Effects of Single Nucleotide Polymorphisms in the SLC27A3 Gene on the Nutritional Value of Sheep Milk
1
Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, Poland
2
Department of Genetics, Faculty of Biotechnology and Animal Breeding, West Pomeranian University of Technology in Szczecin, Piastów Avenue 45, 79-311 Szczecin, Poland
3
Department of Ruminant Science, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, ul. Klemensa Janickiego 29, Szczecin, 71-270 Szczecin, Poland
4
Institute of Animal Breeding Wroclaw University of Environmental and Life Sciences, ul. Chelmonskiego 38C, 51-631 Wroclaw, Poland
*
Author to whom correspondence should be addressed.
Animals 2020, 10(4), 562; https://doi.org/10.3390/ani10040562
Received: 26 February 2020
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Revised: 24 March 2020
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Accepted: 24 March 2020
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Published: 27 March 2020
(This article belongs to the Special Issue Biomarkers Involved in Meat and Milk Yield, Composition and Technological Properties in Livestock Species)
Simple Summary
The aim of the present study was to identify single nucleotide polymorphisms (SNPs) in the SLC27A3 gene in sheep and to analyse correlations between selected genotypes and the nutritional value of milk. Milk and dairy products are basic elements of the human diet, due to their components having functional properties. Genetic potential of animals should be used to obtain food with the best technological and nutritional parameters. Results from the presented study suggest that milk from sheep with TT genotype at SNP4 is characterised by good technological and nutritional value. A high content of unsaturated fatty acids is observed in milk from sheep with GG in SNP1 and with CC in SNP3 of the SLC27A3 gene.
The current research was undertaken to use the genetic potential of animals to obtain high-quality dairy products. Single nucleotide polymorphisms (SNPs) in SLC27A3 gene were identified in Zošľachtená valaška sheep using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Correlations between genotypes and milk composition and nutritional value were analysed This study showed that milk from sheep with TT genotype in the SNP4 locus was characterised by higher (p < 0.01) fat and dry matter content and lower lactose concentration, compared to sheep with AA and TA genotypes, respectively. Moreover, it was found that animals with GG genotype in SNP1 produced milk with higher C18:1n9c, C18:1n7t, CLA, and other unsaturated fatty acids (UFAs) content than sheep with TT. Additionally, milk from animals with CC at the SNP3 locus had significantly higher (p < 0.01) levels of UFAs than milk from sheep with other genotypes in the SNP3. In summary, it may be concluded that milk from animals with TT genotype of SNP4 is characterised by higher fat and dry matter content. Whereas, milk from sheep with GG in SNP1 and with CC in SNP3 is characterised by higher content of UFAs, which increases milk value as material for functional food production.
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Keywords:
sheep; milk; fatty acids; SNPs
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MDPI and ACS Style
Pecka-Kiełb, E.; Kowalewska-Łuczak, I.; Czerniawska-Piątkowska, E.; Zielak-Steciwko, A.E. Effects of Single Nucleotide Polymorphisms in the SLC27A3 Gene on the Nutritional Value of Sheep Milk. Animals 2020, 10, 562. https://doi.org/10.3390/ani10040562
AMA Style
Pecka-Kiełb E, Kowalewska-Łuczak I, Czerniawska-Piątkowska E, Zielak-Steciwko AE. Effects of Single Nucleotide Polymorphisms in the SLC27A3 Gene on the Nutritional Value of Sheep Milk. Animals. 2020; 10(4):562. https://doi.org/10.3390/ani10040562
Chicago/Turabian StylePecka-Kiełb, Ewa, Inga Kowalewska-Łuczak, Ewa Czerniawska-Piątkowska, and Anna E. Zielak-Steciwko. 2020. "Effects of Single Nucleotide Polymorphisms in the SLC27A3 Gene on the Nutritional Value of Sheep Milk" Animals 10, no. 4: 562. https://doi.org/10.3390/ani10040562
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