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Microorganisms
Volume 9
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10.3390/microorganisms9050995
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Article

Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics

by
Bruno Weiss
1,2,†,
Anna Carolina Oliveira Souza
1,2,†,
Milena Tavares Lima Constancio
1,2,
Danillo Oliveira Alvarenga
1,
Victor S. Pylro
3,
Lucia M. Carareto Alves
1,* and
Alessandro M. Varani
1,*
1
Departament of Technology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo 14884-900, Brazil
2
Graduate Program in Agricultural and Livestock Microbiology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, São Paulo 14884-900, Brazil
3
Microbial Ecology and Bioinformatics Laboratory, Department of Biology, Federal University of Lavras (UFLA), Lavras, Minas Gerais 37200-000, Brazil
*
Authors to whom correspondence should be addressed.
†
Both authors contributed equally to this work.
Microorganisms 2021, 9(5), 995; https://doi.org/10.3390/microorganisms9050995
Submission received: 22 March 2021 / Revised: 28 March 2021 / Accepted: 30 March 2021 / Published: 5 May 2021
(This article belongs to the Special Issue Biomass Deconstruction)
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Abstract

Second-generation biofuel production is in high demand, but lignocellulosic biomass’ complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber’s appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which ~46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., Chryseobacterium) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., Chitinophaga, Niastella, Niabella, and Siphonobacter). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortium.
Keywords: lignocellulose; biotechnology; metabolic modeling; biofuels; community dynamics lignocellulose; biotechnology; metabolic modeling; biofuels; community dynamics

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MDPI and ACS Style

Weiss, B.; Souza, A.C.O.; Constancio, M.T.L.; Alvarenga, D.O.; Pylro, V.S.; Alves, L.M.C.; Varani, A.M. Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics. Microorganisms 2021, 9, 995. https://doi.org/10.3390/microorganisms9050995

AMA Style

Weiss B, Souza ACO, Constancio MTL, Alvarenga DO, Pylro VS, Alves LMC, Varani AM. Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics. Microorganisms. 2021; 9(5):995. https://doi.org/10.3390/microorganisms9050995

Chicago/Turabian Style

Weiss, Bruno, Anna Carolina Oliveira Souza, Milena Tavares Lima Constancio, Danillo Oliveira Alvarenga, Victor S. Pylro, Lucia M. Carareto Alves, and Alessandro M. Varani. 2021. "Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics" Microorganisms 9, no. 5: 995. https://doi.org/10.3390/microorganisms9050995

APA Style

Weiss, B., Souza, A. C. O., Constancio, M. T. L., Alvarenga, D. O., Pylro, V. S., Alves, L. M. C., & Varani, A. M. (2021). Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics. Microorganisms, 9(5), 995. https://doi.org/10.3390/microorganisms9050995

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