Metformin Improves Quality of Post-Thaw Canine Semen
INRAE UMR85 Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, France
CNRS UMR7247 Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, France
Université de Tours, 37000 Tours, France
IFCE, 37380 Nouzilly, France
Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 49, 50-366 Wroclaw, Poland
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 20 January 2020 / Revised: 7 February 2020 / Accepted: 7 February 2020 / Published: 12 February 2020
Cryopreservation of semen is getting easier, however, fertilizing results after insemination with frozen-thawed semen is still not constant in canine species depending on the breed and could be still improved. In this study, we decided to modulate the mitochondrial activity through the addition of metformin in semen extender to increase germ cells’ quality. Metformin presented the absence of toxicity and an improvement in sperm motility after thawing, as well as an increase in the expression of several molecular markers associated with quality. In addition, the oxidative stress and DNA damage were reduced in semen frozen in the presence of metformin. Overall, these data suggest that metformin added in canine semen extender has beneficial effects on canine semen quality and could be associated with different components such as vitamins, to enhance the antioxidants status.