In Vitro Evaluation of the Effects of Tylosin on the Composition and Metabolism of Canine Fecal Microbiota
Department of Veterinary Medical Sciences, University of Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia (BO), Italy
Department for Life Quality Studies, University of Bologna, Corso d’Augusto 237, 47921 Rimini, Italy
Author to whom correspondence should be addressed.
Received: 31 October 2019 / Revised: 20 December 2019 / Accepted: 3 January 2020 / Published: 8 January 2020
The antibiotic-responsive enteropathy is a common canine chronic disorder for which tylosin represents an effective widely used therapeutic option, although its mechanism of action, beyond the well-known antibacterial activity, is still unclear. Given the beneficial role of prebiotic substrates for gut health, positive outcomes deriving from the association of tylosin with some prebiotic oligosaccharides might be supposed. The present study investigated in vitro the effects of tylosin, alone or supplemented with fructooligosaccharides, galactooligosaccharides, or xylooligosaccharides, on the composition and activity of the fecal microbiota of healthy dogs. It was partially confirmed that the antibacterial effect of tylosin, given the reduction of some microbial populations and metabolites, e.g., volatile fatty acids. Interestingly, the association of tylosin with prebiotics revealed counteracting effects on some undesirable changes exerted by tylosin, e.g., the reduction of bacteria generally considered beneficial such as lactobacilli and Clostridium cluster XIVa as well as volatile fatty acids, i.e., microbial fermentative end-products that are recognized as essential for enterocytes homeostasis.