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Article

A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis

1
Plymouth Marine Laboratory, Plymouth PL1 3DH, UK
2
Interface Analysis Centre, Wills Physics Laboratory, University of Bristol, Bristol BS8 1TL, UK
3
Department of Chemistry, Lancaster University, Lancaster LA1 4YB, UK
4
Materials Science Institute, Lancaster University, Lancaster LA1 4YB, UK
5
Department of Physics, Virginia Commonwealth University, Richmond, VA 23284, USA
6
Department of Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road EX4 4QD, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Etienne Dague
Microorganisms 2021, 9(4), 680; https://doi.org/10.3390/microorganisms9040680
Received: 22 February 2021 / Revised: 19 March 2021 / Accepted: 22 March 2021 / Published: 25 March 2021
Suitable immobilisation of microorganisms and single cells is key for high-resolution topographical imaging and study of mechanical properties with atomic force microscopy (AFM) under physiologically relevant conditions. Sample preparation techniques must be able to withstand the forces exerted by the Z range-limited cantilever tip, and not negatively affect the sample surface for data acquisition. Here, we describe an inherently flexible methodology, utilising the high-resolution three-dimensional based printing technique of multiphoton polymerisation to rapidly generate bespoke arrays for cellular AFM analysis. As an example, we present data collected from live Emiliania huxleyi cells, unicellular microalgae, imaged by contact mode High-Speed Atomic Force Microscopy (HS-AFM), including one cell that was imaged continuously for over 90 min. View Full-Text
Keywords: high-speed; atomic force microscopy; microalgae; microbe; immobilization; multiphoton polymerization; 3D printing high-speed; atomic force microscopy; microalgae; microbe; immobilization; multiphoton polymerization; 3D printing
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MDPI and ACS Style

Evans, C.T.; Baldock, S.J.; Hardy, J.G.; Payton, O.; Picco, L.; Allen, M.J. A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis. Microorganisms 2021, 9, 680. https://doi.org/10.3390/microorganisms9040680

AMA Style

Evans CT, Baldock SJ, Hardy JG, Payton O, Picco L, Allen MJ. A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis. Microorganisms. 2021; 9(4):680. https://doi.org/10.3390/microorganisms9040680

Chicago/Turabian Style

Evans, Christopher T., Sara J. Baldock, John G. Hardy, Oliver Payton, Loren Picco, and Michael J. Allen. 2021. "A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis" Microorganisms 9, no. 4: 680. https://doi.org/10.3390/microorganisms9040680

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