Colonization of Clostridium butyricum in Rats and Its Effect on Intestinal Microbial Composition
Abstract
:1. Introduction
2. Materials and Methods
2.1. C. butyricum Cultivation
2.2. Primers and PCR Amplification
2.3. Real-Time qPCR
2.4. Animals and Experimental Groups
2.5. Genomic DNA Extraction from Pure Culture Strains and Fecal Samples of Rat Intestinal
2.6. 16S rDNA V3-V4 Region Sequencing and Bioinformatics Analysis
2.7. Statistical Analysis
3. Results
3.1. Specificity of Primer
3.2. Quantitation of C. Butyricum Populations in Rat Intestinal
3.3. C. butyricum Changes the Composition of Rat Intestinal Flora
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Set | Primers Sequences (5′–3′) | Annealing Temp (°C) | Approximate Amplicon Size (bp) | Reference or Source | |
---|---|---|---|---|---|
1 | Cbtu-1F | GTGCCGCCGCTAACGCATTAAGTAT | 72 | 213 | [17] |
Cbtu-1R | ACCATGCACCACCTGTCTTCCTGCC | ||||
2 | Cbtu-2F | GCATCCAGCAGACTTAGCAG | 63 | 275 | This study |
Cbtu-2R | CTTCCGACTGTCTCATCTTC | ||||
3 | Cbtu-3F | CAATGGTTGTGAATGCTGAGG | 60 | 142 | This study |
Cbtu-3R | CCAACTATGCTCATTTCGCCC | ||||
4 | Cbtu-4F | CCTCAAATCCGCCTTCTGG | 58 | 102 | This study |
Cbtu-4R | GGATCTTGTTATCGTTCCG | ||||
5 | Cbtu-5F | GAGTCTGATTCGGTTGTGGC | 60 | 135 | This study |
Cbtu-5R | CCTCTCTTCCTTGAACTGGATG |
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Luo, X.; Kong, Q.; Wang, Y.; Duan, X.; Wang, P.; Li, C.; Huan, Y. Colonization of Clostridium butyricum in Rats and Its Effect on Intestinal Microbial Composition. Microorganisms 2021, 9, 1573. https://doi.org/10.3390/microorganisms9081573
Luo X, Kong Q, Wang Y, Duan X, Wang P, Li C, Huan Y. Colonization of Clostridium butyricum in Rats and Its Effect on Intestinal Microbial Composition. Microorganisms. 2021; 9(8):1573. https://doi.org/10.3390/microorganisms9081573
Chicago/Turabian StyleLuo, Xianshu, Qing Kong, Yuming Wang, Xuefeng Duan, Peng Wang, Chenman Li, and Yuchen Huan. 2021. "Colonization of Clostridium butyricum in Rats and Its Effect on Intestinal Microbial Composition" Microorganisms 9, no. 8: 1573. https://doi.org/10.3390/microorganisms9081573