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Communication

A New SNP-Based Genotyping Method for C. psittaci: Application to Field Samples for Quick Identification

1
Laboratory for Animal Health, Bacterial Zoonosis Unit, ANSES Maisons-Alfort, Paris-Est University, 94706 Paris, France
2
Mycoplasma and Chlamydia Infections in Humans, University of Bordeaux, 33076 Bordeaux, France
3
Department of Cattle and Sheep Diseases, National Veterinary Research Institute, 24100 Pulawy, Poland
4
Department of Clinical Microbiology, Uppsala University Hospital, 75185 Uppsala, Sweden
5
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, 75123 Uppsala, Sweden
6
Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), 07743 Jena, Germany
*
Author to whom correspondence should be addressed.
Shared first authorship.
Academic Editor: Valentina Virginia Ebani
Microorganisms 2021, 9(3), 625; https://doi.org/10.3390/microorganisms9030625
Received: 1 February 2021 / Revised: 15 March 2021 / Accepted: 16 March 2021 / Published: 17 March 2021
(This article belongs to the Section Molecular Microbiology and Immunology)
Chlamydia (C.) psittaci is the causative agent of avian chlamydiosis and human psittacosis. In this study, we extracted single-nucleotide polymorphisms (SNPs) from the whole genome sequences of 55 C. psittaci strains and identified eight major lineages, most of which are host-related. A combined PCR/high-resolution melting (HRM) assay was developed to screen for eight phylogenetically informative SNPs related to the identified C. psittaci lineages. The PCR-HRM method was validated on 11 available reference strains and with a set of 118 field isolates. Overall, PCR-HRM clustering was consistent with previous genotyping data obtained by ompA and/or MLST analysis. The method was then applied to 28 C. psittaci-positive samples from animal or human cases. As expected, PCR-HRM typing results from human samples identified genotypes linked to ducks and pigeons, a common source of human exposure, but also to the poorly described Mat116-like genotype. The new genotyping method does not require time-consuming sequencing and allows a quick identification of the source of infection. View Full-Text
Keywords: Chlamydia psittaci; SNP; PCR-high-resolution melting (HRM); psittacosis; avian chlamydiosis; genotyping Chlamydia psittaci; SNP; PCR-high-resolution melting (HRM); psittacosis; avian chlamydiosis; genotyping
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MDPI and ACS Style

Vorimore, F.; Aaziz, R.; de Barbeyrac, B.; Peuchant, O.; Szymańska-Czerwińska, M.; Herrmann, B.; Schnee, C.; Laroucau, K. A New SNP-Based Genotyping Method for C. psittaci: Application to Field Samples for Quick Identification. Microorganisms 2021, 9, 625. https://doi.org/10.3390/microorganisms9030625

AMA Style

Vorimore F, Aaziz R, de Barbeyrac B, Peuchant O, Szymańska-Czerwińska M, Herrmann B, Schnee C, Laroucau K. A New SNP-Based Genotyping Method for C. psittaci: Application to Field Samples for Quick Identification. Microorganisms. 2021; 9(3):625. https://doi.org/10.3390/microorganisms9030625

Chicago/Turabian Style

Vorimore, Fabien, Rachid Aaziz, Bertille de Barbeyrac, Olivia Peuchant, Monika Szymańska-Czerwińska, Björn Herrmann, Christiane Schnee, and Karine Laroucau. 2021. "A New SNP-Based Genotyping Method for C. psittaci: Application to Field Samples for Quick Identification" Microorganisms 9, no. 3: 625. https://doi.org/10.3390/microorganisms9030625

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