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Epidemiology of Mucormycosis in India
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Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New

Department of Hygiene and Medical Microbiology, Medical University Innsbruck, 6020 Innsbruck, Austria
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Academic Editor: Anna Skiada
Microorganisms 2021, 9(7), 1518; https://doi.org/10.3390/microorganisms9071518
Received: 23 June 2021 / Revised: 12 July 2021 / Accepted: 14 July 2021 / Published: 16 July 2021
(This article belongs to the Special Issue Recent Advances and Future Perspectives on Mucormycoses)
Members of the order Mucorales may cause severe invasive fungal infections (mucormycosis) in immune-compromised and otherwise ill patients. Diagnosis of Mucorales infections and discrimination from other filamentous fungi are crucial for correct management. Here, we present an overview of current state-of-the-art mucormycosis diagnoses, with a focus on recent developments in the molecular field. Classical diagnostic methods comprise histology/microscopy as well as culture and are still the gold standard. Newer molecular methods are evolving quickly and display great potential in early diagnosis, although standardization is still missing. Among them, quantitative PCR assays with or without melt curve analysis are most widely used to detect fungal DNA in clinical samples. Depending on the respective assay, sequencing of the resulting PCR product can be necessary for genus or even species identification. Further, DNA-based methods include microarrays and PCR-ESI-MS. However, general laboratory standards are still in development, meaning that molecular methods are currently limited to add-on analytics to culture and microscopy. View Full-Text
Keywords: Mucorales; diagnosis; DNA; PCR; specificity; sensitivity; clinical evaluation; standardization; culture; microscopy Mucorales; diagnosis; DNA; PCR; specificity; sensitivity; clinical evaluation; standardization; culture; microscopy
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MDPI and ACS Style

Lackner, N.; Posch, W.; Lass-Flörl, C. Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New. Microorganisms 2021, 9, 1518. https://doi.org/10.3390/microorganisms9071518

AMA Style

Lackner N, Posch W, Lass-Flörl C. Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New. Microorganisms. 2021; 9(7):1518. https://doi.org/10.3390/microorganisms9071518

Chicago/Turabian Style

Lackner, Nina, Wilfried Posch, and Cornelia Lass-Flörl. 2021. "Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New" Microorganisms 9, no. 7: 1518. https://doi.org/10.3390/microorganisms9071518

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