In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review
1
Department of Agriculture, Forestry, and Food Sciences, University of Turin, 10095 Grugliasco, Italy
2
Institute of Sciences of Food Production, National Research Council, 10095 Grugliasco, Italy
*
Author to whom correspondence should be addressed.
Animals 2020, 10(5), 775; https://doi.org/10.3390/ani10050775
Received: 13 April 2020
/
Revised: 22 April 2020
/
Accepted: 22 April 2020
/
Published: 29 April 2020
(This article belongs to the Special Issue In Vitro Digestibility in Animal Nutritional Studies)
Simple Summary
The Ankom DaisyII incubator (ADII; Ankom Technology Corporation Fairport, NY, USA) has gained acceptance as an alternative to traditional in vitro procedures. It reduces the labour requirement and increases the number of determinations that can be completed by a single operator. The apparatus allows for the simultaneous incubation of several feedstuffs in sealed polyester bags in the same incubation vessel, which is rotated continuously at 39.5 °C. With this method, the material that disappears from the bag during incubation is considered digestible. The method, which was first developed to predict the digestibility of feedstuffs for ruminants, has been modified and adapted to improve its accuracy and prediction capacity. Modifications used by various researchers include the use of different inocula, buffer solutions, and sample weights. Recently, attempts have been made to adapt the method to determine nutrient digestibility of feedstuff in non-ruminant animals, including pets.
This review summarises the use of the Ankom DaisyII incubator (ADII; Ankom Technology Corporation Fairport, NY, USA), as presented in studies on digestibility, and its extension to other species apart from ruminants, from its introduction until today. This technique has been modified and adapted to allow for different types of investigations to be conducted. Researchers have studied and tested different procedures, and the main sources of variation have been found to be: the inoculum source, sample size, sample preparation, and bag type. In vitro digestibility methods, applied to the ADII incubator, have been reviewed, the precision and accuracy of the method using the ADII incubator have been dealt with, and comparisons with other methods have been made. Moreover, some hypotheses on the possible evolutions of this technology in non-ruminants, including pets, have been described. To date, there are no standardised protocols for the collection, storage, and transportation of rumen fluid or faeces. There is also still a need to standardise the procedures for washing the bags after digestion. Moreover, some performance metrics of the instrument (such as the reliability of the rotation mechanism of the jars) still require improvement.
View Full-Text
Keywords:
in vitro digestibility; inoculum; rumen fluid; faeces; enzyme; Ankom DaisyII incubator
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Supplementary File 1:
PDF-Document (PDF, 173 KiB)
MDPI and ACS Style
Tassone, S.; Fortina, R.; Peiretti, P.G. In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review. Animals 2020, 10, 775. https://doi.org/10.3390/ani10050775
AMA Style
Tassone S, Fortina R, Peiretti PG. In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review. Animals. 2020; 10(5):775. https://doi.org/10.3390/ani10050775
Chicago/Turabian StyleTassone, Sonia, Riccardo Fortina, and Pier G. Peiretti. 2020. "In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review" Animals 10, no. 5: 775. https://doi.org/10.3390/ani10050775
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
