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Article

The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed Metschnikowia sp. Yeasts

1
Faculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech Republic
2
Institute of Scientific Instruments of the Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech Republic
3
Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, 4032 Debrecen, Hungary
4
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Academic Editor: Ilaria Mannazzu
Microorganisms 2021, 9(2), 277; https://doi.org/10.3390/microorganisms9020277
Received: 30 November 2020 / Revised: 25 January 2021 / Accepted: 26 January 2021 / Published: 29 January 2021
(This article belongs to the Special Issue Microbial Stress Response as a Tool for Biotechnology)
Raman spectroscopy is a universal method designed for the analysis of a wide range of physical, chemical and biological systems or various surfaces. This technique is suitable to monitor various components of cells, tissues or microorganisms. The advantages include very fast non-contact and non-destructive analysis and no or minimal need for sample treatment. The yeasts Metschnikowia can be considered as industrially usable producers of pulcherrimin or single-cell lipids, depending on cultivation conditions and external stress. In the present study, Raman spectroscopy was used as an effective tool to identify both pulcherrimin and lipids in single yeast cells. The analysis of pulcherrimin is very demanding; so far, there is no optimal procedure to analyze or identify this pigment. Based on results, the strong dependence of pulcherrimin production on the ferric ion concentration was found with the highest yield in media containing 0.1 g/L iron. Further, production of lipids in Metschnikowia cells was studied at different temperatures and C:N ratios, using Raman spectroscopy to follow fatty acids composition, under different regimes, by monitoring the iodine number. The results of Raman spectroscopy were comparable with the fatty acid analysis obtained by gas chromatography. This study therefore supported use of Raman spectroscopy for biotechnological applications as a simple tool in the identification and analysis both the pulcherrimin and microbial lipids. This method provides a quick and relatively accurate estimation of targeted metabolites with minimal sample modification and allows to monitor metabolic changes over time of cultivation. View Full-Text
Keywords: yeasts; Metschnikowia; lipids; pulcherrimin; Raman spectroscopy yeasts; Metschnikowia; lipids; pulcherrimin; Raman spectroscopy
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MDPI and ACS Style

Němcová, A.; Gonová, D.; Samek, O.; Sipiczki, M.; Breierová, E.; Márová, I. The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed Metschnikowia sp. Yeasts. Microorganisms 2021, 9, 277. https://doi.org/10.3390/microorganisms9020277

AMA Style

Němcová A, Gonová D, Samek O, Sipiczki M, Breierová E, Márová I. The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed Metschnikowia sp. Yeasts. Microorganisms. 2021; 9(2):277. https://doi.org/10.3390/microorganisms9020277

Chicago/Turabian Style

Němcová, Andrea, Dominika Gonová, Ota Samek, Matthias Sipiczki, Emilia Breierová, and Ivana Márová. 2021. "The Use of Raman Spectroscopy to Monitor Metabolic Changes in Stressed Metschnikowia sp. Yeasts" Microorganisms 9, no. 2: 277. https://doi.org/10.3390/microorganisms9020277

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