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Article

Changes in Serum and Salivary Proteins in Canine Mammary Tumors

1
Interdisciplinary Laboratory of Clinical Pathology, Interlab-UMU, University of Murcia, 30100 Murcia, Spain
2
Mediterranean Institute for Life Sciences (MedILS), 21000 Split, Croatia
3
Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia
4
Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 30 Gleboka St., 20-612 Lublin, Poland
*
Author to whom correspondence should be addressed.
Animals 2020, 10(4), 741; https://doi.org/10.3390/ani10040741
Received: 27 March 2020 / Revised: 17 April 2020 / Accepted: 21 April 2020 / Published: 24 April 2020
(This article belongs to the Special Issue Biomarkers in Animal Health and Disease)
The present study describes for the first time the differences in the serum and saliva proteomes between healthy bitches and bitches with mammary tumors using a high-throughput proteomic approach. More than 1000 proteins were identified and 35 in serum and 49 in saliva were significantly modulated. Additionally, their related pathways were discussed in order to improve understanding of the pathophysiology of the disease and one protein, serum albumin, was further validated. The results of the present study could be a source of potential biomarkers for canine mammary tumors in saliva and serum and increase the knowledge on the pathophysiology of the disease.
The aim of this study was to evaluate changes in serum and saliva proteomes in canine mammary tumors (CMT) using a high-throughput quantitative proteomic analysis in order to potentially discover possible biomarkers of this disease. Proteomes of paired serum and saliva samples from healthy controls (HC group, n = 5) and bitches with CMT (CMT group, n = 5) were analysed using a Tandem Mass Tags-based approach. Twenty-five dogs were used to validate serum albumin as a candidate biomarker in an independent sample set. The proteomic analysis quantified 379 and 730 proteins in serum and saliva, respectively. Of those, 35 proteins in serum and 49 in saliva were differentially represented. The verification of albumin in serum was in concordance with the proteomic data, showing lower levels in CMT when compared to the HC group. Some of the modulated proteins found in the present study such as haptoglobin or S100A4 have been related to CMT or human breast cancer previously, while others such as kallikrein-1 and immunoglobulin gamma-heavy chains A and D are described here for the first time. Our results indicate that saliva and serum proteomes can reflect physiopathological changes that occur in CMT in dogs and can be a potential source of biomarkers of the disease. View Full-Text
Keywords: biomarker; cancer; canine mammary tumors; dog; proteomics; saliva; serum biomarker; cancer; canine mammary tumors; dog; proteomics; saliva; serum
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MDPI and ACS Style

Franco-Martínez, L.; Gelemanović, A.; Horvatić, A.; Contreras-Aguilar, M.D.; Dąbrowski, R.; Mrljak, V.; Cerón, J.J.; Martínez-Subiela, S.; Tvarijonaviciute, A. Changes in Serum and Salivary Proteins in Canine Mammary Tumors. Animals 2020, 10, 741. https://doi.org/10.3390/ani10040741

AMA Style

Franco-Martínez L, Gelemanović A, Horvatić A, Contreras-Aguilar MD, Dąbrowski R, Mrljak V, Cerón JJ, Martínez-Subiela S, Tvarijonaviciute A. Changes in Serum and Salivary Proteins in Canine Mammary Tumors. Animals. 2020; 10(4):741. https://doi.org/10.3390/ani10040741

Chicago/Turabian Style

Franco-Martínez, Lorena, Andrea Gelemanović, Anita Horvatić, María D. Contreras-Aguilar, Roman Dąbrowski, Vladimir Mrljak, José J. Cerón, Silvia Martínez-Subiela, and Asta Tvarijonaviciute. 2020. "Changes in Serum and Salivary Proteins in Canine Mammary Tumors" Animals 10, no. 4: 741. https://doi.org/10.3390/ani10040741

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