L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method
Abstract
Simple summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Procedure
2.3. Statistical Analyses
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Variant I (Goats) | Variant II (Goats) | Variant III (Goats) | Variants I to III in Total (Goats) | Variant IV (Goats) | Sheep | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. | A | B | No. | A | B | No. | A | B | No. | A | B | No. | A | B | No. | A | B | ||
Number of ovarian follicles | 98 | 66 | 246 | 410 | 202 | 212 | |||||||||||||
Number of aspirated follicles | 96 | 65 | 238 | 399 | 195 | 205 | |||||||||||||
Total number of recovered oocytes | 34 | 35.4 * ± 0.2 | 37 | 56.9 ± 0.3 | 139 | 72.1 * ± 0.2 | 210 | 52.6 ± 0.3 | 132 | 67.7 ± 0.2 | 137 | 66.8 ± 0.3 | |||||||
Not qualified for IVM (Class IV) | 2 | 2.1 ± 0.04 | 5.9 ± 0.1 | 2 | 3.1 ±0.07 | 5.4 ± 0.1 | 10 | 4.2 ± 0.1 | 7.2 ± 0.3 | 14 | 3.5 ± 0.1 | 6.7 ± 0.2 | 20 | 10.3 * ± 0.1 | 15.2 * ± 0.2 | 19 | 9.3 * ± 0.2 | 13.9 * ± 0.4 | |
Qualified for IVM Total (1 + 2 + 3) | 32 | 33.3 * ± 0.2 | 94 ± 0.1 | 35 | 53.8 ± 0.3 | 94.6 ± 0.1 | 129 | 54.2 ± 0.3 | 92.8 ± 0.3 | 196 | 49.1 ± 0.3 | 93.3 ± 0.2 | 112 | 57.4 ± 0.2 | 84.8 ± 0.2 | 118 | 57.6 ± 0.3 | 86.1 ± 0.3 | |
Morphology oocytes qualified for IVM | Class I (1) | 12 | 12.5 * ± 1.4 | 35.3 * ± 0.4 | 11 | 16.9 ± 0.2 | 29.7 ± 0.3 | 55 | 23.1 ± 0.3 | 39.6 * ± 0.4 | 78 | 19.5 ± 0.3 | 37.1 * ± 0.4 | 58 | 29.7 ± 0.2 | 43.9 * ± 0.3 | 44 | 21.5 ± 0.3 | 32.1 ± 0.4 |
Class II (2) | 17 | 17.7 ± 1.9 | 50.0 * ± 0.4 | 18 | 27.7 ± 2.0 | 48.6 * ± 0.4 | 52 | 21.8 ± 0.3 | 37.4 ± 0.4 | 87 | 21.8 ± 0.3 | 41.4* ± 0.4 | 16 | 8.2 * ± 0.2 | 12.1 * ± 0.3 | 58 | 28.3 ± 0.3 | 42.3 ± 0.4 | |
Class III (3) | 3 | 3.1 ± 0.1 | 8.8 ± 0.2 | 6 | 9.2 ± 0.2 | 16.2 * ± 0.9 | 22 | 9.2 ± 0.1 | 15.8 ± 0.3 | 31 | 7.8 ± 0.1 | 14.8 ± 0.3 | 38 | 19.5 ± 0.2 | 28.8 * ± 0.2 | 16 | 7.8 ± 0.05 | 11.7 ± 0.08 |
Variant I (Goat) | Variant II (Goat) | Variant III (Goat) | Variant I to III in Total(Goat) | Variant IV (Goat) | Sheep | |||
---|---|---|---|---|---|---|---|---|
Number of ovarian follicles | Mean ± SD | 9.8 * ± 4.2 | 6.6 ± 3.5 | 5.7 ± 5.4 | 6.5 ± 5.1 | 8.1 ± 4.1 | 4.7 ± 2.4 | |
Min | 5 | 4 | 0 | 0 | 2 | 1 | ||
Max | 20 | 11 | 25 | 25 | 21 | 11 | ||
Number of aspirated follicles | Mean ± SD | 9.6 * ± 4.3 | 6.5 * ± 3.4 | 5.5 ± 5.4 | 6.3 ± 5.1 | 7.8 ± 4.3 | 4.6 ± 2.3 | |
Min | 4 | 1 | 0 | 0 | 2 | 0 | ||
Max | 20 | 9 | 25 | 25 | 21 | 11 | ||
Total number of recovered oocytes (%) | Mean ± SD | 3.4 ± 1.2 | 3.7 ± 2.4 | 3.2 ± 3.4 | 3.3 ± 3.0 | 5.3 * ± 3.6 | 3.0 ± 1.8 | |
Min | 2 | 0 | 0 | 0 | 2 | 0 | ||
Max | 5 | 9 | 18 | 18 | 17 | 9 | ||
Not qualified for IVM (Class IV) | Mean ± SD | 0.2 ± 0.41 | 0.2 ± 0.44 | 0.2 ± 0.5 | 0.2 ± 0.5 | 0.2 ± 0.4 | 0.4 ± 0.6 | |
Min | 0 | 0 | 0 | 0 | 0 | |||
Max | 1 | 1 | 2 | 2 | 2 | |||
Qualified for IVM Total (1 + 2 + 3) | Mean ± SD | 3.2 ± 1.2 | 3.5 ± 2.4 | 3.0 ± 3.3 | 3.1 ± 2.9 | 4.5 * ± 1.7 | 2.62 ± 1.9 | |
Min | 2 | 0 | 0 | 0 | 2 | 0 | ||
Max | 5 | 9 | 17 | 17 | 6 | 9 | ||
Morphology oocytes qualified for IVM | Class I (1) | Mean ± SD | 1.2 ± 1.3 | 1.1 ± 2.2 | 1.3 ± 2.0 | 1.2 ± 1.9 | 2.3 * ± 1.2 | 1.1 ± 1.3 |
Min | 0 | 0 | 0 | 0 | 0 | 0 | ||
Max | 3 | 7 | 7 | 7 | 4 | 4 | ||
Class II (2) | Mean ± SD | 1.7 ± 1.9 | 1.8 ± 1.6 | 1.2 ± 2.3 | 1.4 ± 2.1 | 0.6 ± 1.1 | 1.29 ± 1.7 | |
Min | 0 | 0 | 0 | 0 | 0 | 0 | ||
Max | 5 | 5 | 13 | 13 | 3 | 7 | ||
Class III (3) | Mean ± SD | 0.3 ± 0.5 | 0.6 ± 0.8 | 0.5 ± 0.9 | 0.5 ± 0.8 | 1.52 * ± 1.14 | 0.36 ± 0.9 | |
Min | 0 | 0 | 0 | 0 | 0 | 0 | ||
Max | 1 | 2 | 2 | 2 | 3 | 4 |
Method | Method Effectiveness (%) | Oocytes Qualified for In Vitro Maturation * | Author/Date |
---|---|---|---|
L-OPU ** | 71.8 | Graff 1998 [15] | |
TUGA *** | 61 | ||
L-OPU | 87.3 | Baldassarre 2002 [38] | |
82.1 | |||
73 | |||
83.7 | |||
L-OPU | 85.7 | 92.5 | Baldassarre 2003 [1] |
L-OPU | 69.3 | 93.6 | Cogniė 2003 [16] |
56.9 | 28.5 | ||
L-OPU | 56.25 | 46.4 | Koeman 2003 [31] |
88.8 | 50.7 | ||
L-OPU | 87.6 | 72 | Baldassarre 2007 [39] |
L-OPU | 81.2 | Cox 2007 [17] | |
L-OPU | 74.3 | 84 | Souza 2013 [40] |
L-OPU | 47.4–86.5 | Cordeiro 2014 [27] | |
L-OPU | 91.2 | 85.1 | Nor 2014 [41] |
Post slaughter | 73.0 | 57.2 | Islam 2007 [42] |
Method | Method Effectiveness (%) | Oocytes Qualified for in Vitro Maturation * | Author/Date |
---|---|---|---|
L-OPU | 79.5 | 60 | Baldassarre 1996 [19] |
77.3 | 57 | ||
L-OPU | 67.9 | 83 | Kühholzer 1997 [26] |
L-OPU | 55.5 | 65–70 | Stangl 1999 [14] |
L-OPU | 56.2 | 85.7 | Morton 2005 [43] |
38.8 | 81.8 | ||
L-OPU | 28.3–69.5 | Rodríguez 2006 [20] | |
L-OPU | 85.6 | Cox 2007 [17] | |
L-OPU | 51.7 | Teixeira 2011[28] | |
L-OPU ** | 63.8 | 86.1 | Wieczorek 2018 [13] |
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Wieczorek, J.; Koseniuk, J.; Skrzyszowska, M.; Cegła, M. L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method. Animals 2020, 10, 658. https://doi.org/10.3390/ani10040658
Wieczorek J, Koseniuk J, Skrzyszowska M, Cegła M. L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method. Animals. 2020; 10(4):658. https://doi.org/10.3390/ani10040658
Chicago/Turabian StyleWieczorek, Jarosław, Jurij Koseniuk, Maria Skrzyszowska, and Mirosław Cegła. 2020. "L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method" Animals 10, no. 4: 658. https://doi.org/10.3390/ani10040658
APA StyleWieczorek, J., Koseniuk, J., Skrzyszowska, M., & Cegła, M. (2020). L-OPU in Goat and Sheep—Different Variants of the Oocyte Recovery Method. Animals, 10(4), 658. https://doi.org/10.3390/ani10040658