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Article

An Internal Promoter Drives the Expression of a Truncated Form of CCC1 Capable of Protecting Yeast from Iron Toxicity

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ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
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ID16A Beamline, ESRF-The European Synchrotron, 38043 Grenoble, France
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Authors to whom correspondence should be addressed.
Academic Editors: Nicoletta Guaragnella, Anita Krisko Outeiro and Tiago Fleming
Microorganisms 2021, 9(6), 1337; https://doi.org/10.3390/microorganisms9061337
Received: 5 May 2021 / Revised: 11 June 2021 / Accepted: 15 June 2021 / Published: 20 June 2021
In yeast, iron storage and detoxification depend on the Ccc1 transporter that mediates iron accumulation in vacuoles. While deletion of the CCC1 gene renders cells unable to survive under iron overload conditions, the deletion of its previously identified regulators only partially affects survival, indicating that the mechanisms controlling iron storage and detoxification in yeast are still far from well understood. This work reveals that CCC1 is equipped with a complex transcriptional structure comprising several regulatory regions. One of these is located inside the coding sequence of the gene and drives the expression of a short transcript encoding an N-terminally truncated protein, designated as s-Ccc1. s-Ccc1, though less efficiently than Ccc1, is able to promote metal accumulation in the vacuole, protecting cells against iron toxicity. While the expression of the s-Ccc1 appears to be repressed in the normal genomic context, our current data clearly demonstrates that it is functional and has the capacity to play a role under iron overload conditions. View Full-Text
Keywords: yeast; iron; gene expression; alternative promoter; vacuole; transcription; toxicity; iron metabolism yeast; iron; gene expression; alternative promoter; vacuole; transcription; toxicity; iron metabolism
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MDPI and ACS Style

Amaral, C.; Vicente, C.T.; Caetano, S.M.; Gaspar-Cordeiro, A.; Yang, Y.; Cloetens, P.; Romão, C.V.; Rodrigues-Pousada, C.; Pimentel, C. An Internal Promoter Drives the Expression of a Truncated Form of CCC1 Capable of Protecting Yeast from Iron Toxicity. Microorganisms 2021, 9, 1337. https://doi.org/10.3390/microorganisms9061337

AMA Style

Amaral C, Vicente CT, Caetano SM, Gaspar-Cordeiro A, Yang Y, Cloetens P, Romão CV, Rodrigues-Pousada C, Pimentel C. An Internal Promoter Drives the Expression of a Truncated Form of CCC1 Capable of Protecting Yeast from Iron Toxicity. Microorganisms. 2021; 9(6):1337. https://doi.org/10.3390/microorganisms9061337

Chicago/Turabian Style

Amaral, Catarina, Cristina T. Vicente, Soraia M. Caetano, Ana Gaspar-Cordeiro, Yang Yang, Peter Cloetens, Célia V. Romão, Claudina Rodrigues-Pousada, and Catarina Pimentel. 2021. "An Internal Promoter Drives the Expression of a Truncated Form of CCC1 Capable of Protecting Yeast from Iron Toxicity" Microorganisms 9, no. 6: 1337. https://doi.org/10.3390/microorganisms9061337

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