Mutations in NLRP5 and NLRP9 Are Associated with Litter Size in Small Tail Han Sheep
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Preparation and Sample Collection
2.2. Genotyping
2.3. Statistical Analysis
2.4. Protein Interaction Networks Predicted by STRING Database
3. Results
3.1. Population Genetic Analysis of SNPs in NLRP5 and NLRP9
3.2. Associations between Five Loci in NLRP5 and NLRP9 with Litter Size in Small Tail Han Sheep
3.3. Predicted Protein Interaction Networks Involving NLRP5 and NLRP9
4. Discussion
4.1. NLRP5 and NLRP9 Polymorphisms
4.2. Association Analyses of NLRP5 and NLRP9
4.3. Reproductive Functions of GDF9 and Its Interactions with NLRP5 and NLRP9
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Breed | Number | Type | District |
---|---|---|---|
Small Tail Han sheep | 384 | Year-round Breeding | Southwest region, Shandong Province, China |
Hu sheep | 83 | Year-round Breeding | Xuzhou, Jiangsu Province, China |
Cele Black sheep | 68 | Year-round Breeding | Cele, Xinjiang Uygur Autonomous Region, China |
Sunite sheep | 70 | Seasonal Breeding | Wulatezhongqi, Bayannaoer, Inner Mongolia Autonomous Region, China |
Prairie Tibetan sheep | 80 | Seasonal Breeding | Dangxiong, Tibet Autonomous Region, China |
Suffolk sheep | 60 | Seasonal Breeding | Beijing Aoxin Stud Farm Co. Ltd. located in Shunyi District, Beijing, China |
Tan sheep | 23 | Seasonal Breeding | Yanchi, Ningxia Hui Autonomous Region, China |
Locus | Breed | Genotype Frequency | Allele Frequency | PIC | HE | NE | Chi-Square Test (p-Value) | |||
---|---|---|---|---|---|---|---|---|---|---|
g.60495375 A > G | AA | AG | GG | A | G | |||||
Prairie Tibetan sheep | 0.61 | 0.25 | 0.14 | 0.74 | 0.26 | 0.31 | 0.39 | 1.63 | 0.00 | |
Cele Black sheep | 0.62 | 0.34 | 0.04 | 0.79 | 0.21 | 0.28 | 0.34 | 1.50 | 0.95 | |
Hu sheep | 0.52 | 0.35 | 0.13 | 0.69 | 0.31 | 0.34 | 0.43 | 1.74 | 0.10 | |
Suffolk sheep | 0.75 | 0.20 | 0.05 | 0.85 | 0.15 | 0.23 | 0.26 | 1.35 | 0.10 | |
Sunite sheep | 0.54 | 0.37 | 0.09 | 0.73 | 0.27 | 0.32 | 0.40 | 1.65 | 0.61 | |
Tan sheep | 0.87 | 0.09 | 0.04 | 0.91 | 0.09 | 0.15 | 0.16 | 1.19 | 0.03 | |
Small Tail Han Sheep | 0.74 | 0.24 | 0.02 | 0.86 | 0.14 | 0.21 | 0.24 | 1.32 | 0.87 | |
g.60495363 G > A | AA | AG | GG | A | G | |||||
Prairie Tibetan sheep | 0.62 | 0.35 | 0.03 | 0.80 | 0.20 | 0.27 | 0.32 | 1.47 | 0.40 | |
Cele Black sheep | 0.46 | 0.46 | 0.08 | 0.68 | 0.32 | 0.34 | 0.43 | 1.76 | 0.65 | |
Hu sheep | 0.47 | 0.42 | 0.11 | 0.68 | 0.32 | 0.34 | 0.43 | 1.77 | 0.79 | |
Suffolk sheep | 0.26 | 0.52 | 0.22 | 0.52 | 0.48 | 0.37 | 0.50 | 2.00 | 0.78 | |
Sunite sheep | 0.60 | 0.36 | 0.04 | 0.78 | 0.22 | 0.29 | 0.34 | 1.53 | 0.76 | |
Tan sheep | 0.65 | 0.26 | 0.09 | 0.78 | 0.22 | 0.28 | 0.34 | 1.52 | 0.26 | |
Small Tail Han Sheep | 0.45 | 0.43 | 0.12 | 0.66 | 0.34 | 0.35 | 0.45 | 1.81 | 0.52 | |
g.60499690 C > A | CC | CA | AA | C | A | |||||
Prairie Tibetan sheep | 0.39 | 0.46 | 0.15 | 0.62 | 0.38 | 0.36 | 0.47 | 1.89 | 0.86 | |
Cele Black sheep | 0.29 | 0.56 | 0.15 | 0.57 | 0.43 | 0.37 | 0.49 | 1.96 | 0.24 | |
Hu sheep | 0.22 | 0.46 | 0.32 | 0.45 | 0.55 | 0.37 | 0.49 | 1.98 | 0.50 | |
Suffolk | 0.53 | 0.42 | 0.05 | 0.73 | 0.27 | 0.31 | 0.39 | 1.64 | 0.40 | |
Sunite sheep | 0.40 | 0.44 | 0.16 | 0.62 | 0.38 | 0.36 | 0.47 | 1.89 | 0.62 | |
Tan sheep | 0.57 | 0.39 | 0.04 | 0.76 | 0.24 | 0.30 | 0.36 | 1.57 | 0.72 | |
Small Tail Han Sheep | 0.47 | 0.41 | 0.12 | 0.67 | 0.33 | 0.34 | 0.44 | 1.78 | 0.22 |
Locus | Breed | Genotype Frequency | Allele Frequency | PIC | HE | NE | Chi-Square Test (p-Value) | |||
---|---|---|---|---|---|---|---|---|---|---|
g.59030623 T > C | TT | TC | CC | T | C | |||||
Prairie Tibetan sheep | 0.81 | 0.18 | 0.01 | 0.90 | 0.10 | 0.16 | 0.18 | 1.22 | 0.80 | |
Cele Black sheep | 0.91 | 0.09 | 0.00 | 0.96 | 0.04 | 0.08 | 0.08 | 1.09 | 0.70 | |
Hu sheep | 0.75 | 0.25 | 0.00 | 0.87 | 0.13 | 0.20 | 0.22 | 1.28 | 0.19 | |
Suffolk | 0.52 | 0.38 | 0.10 | 0.71 | 0.29 | 0.33 | 0.41 | 1.70 | 0.58 | |
Sunite sheep | 0.63 | 0.37 | 0.00 | 0.81 | 0.19 | 0.26 | 0.30 | 1.43 | 0.06 | |
Tan sheep | 0.83 | 0.17 | 0.00 | 0.91 | 0.09 | 0.15 | 0.16 | 1.19 | 0.65 | |
Small Tail Han Sheep | 0.74 | 0.23 | 0.03 | 0.85 | 0.15 | 0.22 | 0.25 | 1.34 | 0.07 | |
g.59043397 A > C | AA | CA | CC | A | C | |||||
Prairie Tibetan sheep | 0.86 | 0.13 | 0.01 | 0.92 | 0.08 | 0.13 | 0.14 | 1.16 | 0.38 | |
Cele Black sheep | 0.56 | 0.37 | 0.07 | 0.74 | 0.26 | 0.31 | 0.38 | 1.62 | 0.75 | |
Hu sheep | 0.48 | 0.36 | 0.16 | 0.66 | 0.34 | 0.35 | 0.45 | 1.81 | 0.08 | |
Suffolk | 0.95 | 0.05 | 0.00 | 0.98 | 0.02 | 0.05 | 0.05 | 1.05 | 0.84 | |
Sunite sheep | 0.83 | 0.16 | 0.01 | 0.91 | 0.09 | 0.15 | 0.17 | 1.20 | 0.57 | |
Tan sheep | 0.65 | 0.35 | 0.00 | 0.83 | 0.17 | 0.25 | 0.29 | 1.40 | 0.31 | |
Small Tail Han Sheep | 0.78 | 0.20 | 0.02 | 0.88 | 0.12 | 0.19 | 0.22 | 1.28 | 0.05 |
Locus | Genotype | Litter Size | ||
---|---|---|---|---|
First Parity (N) | Second Parity (N) | Third Parity (N) | ||
g.60495375 A > G | AA | 1.88 ± 0.039(283) | 2.12 ± 0.058(170) | 2.40 ± 0.101(68) |
AG | 1.83 ± 0.069(92) | 2.11 ± 0.103(54) | 2.53 ± 0.191(19) | |
GG | 2.00 ± 0.234(8) | 2.40 ± 0.340(5) | 3.00 ± 0.416(4) | |
g.60495363 G > A | AA | 1.81 ± 0.051(169) b | 2.06 ± 0.077(97) b | 2.37 ± 0.133(38) b |
GA | 1.89 ± 0.052(164) a,b | 2.07 ± 0.078(94) b | 2.33 ± 0.137(36) b | |
GG | 2.12 ± 0.098(46) a | 2.37 ± 0.128(35) a | 2.86 ± 0.205(16) a | |
g.60499690 C > A | CC | 1.86 ± 0.049(180) | 2.10 ± 0.073(107) | 2.49 ± 0.137(37) |
CA | 1.85 ± 0.053(158) | 2.07 ± 0.077(98) | 2.36 ± 0.137(42) | |
AA | 2.00 ± 0.098(46) | 2.10 ± 0.073(24) | 2.67 ± 0.241(12) |
Locus | Genotype | Litter Size | ||
---|---|---|---|---|
First Parity (N) | Second Parity (N) | Third Parity (N) | ||
g.59030623 T > C | TT | 1.85 ± 0.039(283) b | 2.10 ± 0.062(152) b | 2.34 ± 0.120(47) b |
TC | 1.88 ± 0.071(88) b | 2.13 ± 0.095(64) b | 2.46 ± 0.139(35) b | |
CC | 2.25 ± 0.200(11) a | 2.55 ± 0.229(11) a | 3.00 ± 0.273(9) a | |
g.59043397 A > C | AA | 1.87 ± 0.038(298) | 2.10 ± 0.056(186) | 2.47 ± 0.097(75) |
AC | 1.85 ± 0.077(75) | 2.18 ± 0.124(38) | 2.27 ± 0.223(14) | |
CC | 2.20 ± 0.210(10) | 2.25 ± 0.381(4) | 3.00 ± 0.591(2) |
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Zhang, Z.; Tang, J.; He, X.; Di, R.; Chu, M. Mutations in NLRP5 and NLRP9 Are Associated with Litter Size in Small Tail Han Sheep. Animals 2020, 10, 689. https://doi.org/10.3390/ani10040689
Zhang Z, Tang J, He X, Di R, Chu M. Mutations in NLRP5 and NLRP9 Are Associated with Litter Size in Small Tail Han Sheep. Animals. 2020; 10(4):689. https://doi.org/10.3390/ani10040689
Chicago/Turabian StyleZhang, Zhuangbiao, Jishun Tang, Xiaoyun He, Ran Di, and Mingxing Chu. 2020. "Mutations in NLRP5 and NLRP9 Are Associated with Litter Size in Small Tail Han Sheep" Animals 10, no. 4: 689. https://doi.org/10.3390/ani10040689