Novel Plant-Associated Acidobacteria Promotes Growth of Common Floating Aquatic Plants, Duckweeds

Yoneda, Yasuko; Yamamoto, Kyosuke; Makino, Ayaka; Tanaka, Yasuhiro; Meng, Xian-Ying; Hashimoto, Junko; Shin-ya, Kazuo; Satoh, Noriyuki; Fujie, Manabu; Toyama, Tadashi; Mori, Kazuhiro; Ike, Michihiko; Morikawa, Masaaki; Kamagata, Yoichi; Tamaki, Hideyuki

doi:10.3390/microorganisms9061133

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Novel Plant-Associated Acidobacteria Promotes Growth of Common Floating Aquatic Plants, Duckweeds

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Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Ibaraki, Japan

Bioproduction Research Institute, AIST, Sapporo 062-8517, Hokkaido, Japan

Department of Environmental Sciences, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu 400-8510, Yamanashi, Japan

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Japan Biological Informatics Consortium (JBiC), Koto-ku, Tokyo 135-0064, Japan

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Cellular and Molecular Biotechnology Research Institute, AIST, Koto-ku, Tokyo 135-0064, Japan

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Okinawa Institute of Science, Technology Graduate University (OIST), Kunigami-gun 904-0495, Okinawa, Japan

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Department of Civil and Environmental Engineering, Faculty of Engineering, University of Yamanashi, Kofu 400-8511, Yamanashi, Japan

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Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, Suita 565-0871, Osaka, Japan

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Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Hokkaido, Japan

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Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8577, Ibaraki, Japan

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Microbiology Research Center for Sustainability (MiCS), University of Tsukuba, Tsukuba 305-8572, Ibaraki, Japan

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Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan

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Contributed equally.

Academic Editors: Maria Maddalena Del Gallo and Annamaria Bevivino

Microorganisms 2021, 9(6), 1133; https://doi.org/10.3390/microorganisms9061133

Received: 7 May 2021 / Accepted: 20 May 2021 / Published: 24 May 2021

(This article belongs to the Special Issue Beneficial Microbiomes in Agriculture and Human Health: The Food Connection)

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Abstract

Duckweeds are small, fast growing, and starch- and protein-rich aquatic plants expected to be a next generation energy crop and an excellent biomaterial for phytoremediation. Despite such an importance, very little is known about duckweed–microbe interactions that would be a key biological factor for efficient industrial utilization of duckweeds. Here we first report the duckweed growth promoting ability of bacterial strains belonging to the phylum Acidobacteria, the members of which are known to inhabit soils and terrestrial plants, but their ecological roles and plant–microbe interactions remain largely unclear. Two novel Acidobacteria strains, F-183 and TBR-22, were successfully isolated from wild duckweeds and phylogenetically affiliated with subdivision 3 and 6 of the phylum, respectively, based on 16S rRNA gene sequence analysis. In the co-culture experiments with aseptic host plants, the F-183 and TBR-22 strains visibly enhanced growth (frond number) of six duckweed species (subfamily Lemnoideae) up to 1.8–5.1 times and 1.6–3.9 times, respectively, compared with uninoculated controls. Intriguingly, both strains also increased the chlorophyll content of the duckweed (Lemna aequinoctialis) up to 2.4–2.5 times. Under SEM observation, the F-183 and TBR-22 strains were epiphytic and attached to the surface of duckweed. Taken together, our findings suggest that indigenous plant associated Acidobacteria contribute to a healthy growth of their host aquatic plants. View Full-Text

Keywords: plant-growth promoting bacteria; Acidobacteria; duckweed; host–microbe interaction; co-culture

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MDPI and ACS Style

Yoneda, Y.; Yamamoto, K.; Makino, A.; Tanaka, Y.; Meng, X.-Y.; Hashimoto, J.; Shin-ya, K.; Satoh, N.; Fujie, M.; Toyama, T.; Mori, K.; Ike, M.; Morikawa, M.; Kamagata, Y.; Tamaki, H. Novel Plant-Associated Acidobacteria Promotes Growth of Common Floating Aquatic Plants, Duckweeds. Microorganisms 2021, 9, 1133. https://doi.org/10.3390/microorganisms9061133

AMA Style

Yoneda Y, Yamamoto K, Makino A, Tanaka Y, Meng X-Y, Hashimoto J, Shin-ya K, Satoh N, Fujie M, Toyama T, Mori K, Ike M, Morikawa M, Kamagata Y, Tamaki H. Novel Plant-Associated Acidobacteria Promotes Growth of Common Floating Aquatic Plants, Duckweeds. Microorganisms. 2021; 9(6):1133. https://doi.org/10.3390/microorganisms9061133

Chicago/Turabian Style

Yoneda, Yasuko, Kyosuke Yamamoto, Ayaka Makino, Yasuhiro Tanaka, Xian-Ying Meng, Junko Hashimoto, Kazuo Shin-ya, Noriyuki Satoh, Manabu Fujie, Tadashi Toyama, Kazuhiro Mori, Michihiko Ike, Masaaki Morikawa, Yoichi Kamagata, and Hideyuki Tamaki. 2021. "Novel Plant-Associated Acidobacteria Promotes Growth of Common Floating Aquatic Plants, Duckweeds" Microorganisms 9, no. 6: 1133. https://doi.org/10.3390/microorganisms9061133

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Novel Plant-Associated Acidobacteria Promotes Growth of Common Floating Aquatic Plants, Duckweeds

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