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Article

CD4+ and CD8+ Circulating Memory T Cells Are Crucial in the Protection Induced by Vaccination with Salmonella Typhi Porins

1
Unidad de Investigación Médica en Inmunoquímica, Hospital de Especialidades del Centro Médico Nacional “Siglo XXI”, Instituto Mexicano del Seguro Social (IMSS), Cuauhtemoc, Ciudad de Mexico 06720, Mexico
2
Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Coyoacan, Ciudad de Mexico 04510, Mexico
3
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Miguel Hidalgo, Ciudad de Mexico 11340, Mexico
4
Consejo Nacional de Ciencia y Tecnología (CONACYT), Benito Juárez, Ciudad de Mexico 03940, Mexico
*
Authors to whom correspondence should be addressed.
Academic Editor: María-Jesús Grilló
Microorganisms 2021, 9(4), 770; https://doi.org/10.3390/microorganisms9040770
Received: 9 March 2021 / Revised: 23 March 2021 / Accepted: 29 March 2021 / Published: 7 April 2021
(This article belongs to the Special Issue Salmonella and Salmonellosis)
Salmonella enterica serovar Typhi (S. Typhi) porins, OmpC and OmpF, are potent inducers of the immune response against S. Typhi in mice and humans. Vaccination with porins induces the protection against 500 LD50 of S. Typhi, life-lasting bactericidal antibodies and effector T cell responses in mice; however, the nature of the memory T cell compartment and its contribution to protection remains unknown. In this work, we firstly observed that vaccination with porins induces in situ (skin) CD4+ and CD8+ T cell responses. Analysis of the porin-specific functional responses of skin CD4+ and CD8+ T cells showed IFN-gamma- and IL-17-producing cells in both T cell populations. The memory phenotype of porin-specific T cells indicated the presence of resident and effector memory phenotypes in the skin, and a central memory phenotype in the skin-draining lymph node. In addition, we demonstrated that vaccination with porins via skin reduces the bacterial burden following challenge. Finally, evaluating the role of the circulating T cell memory population in protection, we showed that circulating memory CD4+ and CD8+ T cells are crucial in porin-mediated protection against S. Typhi. Overall, this study highlights the importance of inducing circulating memory T cell responses in order to achieve the optimal protection provided by porins, showing a mechanism that could be sought in the rational development of vaccines. View Full-Text
Keywords: S. Typhi porins; vaccination; circulating memory; T cells S. Typhi porins; vaccination; circulating memory; T cells
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MDPI and ACS Style

Ontiveros-Padilla, L.; García-Lozano, A.; Tepale-Segura, A.; Rivera-Hernández, T.; Pastelin-Palacios, R.; Isibasi, A.; Arriaga-Pizano, L.A.; Bonifaz, L.C.; López-Macías, C. CD4+ and CD8+ Circulating Memory T Cells Are Crucial in the Protection Induced by Vaccination with Salmonella Typhi Porins. Microorganisms 2021, 9, 770. https://doi.org/10.3390/microorganisms9040770

AMA Style

Ontiveros-Padilla L, García-Lozano A, Tepale-Segura A, Rivera-Hernández T, Pastelin-Palacios R, Isibasi A, Arriaga-Pizano LA, Bonifaz LC, López-Macías C. CD4+ and CD8+ Circulating Memory T Cells Are Crucial in the Protection Induced by Vaccination with Salmonella Typhi Porins. Microorganisms. 2021; 9(4):770. https://doi.org/10.3390/microorganisms9040770

Chicago/Turabian Style

Ontiveros-Padilla, Luis, Alberto García-Lozano, Araceli Tepale-Segura, Tania Rivera-Hernández, Rodolfo Pastelin-Palacios, Armando Isibasi, Lourdes A. Arriaga-Pizano, Laura C. Bonifaz, and Constantino López-Macías. 2021. "CD4+ and CD8+ Circulating Memory T Cells Are Crucial in the Protection Induced by Vaccination with Salmonella Typhi Porins" Microorganisms 9, no. 4: 770. https://doi.org/10.3390/microorganisms9040770

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