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cAMP Response Element Binding Protein 1 (CREB1) Promotes Monounsaturated Fatty Acid Synthesis and Triacylglycerol Accumulation in Goat Mammary Epithelial Cells

by 1,†, 1,†, 1, 1, 2, 1,* and 3,*
1
Tianjin Institute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China
2
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, Shaanxi, China
3
Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA
*
Authors to whom correspondence should be addressed.
Dawei Yao and Chunlei Yang contributed equally to this paper.
Animals 2020, 10(10), 1871; https://doi.org/10.3390/ani10101871
Received: 16 September 2020 / Revised: 2 October 2020 / Accepted: 7 October 2020 / Published: 14 October 2020
(This article belongs to the Special Issue Animal Genetics and Livestock Production: The Biodiversity Challenge)
In non-ruminant liver and adipose tissue, cAMP response element binding protein 1(CREB1) is essential for lipid synthesis and triacylglycerol accumulation. The present study aimed to ascertain the role of CREB1 in regulating milk fatty acid composition synthesized by goat mammary gland. Our data found that overexpression of CREB1 in vitro alters the abundance of lipogenic genes, triacylglycerol accumulation and concentration of monounsaturated fatty acids in goat mammary epithelial cells. Thus, manipulation of CREB1 in vivo might be one approach to improve the quality of goat milk.
cAMP response element binding protein 1 (CREB1) is a member of the leucine zipper transcription factor family of DNA binding proteins. Although studies in non-ruminants have demonstrated a crucial role of CREB1 in lipid synthesis in liver and adipose tissue, it is unknown if this transcription regulator exerts control of fatty acid synthesis in ruminant mammary cells. To address this question, we first defined the expression dynamics of CREB1 in mammary tissue during lactation. Analysis of CREB1 in mammary tissue revealed higher mRNA abundance in mammary tissue harvested at peak lactation. Overexpression of CREB1 markedly upregulated sterol regulatory element binding transcription factor 1 (SREBP1), fatty acid synthase (FASN), acetyl-coenzyme A carboxylase α (ACACA), elongase of very long chain fatty acids 6 (ELOVL6), lipoprotein lipase (LPL), fatty acid binding protein 3 (FABP3), lipin 1 (LPIN1) and diacylglycerol acyltransferase 1 (DGAT1), but had no effect on glycerol-3-phosphate acyltransferase, mitochondrial (GPAM) or 1-acylglycerol-3-phosphate O-acyltransferase 6 (AGPAT6). In addition, overexpressing CREB1 led to a significant increase in the concentration and desaturation index of C16:1 (palmitoleic acid) and C18:1 (oleic acid), along with increased concentration of triacylglycerol. Taken together, these results highlight an important role of CREB1 in regulating lipid synthesis in goat mammary epithelial cells. Thus, manipulation of CREB1 in vivo might be one approach to improve the quality of goat milk. View Full-Text
Keywords: fatty acid composition; monounsaturated fatty acids; goat milk; dairy nutrition fatty acid composition; monounsaturated fatty acids; goat milk; dairy nutrition
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Yao, D.; Yang, C.; Ma, J.; Chen, L.; Luo, J.; Ma, Y.; Loor, J.J. cAMP Response Element Binding Protein 1 (CREB1) Promotes Monounsaturated Fatty Acid Synthesis and Triacylglycerol Accumulation in Goat Mammary Epithelial Cells. Animals 2020, 10, 1871.

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