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Article

Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival of Pseudomonas brassicacerum in Nutrient-Poor Environments

1
Aix Marseille Univ, CEA, CNRS, BIAM, Lab Microbial Ecology of the Rhizosphere (LEMiRE), F-13108 Saint-Paul-Lez-Durance, France
2
Université de Strasbourg, CNRS, ARN UPR 9002, F-67000 Strasbourg, France
3
Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, 85764 Neuherberg, Germany
4
Technical University of Munich, Maximus-von-Imhof-Forum 2, 85354 Freising, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Jacob G. Malone
Microorganisms 2021, 9(2), 250; https://doi.org/10.3390/microorganisms9020250
Received: 1 January 2021 / Revised: 13 January 2021 / Accepted: 19 January 2021 / Published: 26 January 2021
(This article belongs to the Special Issue Plant-Associated Pseudomonads)
In the beneficial plant root-associated Pseudomonas brassicacearum strain NFM421, the GacS/GacA two-component system positively controls biofilm formation and the production of secondary metabolites through the synthesis of rsmX, rsmY and rsmZ. Here, we evidenced the genetic amplification of Rsm sRNAs by the discovery of a novel 110-nt long sRNA encoding gene, rsmX-2, generated by the duplication of rsmX-1 (formerly rsmX). Like the others rsm genes, its overexpression overrides the gacA mutation. We explored the expression and the stability of rsmX-1, rsmX-2, rsmY and rsmZ encoding genes under rich or nutrient-poor conditions, and showed that their amount is fine-tuned at the transcriptional and more interestingly at the post-transcriptional level. Unlike rsmY and rsmZ, we noticed that the expression of rsmX-1 and rsmX-2 genes was exclusively GacA-dependent. The highest expression level and longest half-life for each sRNA were correlated with the highest ppGpp and cyclic-di-GMP levels and were recorded under nutrient-poor conditions. Together, these data support the view that the Rsm system in P. brassicacearum is likely linked to the stringent response, and seems to be required for bacterial adaptation to nutritional stress. View Full-Text
Keywords: Rsm sRNAs; Pseudomonas brassicacearum; sRNAs stability; nutritional stress; GacA-dependent Rsm sRNAs; Pseudomonas brassicacearum; sRNAs stability; nutritional stress; GacA-dependent
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MDPI and ACS Style

Lalaouna, D.; Fochesato, S.; Harir, M.; Ortet, P.; Schmitt-Kopplin, P.; Heulin, T.; Achouak, W. Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival of Pseudomonas brassicacerum in Nutrient-Poor Environments. Microorganisms 2021, 9, 250. https://doi.org/10.3390/microorganisms9020250

AMA Style

Lalaouna D, Fochesato S, Harir M, Ortet P, Schmitt-Kopplin P, Heulin T, Achouak W. Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival of Pseudomonas brassicacerum in Nutrient-Poor Environments. Microorganisms. 2021; 9(2):250. https://doi.org/10.3390/microorganisms9020250

Chicago/Turabian Style

Lalaouna, David, Sylvain Fochesato, Mourad Harir, Philippe Ortet, Philippe Schmitt-Kopplin, Thierry Heulin, and Wafa Achouak. 2021. "Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival of Pseudomonas brassicacerum in Nutrient-Poor Environments" Microorganisms 9, no. 2: 250. https://doi.org/10.3390/microorganisms9020250

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