Proteomic Analysis of Fresh and Liquid-Stored Boar Spermatozoa
Institute of Preclinical Sciences, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
Clinic for Reproduction and Large Animals, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
Department of Health Sciences, University ‘Magna Græcia´of Catanzaro, Viale Europa, 88100 Catanzaro, Italy
Department of Veterinary Medicine, Università degli Studi di Milano, Via dell‘Università 6, 26900 Lodi, Italy
Department of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, Via della Commenda 10, 20122 Milano, Italy
Author to whom correspondence should be addressed.
Received: 14 February 2020 / Revised: 19 March 2020 / Accepted: 23 March 2020 / Published: 26 March 2020
Male gametes can be stored for a long period of time for the purpose of preserving genetic material. Cryopreservation and liquid preservation are two main storage procedures commonly used for boar semen. There is evidence in the literature suggesting that cryopreservation changes the profile of proteins that are linked to the motility and function of spermatozoa. It was postulated that they can affect motility, capacitation, oocyte binding ability, and the acrosome reaction of spermatozoa. On the other hand, little is known about changes in protein levels in sperm that occur during liquid storage. Therefore, the objective of this study was to investigate whether liquid storage also causes an alteration in the proteomic profile of stored spermatozoa. A comparative proteomic approach was used to analyze protein samples from fresh spermatozoa and spermatozoa stored for three days at 15–17 °C. Results obtained show that liquid preservation causes quantitative changes in the boar sperm proteome with the over-expression of three out of four proteins in the liquid-stored sperm. Our findings can help elucidate the events involved in liquid preservation.