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Article

Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces

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Institute for Infectious Diseases and Infection Control, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany
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Institute of Medical Microbiology, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Rémy A. Bonnin and Thierry Naas
Microorganisms 2021, 9(6), 1190; https://doi.org/10.3390/microorganisms9061190
Received: 4 May 2021 / Revised: 21 May 2021 / Accepted: 27 May 2021 / Published: 31 May 2021
(This article belongs to the Special Issue ß-lactamases)
Background: Here, we describe an integrative method to detect carbapenemase-producing Gram-negative bacteria (gn-Cp) on surfaces/fomites in the patient environment. We examined environmental samples from 28 patient rooms occupied with patients who were proven to be colonised with gn-Cp by rectal screening. Methods: We took samples after 24 h, 72 h and one week. For sampling, we divided the patient environment into four parts and took samples from near- and extended patient areas. To obtain a representative bacterial swab from a larger surface, such as the patient cabinet, we used Polywipes. Bacterial DNA was isolated. Carbapenemase was detected with specific qPCR primers. Results: With this culture- and molecular-based approach, we could control the effectiveness of cleaning and disinfection in everyday clinical practice. Therefore, we could track the spread of gn-Cp within the patient room. The number of positive detections fluctuated between 30.5% (mean value positive results after 72 h) and 35.2% (after 24 h and one week). Conclusion: The method used to detect multidrug-resistant bacteria in the environment of patients by using PolywipesTM is reliable and can therefore be used as an effective, new tool in hygiene and infection control. View Full-Text
Keywords: molecular detection; fomites; carbapenemase-producing Gram-negative bacteria; hygiene; patient’s room molecular detection; fomites; carbapenemase-producing Gram-negative bacteria; hygiene; patient’s room
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MDPI and ACS Style

Stein, C.; Lange, I.; Rödel, J.; Pletz, M.W.; Kipp, F. Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces. Microorganisms 2021, 9, 1190. https://doi.org/10.3390/microorganisms9061190

AMA Style

Stein C, Lange I, Rödel J, Pletz MW, Kipp F. Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces. Microorganisms. 2021; 9(6):1190. https://doi.org/10.3390/microorganisms9061190

Chicago/Turabian Style

Stein, Claudia, Isabel Lange, Jürgen Rödel, Mathias W. Pletz, and Frank Kipp. 2021. "Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces" Microorganisms 9, no. 6: 1190. https://doi.org/10.3390/microorganisms9061190

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