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Article

Impact of the Histidine-Containing Phosphocarrier Protein HPr on Carbon Metabolism and Virulence in Staphylococcus aureus

1
Institute of Medical Microbiology and Hygiene, Saarland University, D-66421 Homburg, Germany
2
School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, NE 68588, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Rajan P. Adhikari
Microorganisms 2021, 9(3), 466; https://doi.org/10.3390/microorganisms9030466
Received: 7 February 2021 / Revised: 18 February 2021 / Accepted: 19 February 2021 / Published: 24 February 2021
(This article belongs to the Special Issue Staphylococcal Infections (Host and Pathogenic Factors))
Carbon catabolite repression (CCR) is a common mechanism pathogenic bacteria use to link central metabolism with virulence factor synthesis. In gram-positive bacteria, catabolite control protein A (CcpA) and the histidine-containing phosphocarrier protein HPr (encoded by ptsH) are the predominant mediators of CCR. In addition to modulating CcpA activity, HPr is essential for glucose import via the phosphotransferase system. While the regulatory functions of CcpA in Staphylococcus aureus are largely known, little is known about the function of HPr in CCR and infectivity. To address this knowledge gap, ptsH mutants were created in S. aureus that either lack the open reading frame or harbor a ptsH variant carrying a thymidine to guanosine mutation at position 136, and the effects of these mutations on growth and metabolism were assessed. Inactivation of ptsH altered bacterial physiology and decreased the ability of S. aureus to form a biofilm and cause infections in mice. These data demonstrate that HPr affects central metabolism and virulence in S. aureus independent of its influence on CcpA regulation. View Full-Text
Keywords: Staphylococcus aureus; physiology; metabolism; carbon catabolite repression; CcpA; HPr Staphylococcus aureus; physiology; metabolism; carbon catabolite repression; CcpA; HPr
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MDPI and ACS Style

Pätzold, L.; Brausch, A.-C.; Bielefeld, E.-L.; Zimmer, L.; Somerville, G.A.; Bischoff, M.; Gaupp, R. Impact of the Histidine-Containing Phosphocarrier Protein HPr on Carbon Metabolism and Virulence in Staphylococcus aureus. Microorganisms 2021, 9, 466. https://doi.org/10.3390/microorganisms9030466

AMA Style

Pätzold L, Brausch A-C, Bielefeld E-L, Zimmer L, Somerville GA, Bischoff M, Gaupp R. Impact of the Histidine-Containing Phosphocarrier Protein HPr on Carbon Metabolism and Virulence in Staphylococcus aureus. Microorganisms. 2021; 9(3):466. https://doi.org/10.3390/microorganisms9030466

Chicago/Turabian Style

Pätzold, Linda, Anne-Christine Brausch, Evelyn-Laura Bielefeld, Lisa Zimmer, Greg A. Somerville, Markus Bischoff, and Rosmarie Gaupp. 2021. "Impact of the Histidine-Containing Phosphocarrier Protein HPr on Carbon Metabolism and Virulence in Staphylococcus aureus" Microorganisms 9, no. 3: 466. https://doi.org/10.3390/microorganisms9030466

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