selenium Source in Preventing Oxidative Stress in Bovine Mammary Epithelial Cells

1
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Department of Dairy and Animal Science, Penn State University, 324 Henning Bldg., University Park, Jamba, PA 16801, USA
3
CAAS-ICRAF Joint Lab on Agroforestry and Sustainable Animal Husbandry, Beijing 100193, China
4
Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, China
*
Author to whom correspondence should be addressed.
Animals 2020, 10(5), 842; https://doi.org/10.3390/ani10050842
Received: 12 April 2020 / Revised: 8 May 2020 / Accepted: 9 May 2020 / Published: 13 May 2020
(This article belongs to the Special Issue Sustainable Animal Nutrition and Feeding)
Selenium (Se) is recognized as an essential trace element in maintaining antioxidant status in humans and animals. Se supplementation in the diets of livestock exist in two forms: Organic and inorganic forms. The organic Se source hydroxy-selenomethionine (HMSeBA) has been proven to be more biologically efficient than inorganic Se to improve antioxidant capacity when fed to dairy cows, since its approval as a feed additive by the European Commission in 2013. However, information on the comparison between HMSeBA and other Se sources in preventing oxidative stress in bovine mammary epithelial cells (BMEC) is limited. The current study compared the effects of HMSeBA, selenomethionine (SeMet) and sodium selenite (SS) on antioxidant capacity and the ability to resist oxidative stress induced by H2O2 in BMEC. HMSeBA was shown to enhance cellular antioxidant status to resist oxidative damage when compared with SS, but there was no difference between HMSeBA and SeMet. The results of this study provide more information for antioxidant potential of different Se sources in BMEC.
Oxidative stress can cause cell damage. Hydroxy-selenomethionine (HMSeBA) is an organic Se source with emerging antioxidant advantages. The objective of this study was to compare the effects of HMSeBA, selenomethionine (SeMet) and sodium selenite (SS) on the antioxidant response and the ability to resist oxidative stress in bovine mammary epithelial cells (BMEC). The BMEC were treated with 0 (Control), 20, 50, 100 and 150 nM HMSeBA, 100 nM SeMet and100 nM SS for 48 h. The results showed that HMSeBA and SeMet treatments had higher glutathione peroxidase (p < 0.01) and catalase (p = 0.01) activities and mRNA abundance of GPX3 (p = 0.02), but lower superoxide dismutase activity compared with SS (p = 0.04). The catalase activity (p < 0.05) and mRNA abundance of GPX3 (p = 0.04) changed in a quadratic manner with the increase of HMSeBA levels. To assess the potential protection of different Se sources against oxidative stress on BMEC, 0 or 50 μM H2O2 was added to BMEC culture for 3 h after Se pre-treatment for 48 h. The results showed that HMSeBA and SeMet, which did not differ (p > 0.05), but further decreased malondialdehyde and reactive oxygen species production compared with SS (p < 0.05). In conclusion, HMSeBA showed an enhanced cellular antioxidant status to resist oxidative damage induced by H2O2 when compared with SS, whereas the effects were similar to SeMet. View Full-Text
Keywords: bovine mammary epithelial cell; hydroxy-selenomethionine; antioxidant capacity; oxidative stress bovine mammary epithelial cell; hydroxy-selenomethionine; antioxidant capacity; oxidative stress
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MDPI and ACS Style

Sun, L.; Wang, F.; Wu, Z.; Ma, L.; Baumrucker, C.; Bu, D. Comparison of Selenium Source in Preventing Oxidative Stress in Bovine Mammary Epithelial Cells. Animals 2020, 10, 842. https://doi.org/10.3390/ani10050842

AMA Style

Sun L, Wang F, Wu Z, Ma L, Baumrucker C, Bu D. Comparison of Selenium Source in Preventing Oxidative Stress in Bovine Mammary Epithelial Cells. Animals. 2020; 10(5):842. https://doi.org/10.3390/ani10050842

Chicago/Turabian Style

Sun, Lingling, Fang Wang, Zhaohai Wu, Lu Ma, Craig Baumrucker, and Dengpan Bu. 2020. "Comparison of Selenium Source in Preventing Oxidative Stress in Bovine Mammary Epithelial Cells" Animals 10, no. 5: 842. https://doi.org/10.3390/ani10050842

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