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Article

L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method

1
University Centre of Veterinary Medicine UJ-UR, University of Agriculture in Krakow, 30-059 Krakow, Poland
2
Artvimed Centre for Reproductive Medicine, 30-147 Krakow, Poland
3
Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, 30-083 Balice/Krakow, Poland
*
Author to whom correspondence should be addressed.
Animals 2020, 10(4), 658; https://doi.org/10.3390/ani10040658
Received: 25 March 2020 / Revised: 6 April 2020 / Accepted: 7 April 2020 / Published: 10 April 2020
(This article belongs to the Special Issue Reproductive Management of Sheep and Goats)
The laparoscopic method of recovering oocytes in goats and sheep is one of the minimally invasive methods used in the biotechnology of animal reproduction. It allows for good quality oocytes that are suitable for in vitro maturation and fertilization to be recovered. The limitation of using the laparoscopic ovum pick-up (L-OPU) method in goat and sheep is its changing effectiveness and the lack of repeatability of results, as well as the varying effectiveness of different variants of the method. Therefore, it is necessary to develop effective non-invasive techniques allowing for multiple good quality oocyte recovery that would be suitable for in vitro maturation and fertilization. In this study, four different L-OPU variants were described in goats and sheep. Various techniques of recovering oocytes were discussed, including the techniques of conducting the operation, various tools for recovering oocytes, and different plans of hormonal stimulation. Recovery rates were 35% (Variant I), 57% (Variant II), 72% (Variant III), and 67% (Variant IV). After evaluation, 94% (both Variant I and II), 93% (Variant III), and 84% (Variant IV) of the oocytes were qualified for in vitro maturation. The results of the study show that the proposed technique of laparoscopic recovery of oocytes allows a sufficient number of ovarian cells suitable for in vitro culture to be obtained and as a consequence it makes them useful in in vitro maturation/in vitro fertilization (IVM/IVF) programs or cloning. The method allows for a fast and effective conduct of the operation in a living donor with minimal invasiveness while preserving the excellent condition of animals. View Full-Text
Keywords: laparoscopy; ovum pick up; oocyte; goat; sheep laparoscopy; ovum pick up; oocyte; goat; sheep
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MDPI and ACS Style

Wieczorek, J.; Koseniuk, J.; Skrzyszowska, M.; Cegła, M. L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method. Animals 2020, 10, 658. https://doi.org/10.3390/ani10040658

AMA Style

Wieczorek J, Koseniuk J, Skrzyszowska M, Cegła M. L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method. Animals. 2020; 10(4):658. https://doi.org/10.3390/ani10040658

Chicago/Turabian Style

Wieczorek, Jarosław; Koseniuk, Jurij; Skrzyszowska, Maria; Cegła, Mirosław. 2020. "L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method" Animals 10, no. 4: 658. https://doi.org/10.3390/ani10040658

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