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Article

In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis

1
Department of Ophthalmology and Visual Science, Kochi Medical School, Kochi University, Kochi 783-8505, Japan
2
Laboratory of Veterinary Microbiology I, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan
3
Science Research Center, Kochi University, Kochi 783-8505, Japan
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Department of Ophthalmology, Toho University, Tokyo 143-8541, Japan
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Department of Ophthalmology, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan
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Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kochi 783-8505, Japan
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Department of Medical Laboratory Science, Faculty of Health Sciences, Kochi Gakuen University, Kochi 780-0955, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Marco Maria D’Andrea
Microorganisms 2021, 9(2), 212; https://doi.org/10.3390/microorganisms9020212
Received: 29 December 2020 / Revised: 14 January 2021 / Accepted: 17 January 2021 / Published: 20 January 2021
Post-operative endophthalmitis caused by Enterococcus spp. progresses rapidly and often results in substantial and irreversible vision loss. Therefore, novel alternative treatments that are effective against enterococcal endophthalmitis are required. Bacteriophage therapy has the potential to be an optional therapy for infectious diseases. Therefore, we investigated the therapeutic potential of three newly isolated enterococcal phages, phiEF7H, phiEF14H1, and phiEF19G, in E. faecalis-induced endophthalmitis. These phages could lyse the broad-range E. faecalis, including strains derived from endophthalmitis and vancomycin-resistant E. faecalis in vitro, as determined by the streak test. Morphological and genomic analyses revealed that these phages were classified into the Herelleviridae genus Kochikohdavirus. The whole genomes of these phages contained 143,399, 143,280, and 143,400 bp, respectively. Endophthalmitis was induced in mice by injection of three strains of E. faecalis derived from post-operative endophthalmitis or vancomycin-resistant strains into the vitreous body. The number of viable bacteria and infiltration of neutrophils in the eye were both decreased by intravitreous injection of phiEF7H, phiEF14H1, and phiEF19G 6 h after injection of all E. faecalis strains. Thus, these results suggest that these newly isolated phages may serve as promising candidates for phage therapy against endophthalmitis. View Full-Text
Keywords: Enterococcus faecalis; bacteriophage; endophthalmitis; Herelleviridae; mouse model; phage therapy Enterococcus faecalis; bacteriophage; endophthalmitis; Herelleviridae; mouse model; phage therapy
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MDPI and ACS Style

Kishimoto, T.; Ishida, W.; Nasukawa, T.; Ujihara, T.; Nakajima, I.; Suzuki, T.; Uchiyama, J.; Todokoro, D.; Daibata, M.; Fukushima, A.; Matsuzaki, S.; Fukuda, K. In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis. Microorganisms 2021, 9, 212. https://doi.org/10.3390/microorganisms9020212

AMA Style

Kishimoto T, Ishida W, Nasukawa T, Ujihara T, Nakajima I, Suzuki T, Uchiyama J, Todokoro D, Daibata M, Fukushima A, Matsuzaki S, Fukuda K. In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis. Microorganisms. 2021; 9(2):212. https://doi.org/10.3390/microorganisms9020212

Chicago/Turabian Style

Kishimoto, Tatsuma, Waka Ishida, Tadahiro Nasukawa, Takako Ujihara, Isana Nakajima, Takashi Suzuki, Jumpei Uchiyama, Daisuke Todokoro, Masanori Daibata, Atsuki Fukushima, Shigenobu Matsuzaki, and Ken Fukuda. 2021. "In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis" Microorganisms 9, no. 2: 212. https://doi.org/10.3390/microorganisms9020212

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