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Recent Advances in the Physicochemical Properties and Biotechnological Application of Vitreoscilla Hemoglobin
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Article

Effects of Efficient Expression of Vitreoscilla Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in Ganoderma lucidum

by 1, 2,* and 1,*
1
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
2
Faculty of Science, Kunming University of Science and Technology, Kunming 650500, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Benjamin C. Stark
Microorganisms 2021, 9(8), 1551; https://doi.org/10.3390/microorganisms9081551
Received: 5 July 2021 / Revised: 19 July 2021 / Accepted: 19 July 2021 / Published: 21 July 2021
(This article belongs to the Special Issue Microbial Hemoglobins)
A Vitreoscilla hemoglobin (VHb) gene was efficiently expressed by the optimization of codons and intron addition in G. lucidum. Expression of the VHb gene was confirmed by genome PCR, quantitative real-time PCR and carbon monoxide (CO)-difference spectrum analysis in the transformant. The effects of the efficient expression of VHb gene on production, monosaccharide compostion, and antioxidant activity of G. lucidum exopolysaccharides were studied. The maximum production of exopolysaccharides in the VHb gene-bearing transformant was 1.63 g/L, which was 1.5-fold higher than expression in the wild-type strain. Efficient expression of the VHb gene did not change the monosaccharide composition or distribution of molecular weight, but it increased the mole percentage ratio of galactose and mannose in G. lucidum exopolysaccharide. Exopolysaccharides from the transformant had higher scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radical capacity and reducing power than those from the wild-type strain. These results may be helpful for increasing production and application of exopolysaccharides produced by G. lucidum fermentation. View Full-Text
Keywords: Ganoderma; Vitreoscilla hemoglobin; exopolysaccharide; production; monosaccharide composition; antioxidant activity Ganoderma; Vitreoscilla hemoglobin; exopolysaccharide; production; monosaccharide composition; antioxidant activity
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MDPI and ACS Style

Wang, Z.-X.; Li, N.; Xu, J.-W. Effects of Efficient Expression of Vitreoscilla Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in Ganoderma lucidum. Microorganisms 2021, 9, 1551. https://doi.org/10.3390/microorganisms9081551

AMA Style

Wang Z-X, Li N, Xu J-W. Effects of Efficient Expression of Vitreoscilla Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in Ganoderma lucidum. Microorganisms. 2021; 9(8):1551. https://doi.org/10.3390/microorganisms9081551

Chicago/Turabian Style

Wang, Zi-Xu, Na Li, and Jun-Wei Xu. 2021. "Effects of Efficient Expression of Vitreoscilla Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in Ganoderma lucidum" Microorganisms 9, no. 8: 1551. https://doi.org/10.3390/microorganisms9081551

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