Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Screening Samples Sera
2.3. Culture Methods
2.4. IS900-qPCR, f57-qPCR, type specific-PCR
2.4.1. DNA Extraction from Feces, Tissues, and Liquid Culture Media
2.4.2. IS900-qPCR
2.4.3. Other PCRs employed
2.4.4. Pathological Examination
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sera | Faeces | Tissues | |
---|---|---|---|
Sheep | 496 | 339 | 4 |
Goats | 105 | 78 | 0 |
Total | 601 | 417 | 4 |
Number | Confirming IS900-qPCR | f57-qPCR | type specific-PCR | |
---|---|---|---|---|
N Samples | 28 | 12 | 8 | 6 ovine strains |
N Animals | 24 | 8 | 6 | 4 animals |
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De Grossi, L.; Santori, D.; Barone, A.; Abbruzzese, S.; Ricchi, M.; Marcario, G.A. Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method. Animals 2020, 10, 20. https://doi.org/10.3390/ani10010020
De Grossi L, Santori D, Barone A, Abbruzzese S, Ricchi M, Marcario GA. Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method. Animals. 2020; 10(1):20. https://doi.org/10.3390/ani10010020
Chicago/Turabian StyleDe Grossi, Luigi, Davide Santori, Antonino Barone, Silvia Abbruzzese, Matteo Ricchi, and Gaetana Anita Marcario. 2020. "Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method" Animals 10, no. 1: 20. https://doi.org/10.3390/ani10010020
APA StyleDe Grossi, L., Santori, D., Barone, A., Abbruzzese, S., Ricchi, M., & Marcario, G. A. (2020). Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method. Animals, 10(1), 20. https://doi.org/10.3390/ani10010020