RTG Signaling Sustains Mitochondrial Respiratory Capacity in HOG1-Dependent Osmoadaptation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Yeast Strains and Growth Conditions
2.2. Micro- and Batch-Culture Growth Assays
2.3. Spotting Assay
2.4. Quantitative PCR (qPCR)
2.5. Oxygen Consumption Measurements
2.6. Statistical Analysis
3. Results
3.1. Cell Sensitivity to Osmotic Stress Due to NaCl Treatment
3.2. Impairment of RTG Signaling Sensitizes Cells to High Osmostress in an HOG1-Dependent Manner
3.3. RTG Signaling Is Activated in Osmoadaptation
3.4. RTG Mutants Show Decreased Respiratory Capacity under Osmostress
4. Discussion
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yeast Strains | Specific Growth Rate (µmax h−1) | Doubling Time (h) | Growth Efficiency (%) |
---|---|---|---|
WT | 0.38 ± 0.02 | 2.0 ± 0.32 | - |
WT + NaCl | 0.17 ± 0.01 | 3.9 ± 0.71 | 87 ± 2 |
∆RTG2 | 0.39 ± 0.03 | 2.2 ± 0.07 | - |
∆RTG2 + NaCl | 0.12 ± 0.01 | 5.9 ± 0.12 | 80 ± 4 |
∆HOG1 | 0.32 ± 0.01 | 2.1 ± 0.4 | - |
∆HOG1 + NaCl | 0.08 ± 0.02 | 10.3 ± 0.2 | 44 ± 9 |
∆HOG1∆RTG2 | 0.29 ± 0.09 | 2.5 ± 0.78 | - |
∆HOG1∆RTG2 + NaCl | 0.12 ± 0.01 | 7.2 ± 0.2 | 56 ± 12 |
∆RTG3 | 0.37 ± 0.04 | 2.5 ± 0.14 | - |
∆RTG3 + NaCl | 0.12 ± 0.01 | 5.4 ± 0.71 | 80 ± 2 |
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Guaragnella, N.; Agrimi, G.; Scarcia, P.; Suriano, C.; Pisano, I.; Bobba, A.; Mazzoni, C.; Palmieri, L.; Giannattasio, S. RTG Signaling Sustains Mitochondrial Respiratory Capacity in HOG1-Dependent Osmoadaptation. Microorganisms 2021, 9, 1894. https://doi.org/10.3390/microorganisms9091894
Guaragnella N, Agrimi G, Scarcia P, Suriano C, Pisano I, Bobba A, Mazzoni C, Palmieri L, Giannattasio S. RTG Signaling Sustains Mitochondrial Respiratory Capacity in HOG1-Dependent Osmoadaptation. Microorganisms. 2021; 9(9):1894. https://doi.org/10.3390/microorganisms9091894
Chicago/Turabian StyleGuaragnella, Nicoletta, Gennaro Agrimi, Pasquale Scarcia, Clelia Suriano, Isabella Pisano, Antonella Bobba, Cristina Mazzoni, Luigi Palmieri, and Sergio Giannattasio. 2021. "RTG Signaling Sustains Mitochondrial Respiratory Capacity in HOG1-Dependent Osmoadaptation" Microorganisms 9, no. 9: 1894. https://doi.org/10.3390/microorganisms9091894