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Comment published on 12 November 2021, see Microorganisms 2021, 9(11), 2345.
Article

Gene Sequencing and Phylogenetic Analysis: Powerful Tools for an Improved Diagnosis of Fish Mycobacteriosis Caused by Mycobacterium fortuitum Group Members

1
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Via Bologna 148, 10154 Torino, Italy
2
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, Via Vienna 2, 07100 Sassari, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Semih Esin
Microorganisms 2021, 9(4), 797; https://doi.org/10.3390/microorganisms9040797
Received: 28 February 2021 / Revised: 13 March 2021 / Accepted: 8 April 2021 / Published: 10 April 2021
The Mycobacterium fortuitum group (MFG) consists of about 15 species of fast-growing nontuberculous mycobacteria (NTM). These globally distributed microorganisms can cause diseases in humans and animals, especially fish. The increase in the number of species belonging to MFG and the diagnostic techniques panel do not allow to clarify their real clinical significance. In this study, biomolecular techniques were adopted for species determination of 130 isolates derived from fish initially identified through biochemical tests as NTM belonging to MFG. Specifically, gene sequencing and phylogenetic analysis were used based on a fragment of the gene encoding the 65 KDa heat shock protein (hsp65). The analyzes made it possible to confirm that all the isolates belong to MFG, allowing to identify the strains at species level. Phylogenetic analysis substantially confirmed what was obtained by gene sequencing, except for six strains; this is probably due to the sequences present in NCBI database. Although the methodology used cannot represent a univocal identification system, this study has allowed us to evaluate its effectiveness as regards the species of MFG. Future studies will be necessary to apply these methods with other gene fragments and to clarify the real pathogenic significance of the individual species of this group of microorganisms. View Full-Text
Keywords: Mycobacterium fortuitum; Mycobacterium peregrinum; Mycobacteria Other Than Tuberculosis (MOTT); Nontubercolus mycobacteria (NTM); fish mycobacteriosis; zoonoses; heat shock protein; Sanger sequencing; phylogenetic tree Mycobacterium fortuitum; Mycobacterium peregrinum; Mycobacteria Other Than Tuberculosis (MOTT); Nontubercolus mycobacteria (NTM); fish mycobacteriosis; zoonoses; heat shock protein; Sanger sequencing; phylogenetic tree
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MDPI and ACS Style

Mugetti, D.; Tomasoni, M.; Pastorino, P.; Esposito, G.; Menconi, V.; Dondo, A.; Prearo, M. Gene Sequencing and Phylogenetic Analysis: Powerful Tools for an Improved Diagnosis of Fish Mycobacteriosis Caused by Mycobacterium fortuitum Group Members. Microorganisms 2021, 9, 797. https://doi.org/10.3390/microorganisms9040797

AMA Style

Mugetti D, Tomasoni M, Pastorino P, Esposito G, Menconi V, Dondo A, Prearo M. Gene Sequencing and Phylogenetic Analysis: Powerful Tools for an Improved Diagnosis of Fish Mycobacteriosis Caused by Mycobacterium fortuitum Group Members. Microorganisms. 2021; 9(4):797. https://doi.org/10.3390/microorganisms9040797

Chicago/Turabian Style

Mugetti, Davide, Mattia Tomasoni, Paolo Pastorino, Giuseppe Esposito, Vasco Menconi, Alessandro Dondo, and Marino Prearo. 2021. "Gene Sequencing and Phylogenetic Analysis: Powerful Tools for an Improved Diagnosis of Fish Mycobacteriosis Caused by Mycobacterium fortuitum Group Members" Microorganisms 9, no. 4: 797. https://doi.org/10.3390/microorganisms9040797

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