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Open AccessCommunication

Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples

1
Departamento de Inspeção e Produtos de Origem Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte 31270-901, Brazil
2
Departamento de Clínica Médica, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil
3
Programa de Pós-Graduação em Ciência Animal, Universidade Federal da Paraíba, Areia 58397-000, Brazil
4
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil
5
Curso de Medicina Veterinária, Universidade Santo Amaro, Rua Prof. Enéas de Siqueira Neto 340, São Paulo 04829-300, Brazil
*
Author to whom correspondence should be addressed.
Animals 2020, 10(11), 2143; https://doi.org/10.3390/ani10112143
Received: 4 September 2020 / Revised: 13 October 2020 / Accepted: 14 October 2020 / Published: 18 November 2020
(This article belongs to the Special Issue The Role of Microorganism in Bovine Mastitis)
Staphylococcus aureus is a major, prevalent mastitis pathogen, representing a real issue for bovine udder health, with unquestionable importance in human and veterinary medicine. The present study thus aimed to determine the antimicrobial resistance and the diversity of S. aureus recovered from transient and persistent intramammary infections and from extramammary niches (e.g., nares/muzzles) in dairy cows. We found that a large proportion of S. aureus strains exhibited multidrug resistance to antimicrobials, including resistance to antimicrobials that are critically important to human health. S. aureus isolates from transient and persistent IMIs did not differ, suggesting that the persistence of bovine intramammary infections (IMIs) was mainly determined by host factors, although S. aureus isolated from extramammary niches are not an important source of S. aureus intramammary infections. Furthermore, a discrepancy in antimicrobial resistance between S. aureus strains isolated from nares/muzzles and intramammary infections was observed.
In the present study, we aimed to determine the antimicrobial resistance and molecular typing of Staphylococcus aureus recovered from transient and persistent intramammary infections and nares/muzzles in dairy cows. We investigated the antimicrobial resistance of 189 S. aureus strains using a broad antimicrobial susceptibility profile. Furthermore, 107 S. aureus isolates were strain-typed using staphylococcal protein-A (spa) typing. A large proportion of strains exhibited multidrug resistance to antimicrobials, including resistance to critically important antimicrobials, although no methicillin-resistant S. aureus strains were found. Our study did not strengthen the idea that extramammary niches (i.e., nares/muzzles) are an important source of S. aureus for bovine mastitis. A discrepancy in the antimicrobial resistance between S. aureus strains isolated from nares/muzzles and milk samples was observed. Furthermore, S. aureus isolates from transient and persistent intramammary infections (IMIs) did not differ by spa typing, suggesting that the persistence of bovine IMIs was determined by cow factors. Thus, the high level of multidrug-resistant S. aureus found in the two herds, considered together with the predominance of a well udder-adapted S. aureus strain, may contribute to our knowledge of the history of the high prevalence of mastitis caused by S. aureus, which is of great concern for animal and public health. View Full-Text
Keywords: intramammary infection; spa typing; staphylococci; antimicrobial susceptibility; dairy cow intramammary infection; spa typing; staphylococci; antimicrobial susceptibility; dairy cow
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    Doi: 10.5281/zenodo.4014439
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    Description: Table S1. Antimicrobial susceptibility profile of the Staphylococcus aureus isolates from milk and nasal/muzzles swabs samples performed by the automated Vitek 2® compact system by minimum inhibitory concentration.
MDPI and ACS Style

Santos, R.P.; Souza, F.N.; Oliveira, A.C.D.; de Souza Filho, A.F.; Aizawa, J.; Moreno, L.Z.; da Cunha, A.F.; Cortez, A.; Della Libera, A.M.M.P.; Heinemann, M.B.; Cerqueira, M.M.O.P. Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples. Animals 2020, 10, 2143. https://doi.org/10.3390/ani10112143

AMA Style

Santos RP, Souza FN, Oliveira ACD, de Souza Filho AF, Aizawa J, Moreno LZ, da Cunha AF, Cortez A, Della Libera AMMP, Heinemann MB, Cerqueira MMOP. Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples. Animals. 2020; 10(11):2143. https://doi.org/10.3390/ani10112143

Chicago/Turabian Style

Santos, Renata P.; Souza, Fernando N.; Oliveira, Ana C.D.; de Souza Filho, Antônio F.; Aizawa, Juliana; Moreno, Luisa Z.; da Cunha, Adriano F.; Cortez, Adriana; Della Libera, Alice M.M.P.; Heinemann, Marcos B.; Cerqueira, Mônica M.O.P. 2020. "Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples" Animals 10, no. 11: 2143. https://doi.org/10.3390/ani10112143

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