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Article

Dietary Supplementation with Chitosan Oligosaccharides Alleviates Oxidative Stress in Rats Challenged with Hydrogen Peroxide

by
Ruixia Lan
,
Qingqing Chang
,
Lilong An
and
Zhihui Zhao
*
Department of Animal Science, College of Agriculture, Guangdong Ocean University, Zhanjiang 524-088, China
*
Author to whom correspondence should be addressed.
Animals 2020, 10(1), 55; https://doi.org/10.3390/ani10010055
Submission received: 5 December 2019 / Revised: 21 December 2019 / Accepted: 23 December 2019 / Published: 26 December 2019
(This article belongs to the Section Animal Physiology)
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Abstract

:

Simple Summary

Oxidative stress adversely affects animal health and performance. Feed additives with antioxidant abilities supplementation can alleviate oxidative stress. The aim of this study was to evaluate the hypothesis that dietary supplementation with COS alleviates the damage caused by oxidative stress in Sprague Dawley rats challenged with hydrogen peroxide (H2O2). The results shown that COS exhibited better radical scavenging capacity of 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2−), H2O2, and ferric ion reducing antioxidant power (FRAP) than butylated hydroxy anisole (BHA), increasing activity of SOD, CAT, GSH-Px, GSH, and T-AOC, as well as decreasing MDA level in serum, liver, spleen, and kidney. Our results indicated that COS can protect Sprague Dawley rats from H2O2 challenge by reducing lipid peroxidation and restoring antioxidant capacity.

Abstract

Oxidative stress is induced by excessive oxidative radicals, which directly react with biomolecules, and damage lipids, proteins and DNA, leading to cell or organ injury. Supplementation of antioxidants to animals can be an effective way to modulate the antioxidant system. Chitosan oligosaccharides (COS) are the degraded products of chitosan or chitin, which has strong antioxidant, anti-inflammatory, and immune-enhancing competency. Therefore, the current study was conducted to evaluate the hypothesis that dietary supplementation with COS alleviates the damage caused by oxidative stress in Sprague Dawley rats challenged with hydrogen peroxide (H2O2). The rats were randomly divided into three groups: CON, control group, in which rats were fed a basal diet with normal drinking water; AS, H2O2 group, in which rats were fed the basal diet and 0.1% H2O2 in the drinking water; ASC, AS + COS group, in which rats were fed the basal diet with 200 mg/kg COS, and with 0.1% H2O2 in the drinking water. In vitro, COS exhibited better radical scavenging capacity of 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion (O2−), H2O2, and ferric ion reducing antioxidant power (FRAP) than butylated hydroxy anisole (BHA). In vivo, dietary supplementation with COS alleviated the H2O2-induced oxidative damage, evidenced by comparatively increasing activity of SOD, CAT, GSH-Px, GSH, and T-AOC, and comparatively decreasing level of MDA in serum, liver, spleen, and kidney. COS also comparatively alleviated the H2O2-induced inflammation. In conclusion, COS supplementation reduced lipid peroxidation and restored antioxidant capacity in Sprague Dawley rats, which were challenged with H2O2.

1. Introduction

Oxidative stress is induced by excessive oxidative radicals, which directly react with biomolecules, damaging lipids, proteins, and DNA, and leading to cell or organ injury [1,2,3,4]. Moreover, oxidative stress disturbs the normal intestinal barrier function, decreases the expression of tight junction proteins, and reduces nutrient absorption and metabolism, resulting in growth depression [5,6,7,8,9]. Oxidative stress is also associated with health disorders, such as inflammatory disease, diabetes, and cancer [10,11]. During the past decades, evaluation of free radical generation and antioxidant defense have been widely investigated in animals [12]. Numerous studies reported that dietary antioxidant supplementation, such as Vitamin C, Vitamin E, curcumin, and Forsythia suspense extract, given to animals is a useful way to alleviate oxidative stress [13,14,15,16]. Thus, dietary antioxidant supplementation may be a useful way to modulate the antioxidant system. Chitosan oligosaccharides (COS) are the degraded products of chitosan or chitin [17], which can be used as a natural antioxidants [18] due to its strong antioxidant competency [19,20]. Furthermore, COS exhibit other biological activities, such as anti-bacterial [21,22], anti-inflammatory [23,24,25], and immune-enhancing activities [26,27]. The aim of this study was to evaluate the hypothesis that dietary supplementation with COS alleviates the oxidative stress in Sprague Dawley rats challenged with hydrogen peroxide (H2O2).

2. Materials and Methods

2.1. Animal Ethics

The experimental protocol used in this study was approved by the Animal Care and Use Committee of Guangdong Ocean University, China (SYXK-2018-0147).

2.2. Chemicals and Reagents

Chitosan oligosaccharides were purchased from Jiangsu Xinrui Biotechnology Co., Ltd. (HPLC purity 95%, deacetylation degree over 95% and average molecular weight below 32 kDa; Xuzhou, Jiangsu, China). We purchased 1, 1-diphenyl-2-picrylhydrazyl (DPPH), phenazine methosulfate (PMS), nicotinamide adenine dinucleotide (NADH), nitroblue tetrazolium (NBT), 2, 4, 6-tri (2-pyridyl)-s-triazine (TPTZ), 2, 2′-azobis (2-amidinopropane) dihydrochloride (AAPH), and butylated hydroxy anisole (BHA) from Sigma-Aldrich (Shanghai, China).
The kits used for analyzing the activity/level of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione (GSH), total antioxidant capacity (T-AOC), the content of malondialdehyde (MDA), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), and BCA protein were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China). Other chemicals were from Shanghai chemical agents’ company, China.

2.3. DPPH, O2−, H2O2, and FRAP Radical Scavenging Capacity

The DPPH, superoxide anion (O2−), H2O2, and ferric ion reducing antioxidant power (FRAP) radical scavenging capacity of COS and BHA were measured by the method described by Hou et al. [28]. Briefly, DPPH was dissolved in ethanol to a 0.1 mM solution. The sample and DPPH at 0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 g/L were mixed vigorously. After incubation at room temperature in the darkness for 30 min, the absorbance was determined at 517 nm. The scavenging effect was calculated by the equation: DPPH scavenging ability (%) = (Acontrol − Asample)/Acontrol × 100%. For analyzing the O2− scavenging activity, PMS, NADH and NBT were dissolved in phosphate buffer (PBS) to a 60,468 and 150 μM solution, respectively. The reaction mixture included 1 mL of sample solution at 0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 g/L, and 1 mL of PMS, NADH, and NBT solutions, and was then incubated for 5 min at room temperature and the absorbance was determined at 560 nm. The scavenging effect was calculated by the equation: O2− scavenging ability (%) = (Acontrol − Asample)/Acontrol × 100%. For analyzing H2O2 scavenging activity, a 43 mM H2O2 solution was prepared in PBS. Then 3.4 mL of sample solutions at 0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 g/L was mixed with 0.6 mL of a 43 mM H2O2 solution, and the absorbance was determined at 230 nm. The scavenging effect was calculated by the equation: H2O2 scavenging ability (%) = (Acontrol − Asample)/Acontrol × 100%. For FRAP analyzing, the FRAP reagent consisted of acetate buffer (0.3 M, pH = 3.6), TPTZ (10 mM) in hydrochloric acid (40 mM), and ferric chloride (20 mM), at a ratio of 10:1:1. The FRAP reagent was prepared just before the reaction, then 1.5 mL of FRAP reagent was mixed with 150 μL distilled water and 50 μL sample solutions at 0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 g/L. It was then incubated for 30 min at room temperature in the darkness and the absorbance was determined at 593 nm. The reducing power is presented as micromoles Fe/gram fresh weight.

2.4. Animals, Experiment Design, and Diets

The male Sprague Dawley rats (8–10 weeks, 178.39 ± 5.12 g) used in this study were obtained from the Beijing Administration Office of Laboratory Animals (Beijing, China). The rats were individually housed in polycarbonate cages with soft wood granulate floors, and kept at 24 °C, with a 12 h light–dark cycle. After a week of acclimatization, 30 rats were randomly divided into one of 3 groups with 10 rats in each group for this 10 day experiment: CON, control group, rats were fed basal diet and normal drinking water; AS, H2O2 group, rats were fed basal diet with 0.1% H2O2 in drinking water [29]; ASC, AS + COS group, rats were fed basal diet with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. The supplementation level of COS was based on our previous preliminary research. All rats had free access to water and diet. The composition of the basal diet is listed in Table 1, and was made according to the nutritional requirement recommendations by the American Institute of Nutrition-93 diet [30].

2.5. Plasma Collection and Tissue Preparation

On the last day of the experiment, after 12 h fasting, all rats were euthanized with diethyl ether. Blood was collected from the posterior vena orbitalis, then centrifuged at 3000× g × 10 min to collect the serum, and stored at −20 °C until analysis. The liver, kidney, and spleen were collected and a 10% homogenate was prepared in PBS and centrifuged at 3000× g × 10 min at 4 °C, the supernatant was used for further biochemical assays.

2.6. Antioxidant and Inflammatory Cytokines Assays

The content or the activity of MDA, SOD, CAT, GSH-Px, GSH, T-AOC, IL-1β, IL-6, IL-10, and TNF-α in serum, liver, kidney, and spleen were measured according to the manufacturer’s instruction.

2.7. Statistical Analysis

Data were analyzed by ANOVA using the GLM procedures of SAS (V9.1, SAS Inst., Inc., Cary, NC, USA). Duncan’s multiple range tests were done to check the differences among treatments, and p < 0.05 was considered significant.

3. Results

3.1. DPPH, O2−, H2O2, and FRAP Scavenging Capacity

The DPPH, O2−, H2O2, and FRAP scavenging capacity of COS and BHA is shown in Figure 1. The concentration ranges from 0.01 to 0.06 g/L, the scavenging activity of COS ranges from 50.19% to 61.77%, and the scavenging activity of BHA ranges from 24.52% to 36.10%. For O2− scavenging capacity, the scavenging activity of COS ranges from 32.94% to 40.31%, and the scavenging activity of BHA ranges from 23.45% to 30.02%. For the H2O2 scavenging capacity, the scavenging activity of COS ranges from 61.65% to 86.21%, and the scavenging activity of BHA ranges from 38.07% to 49.10%. For ferric reducing antioxidant power (FRAP) scavenging capacity, the scavenging activity of COS ranges from 44.24% to 42.62%, and the scavenging activity of BHA ranges from 23.73% to 24.93%.

3.2. Effects of COS on Antioxidant Status in Serum

Administration of H2O2 in drinking water increased (p < 0.05) the content of MDA in serum (Figure 2A), and decreased (p < 0.05) the activity of SOD (Figure 2B) and CAT (Figure 2C) in the AS group compared with the CON group. No significant differences were observed on the activity of MDA, SOD, CAT, GSH-Px, GSH, or T-AOC.

3.3. Effects of COS on Antioxidant Status in the Liver

The activity of SOD and GSH in the AS group was significantly (p < 0.05) lower than that in the CON group (Figure 3B,E) in the liver. The activity of CAT (Figure 3C), GSH-Px (Figure 3D), and T-AOC (Figure 3F), and the content of MDA (Figure 3A) were not significantly affected by exposure to H2O2 or supplementation with COS.

3.4. Effects of COS on Antioxidant Status in the Spleen

The activity of CAT and GSH-Px in the AS group was significantly (p < 0.05) lower than that in the CON group (Figure 4C,D) in the spleen. However, the activity of SOD (Figure 4B), GSH (Figure 4E), and T-AOC (Figure 4F), and the content of MDA (Figure 4A), were not significantly affected by exposure to H2O2 or supplementation with COS.

3.5. Effects of COS on Antioxidant Status in the Kidney

The activity of SOD, GSH-Px, GSH, and T-AOC in the AS group was significantly (p < 0.05) lower than that in the CON group (Figure 5B–F, respectively), the activity of GSH and T-AOC in the AS group was also lower (p < 0.05) than that in the ASC group. The activity of CAT (Figure 5C) and the content of MDA (Figure 4A) in the kidney were not significantly affected by exposure to H2O2 or supplementation with COS.

3.6. Effects of COS on Inflammatory Cytokines in the Serum, Liver, Spleen, and Kidney

The content of IL-1β, IL-6, TNF-α, and IL-10 in the serum, liver, spleen, and kidney are presented in Figure 6, Figure 7, Figure 8 and Figure 9, respectively. The content of IL-1β (Figure 6A, Figure 7A, Figure 8A and Figure 9A), IL-6 (Figure 6B, Figure 7B, Figure 8B and Figure 9B), IL-10 (Figure 6C, Figure 7C and Figure 9C), and TNF-α (Figure 6D, Figure 7D, Figure 8D and Figure 9D) in the serum, liver, spleen, and kidney did not differ among treatments, except the content of IL-10 (Figure 8C) in the spleen. The amount of IL-10 was significantly lower (p < 0.05) in the AS group compared with the CON group.

4. Discussion

Increasing attention has been paid to the field of antioxidants in recent years. Food scientists have paid attention to antioxidants because of their ability to prevent fat from oxidative rancidity. Doctors are interested because of their ability to protect from oxidative injury. COS possess stronger antioxidant competency as evidenced by their reaction with unstable free radicals to form stable radicals [31]. In the present study, the effects of COS on antioxidant property were analyzed both in vitro and in vivo.
DPPH is a stable nitrogen radical, and is widely used to evaluate radical quenching capacities. The DPPH scavenging ability of COS was higher than BHA. H2O2, and O2− act as signaling intermediates, and the over-production of H2O2 and O2− is an indicator of oxidative stress [32]. DPPH reflects limited oxidation situations because it only exists in vitro; therefore, we further evaluated the H2O2 and O2− scavenging capacity of COS. H2O2 can be synthesized and destroyed in response to external stimuli [33], and O2− acts as a potential precursor to generate reactive radical species. Thus, evaluating the scavenging capacity of H2O2 and O2− [34] is important for clarifying the antioxidant capacity. The results showed that the scavenging capacity of COS against H2O2 and O2− was higher than BHA. There is an association between antioxidant capacity of antioxidants and their reducing power. We investigated the reducing power of COS using the FRAP assay. The results showed the scavenging capacity of COS against FRAP was higher than BHA. The antioxidant capacity of COS is related to its characteristic structure, including its deacetylation ratio, molecular weight, and the source of the material. The molecular weight and deacetylation ratio of COS also exerts some synergistic effects on the biological capacities, where, generally, the low molecular weight gives the stronger scavenging activity of DPPH, H2O2 and O2− [35].
Many reports indicated that oxidative stress was related to animal health [36]. H2O2 is generally used as an oxidative stress stimulus, and studies have indicated that oral administration or intraperitoneal administration of H2O2 induces oxidative stress [37,38]. The detrimental effects of H2O2 depends on its conversation to hydroxy ions and other subsequent redox products. In this study, H2O2 challenge increased the content of MDA in the serum, liver, and kidney. Increasing content of MDA is an indicator of lipid peroxidation. COS supplementation resulted in a comparative decrease in MDA content in the serum, liver, and kidney, indicating that COS had protective effects due to its antioxidant capacity [39].
Both SOD and CAT can scavenge superoxide ions and hydroxyl ions. The present study showed decreasing activity of SOD in the serum, liver and kidney, and decreasing activity of CAT in the serum and spleen of H2O2-exposed rats. H2O2 induced a dramatic decrease in the activity of SOD and CAT, which may be related to the formation of reactive oxygen species (ROS) [29]. Dietary COS increased the activity of SOD and CAT, which can save the depletion of the two enzymes. GSH-Px is a glutathione-related enzyme, and there was a significant decrease in the activity of GSH-Px in the spleen and kidney of H2O2-exposed rats in this study. H2O2 can efficiently be scavenged by GSH-Px; therefore, the decreasing activity of GSH-Px reflects perturbations in the normal oxidative balance by H2O2 exposure. GSH is a typical non-enzyme antioxidant, which defends against reactive free radicals and other oxidant species in cellular defense systems. In this study, there was a significant decrease in the activity of GSH in the liver and kidney of H2O2-exposed rats, indicating that the depletion of GSH led to enhanced formation of ROS [40]. Meanwhile, COS supplementation increased the activity of GSH in the kidney, which suggests that COS maintained the redox balance of ROS through both the enzymatic and non-enzymatic antioxidant defense system. The value of T-AOC reflects the total antioxidant capacity [41]. In this study, the T-AOC value of the kidney decreased by H2O2 exposure, but increased with COS supplementation, which suggests that COS supplementation can alleviate oxidative stress induced by H2O2 through the non-enzymatic antioxidant system in the kidney. Both in vitro and in vivo experiments showed that COS has strong radical scavenging activity and antioxidant capacity. The radical scavenging activity of COS is associated with their proton donation ability [42]. Dietary supplementation with COS prevented H2O2-induced lipid peroxidation and reserved depletion of SOD, CAT, GSH-Px, and GSH activity, which was consistent with former reports indicating antioxidant and protective properties of COS [43,44,45].
Oxidative stress and inflammation are highly related [46]. Oxidative stress and excessive production of ROS is associated with inflammation, leading to the synthesis and release of pro-inflammatory cytokines. No significant differences were detected in the concentration of TNF-α, IL-1β, IL-6, and IL-10 in the serum, liver, spleen and kidney, except a significant decreasing concentration of IL-10 in the AS group compared to the CON group in the spleen. With COS supplementation, the level of TNF-α, IL-1β, and IL-6 in the ASC group was comparatively lower than that in the AS group in the serum, liver, spleen, and kidney, while the level of IL-10 in the ASC group was comparatively higher than that in the AS group, which may be due to the anti-inflammatory activity of COS [24].

5. Conclusions

In conclusion, COS had higher antioxidant activities than BHA when checked by DPPH, O2−, H2O2, and FRAP scavenging capacity in vitro. COS significantly increased the content of GSH and T-AOC in the kidney, and comparatively decreased the content of MDA in the serum, liver and kidney, which suggested COS had protective effects against H2O2-induced oxidative damage and can be used as a potential antioxidant in feed.

Author Contributions

R.L., L.A. and Z.Z. participated in the experiment design and manuscript writing. R.L. and Q.C. involved in feeding trail, sample and data collection, and analysis. All authors have read and agreed to the published version of the manuscript.

Funding

The work was sponsored by the scientific research start-up funds of Guangdong Ocean University (101402/R18005) and Key Platform Project of Innovative strong school Engineering by Department of Education of Guangdong Province (2018302).

Acknowledgments

The authors want to thank Guangzhen Guo, who assisted with rat management.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Brieger, K.; Schiavone, S.; Miller, J.; Francis, J.; Krause, K.H. Reactive oxygen species: From health to disease. Swiss Med. Wkly. 2012, 142, w13659. [Google Scholar] [CrossRef] [PubMed]
  2. Circu, M.L.; Aw, T.Y. Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic. Biol. Med. 2010, 48, 749–762. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. Kim, Y.J.; Kim, E.H.; Hahm, K.B. Oxidative stress in inflammation–based gastrointestinal tract diseases: Challenges and opportunities. J. Gastroen. Hepatol. 2012, 27, 1004–1010. [Google Scholar] [CrossRef] [PubMed]
  4. Muanprasat, C.; Chatsudthipong, V. Chitosan oligosaccharide: Biological activities and potential therapeutic applications. Pharmacol. Therapeut. 2017, 170, 80–97. [Google Scholar] [CrossRef] [PubMed]
  5. Yuan, S.B.; Chen, D.W.; Zhang, K.Y.; Yu, B. Effects of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidative enzymes of weanling pigs. Asian-Austral. J. Anim. Sci. 2007, 20, 1600–1605. [Google Scholar] [CrossRef]
  6. Lackeyram, D.; Mine, Y.; Widowski, T.; Archbold, T.; Fan, M. The in vivo infusion of hydrogen peroxide induces oxidative stress and differentially affects the activities of small intestinal carbohydrate digestive enzymes in the neonatal pig. J. Anim. Sci. 2012, 90, 418–420. [Google Scholar] [CrossRef]
  7. Duan, J.; Yin, J.; Ren, W.; Wu, M.; Chen, S.; Cui, Z.; Wu, X.; Huang, R.; Li, T.; Yin, Y. Pyrrolidine dithiocarbamate restores gastric damages and suppressive autophagy induced by hydrogen peroxide. Free Radic. Res. 2015, 49, 210–218. [Google Scholar] [CrossRef]
  8. Fukui, A.; Naito, Y.; Handa, O.; Kugai, M.; Tsuji, T.; Yoriki, H.; Qin, Y.; Adachi, S.; Higashimura, Y.; Mizushima, K. Acetyl salicylic acid induces damage to intestinal epithelial cells by oxidation-related modifications of ZO-1. Am. J. Physiol. Liver Physiol. 2012, 303, G927–G936. [Google Scholar] [CrossRef]
  9. Zhu, L.; Xu, J.; Zhu, S.; Cai, X.; Yang, S.; Chen, X.; Guo, Q. Gene expression profiling analysis reveals weaning-induced cell cycle arrest and apoptosis in the small intestine of pigs. J. Anim. Sci. 2014, 92, 996–1006. [Google Scholar] [CrossRef]
  10. Reuter, S.; Gupta, S.C.; Chaturvedi, M.M.; Aggarwal, B.B. Oxidative stress, inflammation, and cancer: How are they linked? Free Radic. Biol. Med. 2010, 49, 1603–1616. [Google Scholar] [CrossRef] [Green Version]
  11. Jomova, K.; Valko, M. Advances in metal-induced oxidative stress and human disease. Toxicology 2011, 283, 65–87. [Google Scholar] [CrossRef] [PubMed]
  12. Zini, A.; De Lamirande, E.; Gagnon, C. Reactive oxygen species in semen of infertile patients: Levels of superoxide dismutase–and catalase–like activities in seminal plasma and spermatozoa. Int. J. Androl. 1993, 16, 183–188. [Google Scholar] [CrossRef] [PubMed]
  13. Kaminogawa, S. Effects of food components on intestinal flora, intestinal immune system and their mutualism. Biosci. Microflora 2010, 29, 69–82. [Google Scholar] [CrossRef] [Green Version]
  14. Lu, T.; Piao, X.; Zhang, Q.; Wang, D.; Piao, X.; Kim, S. Protective effects of Forsythia suspense extract against oxidative stress induced by diquat in rats. Food Chem. Toxicol. 2010, 48, 764–770. [Google Scholar] [CrossRef] [PubMed]
  15. Sankar, P.; Telang, A.G.; Manimaran, A. Protective effect of curcumin on cypermethrin-induced oxidative stress in Wistar rats. Exp. Toxicol. Pathol. 2012, 64, 487–493. [Google Scholar] [CrossRef] [PubMed]
  16. Ryan, M.J.; Dudash, H.J.; Docherty, M.; Geronilla, K.B.; Baker, B.A.; Haff, G.G.; Cutlip, R.G.; Alway, S.E. Vitamin E and C supplementation reduces oxidative stress, improves antioxidant enzymes and positive muscle work in chronically loaded muscles of aged rats. Exp. Gerontol. 2010, 45, 882–895. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  17. Naveed, M.; Phil, L.; Sohail, M.; Hasnat, M.; Baig, M.M.F.A.; Ihsan, A.U.; Shumzaid, M.; Kakar, M.U.; Husain, T.; Akabar, M. Chitosan oligosaccharide (COS): An overview. Int. J. Biol. Macromol. 2019, 129, 827–843. [Google Scholar] [CrossRef]
  18. Xie, W.; Xu, P.; Liu, Q. Antioxidant activity of water-soluble chitosan derivatives. Bioorg. Med. Chem. Lett. 2001, 11, 1699–1701. [Google Scholar] [CrossRef]
  19. Yang, F.; Luan, B.; Sun, Z.; Yang, C.; Yu, Z.; Li, X. Application of chitooligosaccharides as antioxidants in beer to improve the flavour stability by protecting against beer staling during storage. Biotechnol. Lett. 2017, 39, 305–310. [Google Scholar] [CrossRef]
  20. Laokuldilok, T.; Potivas, T.; Kanha, N.; Surawang, S.; Seesuriyachan, P.; Wangtueai, S.; Phimolsiripol, Y.; Regenstein, J.M. Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three different enzyme treatments. Food Biosci. 2017, 18, 28–33. [Google Scholar] [CrossRef]
  21. Liu, X.; Xia, W.; Jiang, Q.; Yu, P.; Yue, L. Chitosan oligosaccharide-N-chlorokojic acid mannich base polymer as a potential antibacterial material. Carbohyd. Polym. 2018, 182, 225–234. [Google Scholar] [CrossRef] [PubMed]
  22. Sun, T.; Qin, Y.; Xu, H.; Xie, J.; Hu, D.; Xue, B.; Hua, X. Antibacterial activities and preservative effect of chitosan oligosaccharide Maillard reaction products on Penaeus vannamei. Int. J. Biol. Macromol. 2017, 105, 764–768. [Google Scholar] [CrossRef] [PubMed]
  23. Wei, P.; Ma, P.; Xu, Q.S.; Bai, Q.H.; Gu, J.G.; Xi, H.; Du, Y.G.; Yu, C. Chitosan oligosaccharides suppress production of nitric oxide in lipopolysaccharide-induced N9 murine microglial cells In Vitro. Glycoconjugate. J. 2012, 29, 285–295. [Google Scholar] [CrossRef] [PubMed]
  24. Azuma, K.; Osaki, T.; Minami, S.; Okamoto, Y. Anticancer and anti-inflammatory properties of chitin and chitosan oligosaccharides. J. Funct. Biomater. 2015, 6, 33–49. [Google Scholar] [CrossRef] [PubMed]
  25. Yang, E.J.; Kim, J.G.; Kim, J.Y.; Kim, S.C.; Lee, N.H.; Hyun, C.G. Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells. Cent. Eur. J. Biol. 2010, 5, 95–102. [Google Scholar] [CrossRef]
  26. Chung, M.J.; Park, J.K.; Park, Y.I. Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE–antigen complex-stimulated RBL-2H3 cells and asthma model mice. Int. Immunopharmacol. 2012, 12, 453–459. [Google Scholar] [CrossRef] [PubMed]
  27. Zhang, P.; Liu, W.; Peng, Y.; Han, B.; Yang, Y. Toll like receptor 4 (TLR4) mediates the stimulating activities of chitosan oligosaccharide on macrophages. Int. Immunopharmacol. 2014, 23, 254–261. [Google Scholar] [CrossRef]
  28. Hou, X.; Zhang, J.; Ahmad, H.; Zhang, H.; Xu, Z.; Wang, T. Evaluation of antioxidant activities of ampelopsin and its protective effect in lipopolysaccharide-induced oxidative stress piglets. PLoS ONE 2014, 9, e108314. [Google Scholar] [CrossRef] [Green Version]
  29. Ganie, S.A.; Haq, E.; Hamid, A.; Masood, A.; Zargar, M.A. Long dose exposure of hydrogen peroxide (H2O2) in albino rats and effect of Podophyllum hexandrum on oxidative stress. Eur. Rev. Med. Pharmacol. Sci. 2011, 15, 906–915. [Google Scholar]
  30. Reeves, P.G. Components of the AIN-93 diets as improvements in the AIN-76A diet. J. Nutr. 1997, 127, 838S–841S. [Google Scholar] [CrossRef]
  31. Liaqat, F.; Eltem, R. Chitooligosaccharides and their biological activities: A comprehensive review. Carbohyd. Polym. 2018, 184, 243–259. [Google Scholar] [CrossRef] [PubMed]
  32. Blokhina, O.; Virolainen, E.; Fagerstedt, K.V. Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann. Bot. 2003, 91, 179–194. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Rhee, S.G.; Kang, S.W.; Jeong, W.J.; Chang, T.S.; Yang, K.S.; Woo, H.A. Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins. Curr. Opin. Cell Biol. 2005, 17, 183–189. [Google Scholar] [CrossRef] [PubMed]
  34. Persinger, R.L.; Poynter, M.E.; Ckless, K.; Janssen-Heininger, Y.M.W. Molecular mechanisms of nitrogen dioxide induced epithelial injury in the lung. Mol. Cell Biochem. 2002, 234, 71–80. [Google Scholar] [CrossRef] [PubMed]
  35. Anraku, M.; Gebicki, J.M.; Iohara, D.; Tomida, H.; Uekama, K.; Maruyama, T.; Hirayama, F.; Otagiri, M. Antioxidant activities of chitosans and its derivatives in In Vitro and In Vivo studies. Carbohyd. Polym. 2018, 199, 141–149. [Google Scholar] [CrossRef]
  36. He, L.; He, T.; Farrar, S.; Ji, L.; Liu, T.; Ma, X. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cell. Physiol. Biochem. 2017, 44, 532–553. [Google Scholar] [CrossRef]
  37. Ganie, S.A.; Ali, D.T.; Zargar, S.; Bhat, A.H.; Dar, K.B.; Masood, A.; Zargar, M.A. Crataegus songarica methanolic extract accelerates enzymatic status in kidney and heart tissue damage in albino rats and its In Vitro cytotoxic activity. Pharm. Biol. 2016, 54, 1246–1254. [Google Scholar] [CrossRef] [Green Version]
  38. Duan, J.; Yin, J.; Ren, W.; Liu, T.; Cui, Z.; Huang, X.; Wu, L.; Kim, S.W.; Liu, G.; Wu, X. Dietary supplementation with l-glutamate and l-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide. Amino Acids 2016, 48, 53–64. [Google Scholar] [CrossRef]
  39. Wan, J.; Jiang, F.; Xu, Q.; Chen, D.; Yu, B.; Huang, Z.; Mao, X.; Yu, J.; He, J. New insights into the role of chitosan oligosaccharide in enhancing growth performance, antioxidant capacity, immunity and intestinal development of weaned pigs. RSC Adv. 2017, 7, 9669–9679. [Google Scholar] [CrossRef] [Green Version]
  40. Yousef, M.; Saad, A.; El-Shennawy, L. Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food Chem. Toxicol. 2009, 47, 1176–1183. [Google Scholar] [CrossRef]
  41. Ren, W.; Yin, Y.; Liu, G.; Yu, X.; Li, Y.; Yang, G.; Li, T.; Wu, G. Effect of dietary arginine supplementation on reproductive performance of mice with porcine circovirus type 2 infection. Amino Acids 2012, 42, 2089–2094. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  42. Shon, Y.H.; Park, I.K.; Moon, I.S.; Chang, H.W.; Park, I.K.; Nam, K.S. Effect of chitosan oligosaccharide on 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in mice. Biol. Pharm. Bull. 2002, 25, 1161–1164. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Qiao, Y.; Bai, X.F.; Du, Y.G. Chitosan oligosaccharides protect mice from LPS challenge by attenuation of inflammation and oxidative stress. Int. Immunopharmacol. 2011, 11, 121–127. [Google Scholar] [CrossRef] [PubMed]
  44. Liu, H.T.; He, J.L.; Li, W.M.; Yang, Z.; Wang, Y.X.; Bai, X.F.; Yu, C.; Du, Y.G. Chitosan oligosaccharides protect human umbilical vein endothelial cells from hydrogen peroxide-induced apoptosis. Carbohyd. Polym. 2010, 80, 1062–1071. [Google Scholar] [CrossRef]
  45. Xu, Q.; Ma, P.; Yu, W.; Tan, C.; Liu, H.; Xiong, C.; Qiao, Y.; Du, Y. Chitooligosaccharides protect human embryonic hepatocytes against oxidative stress induced by hydrogen peroxide. Mar. Biotechnol. 2010, 12, 292–298. [Google Scholar] [CrossRef]
  46. Tan, D.; Wang, Y.; Bai, B.; Yang, X.; Han, J. Betanin attenuates oxidative stress and inflammatory reaction in kidney of paraquat-treated rat. Food Chem. Toxicol. 2015, 78, 141–146. [Google Scholar] [CrossRef]
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Figure 1. DPPH, O2−, H2O2, and FRAP radical scavenging capacity of COS and BHA at 0.01–0.06 g/L. Values expressed as mean ± standard error of three parallel experiments. (A) DPPH scavenging capacity; (B) O2− scavenging capacity; (C) H2O2 scavenging capacity; (D) FRAP scavenging capacity.
Figure 1. DPPH, O2−, H2O2, and FRAP radical scavenging capacity of COS and BHA at 0.01–0.06 g/L. Values expressed as mean ± standard error of three parallel experiments. (A) DPPH scavenging capacity; (B) O2− scavenging capacity; (C) H2O2 scavenging capacity; (D) FRAP scavenging capacity.
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Figure 2. Effects of COS on antioxidant status in serum. Values expressed as mean ± standard error, n = 10. a,b Different superscript letters means significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; GSH, glutathione; T-AOC, total antioxidant capacity. (A) the content of MDA; (B) the activity of SOD; (C) the activity of CAT; (D) the activity of GSH-Px; (E) the content of GSH; (F) the content of T-AOC.
Figure 2. Effects of COS on antioxidant status in serum. Values expressed as mean ± standard error, n = 10. a,b Different superscript letters means significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; GSH, glutathione; T-AOC, total antioxidant capacity. (A) the content of MDA; (B) the activity of SOD; (C) the activity of CAT; (D) the activity of GSH-Px; (E) the content of GSH; (F) the content of T-AOC.
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Figure 3. Effects of COS on antioxidant status in the liver. Values expressed as mean ± standard error, n = 10. a,b Different superscript letters mean significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; GSH, glutathione; T-AOC, total antioxidant capacity. (A) the content of MDA; (B) the activity of SOD; (C) the activity of CAT; (D) the activity of GSH-Px; (E) the content of GSH; (F) the content of T-AOC.
Figure 3. Effects of COS on antioxidant status in the liver. Values expressed as mean ± standard error, n = 10. a,b Different superscript letters mean significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; GSH, glutathione; T-AOC, total antioxidant capacity. (A) the content of MDA; (B) the activity of SOD; (C) the activity of CAT; (D) the activity of GSH-Px; (E) the content of GSH; (F) the content of T-AOC.
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Figure 4. Effects of COS on antioxidant status in the spleen. Values expressed as mean ± standard error, n = 10. a,b Different superscript letters mean significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; GSH, glutathione; T-AOC, total antioxidant capacity. (A) the content of MDA; (B) the activity of SOD; (C) the activity of CAT; (D) the activity of GSH-Px; (E) the content of GSH; (F) the content of T-AOC.
Figure 4. Effects of COS on antioxidant status in the spleen. Values expressed as mean ± standard error, n = 10. a,b Different superscript letters mean significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; GSH, glutathione; T-AOC, total antioxidant capacity. (A) the content of MDA; (B) the activity of SOD; (C) the activity of CAT; (D) the activity of GSH-Px; (E) the content of GSH; (F) the content of T-AOC.
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Figure 5. Effects of COS on antioxidant status in kidney. Values expressed as mean ± standard error, n = 10. a,b Different superscript letters mean significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; GSH, glutathione; T-AOC, total antioxidant capacity. (A) the content of MDA; (B) the activity of SOD; (C) the activity of CAT; (D) the activity of GSH-Px; (E) the content of GSH; (F) the content of T-AOC.
Figure 5. Effects of COS on antioxidant status in kidney. Values expressed as mean ± standard error, n = 10. a,b Different superscript letters mean significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; GSH, glutathione; T-AOC, total antioxidant capacity. (A) the content of MDA; (B) the activity of SOD; (C) the activity of CAT; (D) the activity of GSH-Px; (E) the content of GSH; (F) the content of T-AOC.
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Figure 6. Effects of COS on inflammatory cytokines in serum. Values presented as mean ± standard error, n = 10. CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. (A) the concentration of IL-1β; (B) the concentration of IL-6; (C) the concentration of IL-10; (D) the concentration of TNF-α.
Figure 6. Effects of COS on inflammatory cytokines in serum. Values presented as mean ± standard error, n = 10. CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. (A) the concentration of IL-1β; (B) the concentration of IL-6; (C) the concentration of IL-10; (D) the concentration of TNF-α.
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Figure 7. Effects of COS on inflammatory cytokines in the liver. Values presented as mean ± standard error, n = 10. CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. (A) the concentration of IL-1β; (B) the concentration of IL-6; (C) the concentration of IL-10; (D) the concentration of TNF-α.
Figure 7. Effects of COS on inflammatory cytokines in the liver. Values presented as mean ± standard error, n = 10. CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. (A) the concentration of IL-1β; (B) the concentration of IL-6; (C) the concentration of IL-10; (D) the concentration of TNF-α.
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Figure 8. Effects of COS on inflammatory cytokines in the spleen. Values presented as mean ± standard error, n = 10. a,b Different superscript letters mean significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. (A) the concentration of IL-1β; (B) the concentration of IL-6; (C) the concentration of IL-10; (D) the concentration of TNF-α.
Figure 8. Effects of COS on inflammatory cytokines in the spleen. Values presented as mean ± standard error, n = 10. a,b Different superscript letters mean significant differences (p < 0.05). CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. (A) the concentration of IL-1β; (B) the concentration of IL-6; (C) the concentration of IL-10; (D) the concentration of TNF-α.
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Figure 9. Effects of COS on inflammatory cytokines in the kidney. Values presented as mean ± standard error, n = 10. CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. (A) the concentration of IL-1β; (B) the concentration of IL-6; (C) the concentration of IL-10; (D) the concentration of TNF-α.
Figure 9. Effects of COS on inflammatory cytokines in the kidney. Values presented as mean ± standard error, n = 10. CON, basal diet with normal drinking water; AS, basal diet with 0.1% H2O2 in drinking water; ASC, basal diet supplemented with 200 mg/kg COS, and with 0.1% H2O2 in drinking water. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α. (A) the concentration of IL-1β; (B) the concentration of IL-6; (C) the concentration of IL-10; (D) the concentration of TNF-α.
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Table
Table 1. Dietary composition and nutrient content of the basal diet.
Table 1. Dietary composition and nutrient content of the basal diet.
Ingredients, %Basal Diet
Cornstarch46.40
Casein14.00
Dextrinized cornstarch15.50
Sucrose10.00
Soybean oil4.00
Cellulose acetate5.00
Mineral premix 13.50
Vitamin premix 21.00
L-Methionine0.18
L-Cystine0.18
Choline bitartrate0.23
Tert-butylhydroquinone0.01
Gross energy (MJ/kg)16.22
1 Mineral premix (mg/kg of premix): CaCO3, 3.70 × 105; KH2PO4, 1.96 × 105; K3C6H5O7·H2O, 7.08 × 104; NaCl, 7.4 × 104; K2SO4, 4.66 × 104; MgO, 2.4 × 104; FeC6H5O7H2O, 6.06 × 103; ZnCO3, 1.65 × 103; MnCO3, 630; CuCO3, 324; NaSiO3·9H2O, 1.45 × 103; CrK(SO4)12H2O, 275; LiCl, 17.4; H3BO3, 81.5; NaF, 63.5; NiCO3·2Ni(OH)2·4H2O, 30.6; NH4VO3, 6.6; sucrose was added to make a total of 1 kg.; 2 Vitamin premix (mg/kg of premix): Nicotinic, 3.0 × 103; calcium pantothenate, 1.6 × 103; pyridoxine hydrochloride, 700; thiamine hydrochloride, 600; riboflavin, 600; folic acid, 200; D-biotin, 20; cyanocobalamin, 2.5 × 103; a-tocopherol, 1.5 × 104; cholecalciferol, 250; phylloquinone, 75; sucrose was added to make a total of 1 kg.

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MDPI and ACS Style

Lan, R.; Chang, Q.; An, L.; Zhao, Z. Dietary Supplementation with Chitosan Oligosaccharides Alleviates Oxidative Stress in Rats Challenged with Hydrogen Peroxide. Animals 2020, 10, 55. https://doi.org/10.3390/ani10010055

AMA Style

Lan R, Chang Q, An L, Zhao Z. Dietary Supplementation with Chitosan Oligosaccharides Alleviates Oxidative Stress in Rats Challenged with Hydrogen Peroxide. Animals. 2020; 10(1):55. https://doi.org/10.3390/ani10010055

Chicago/Turabian Style

Lan, Ruixia, Qingqing Chang, Lilong An, and Zhihui Zhao. 2020. "Dietary Supplementation with Chitosan Oligosaccharides Alleviates Oxidative Stress in Rats Challenged with Hydrogen Peroxide" Animals 10, no. 1: 55. https://doi.org/10.3390/ani10010055

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