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Article

Bap-Independent Biofilm Formation in Staphylococcus xylosus

1
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, 85354 Freising, Germany
2
Bayerisches Zentrum für Biomolekulare Massenspektrometrie (BayBioMS), 85354 Freising, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Edward Fox
Microorganisms 2021, 9(12), 2610; https://doi.org/10.3390/microorganisms9122610
Received: 30 November 2021 / Revised: 14 December 2021 / Accepted: 15 December 2021 / Published: 17 December 2021
(This article belongs to the Section Biofilm)
The biofilm associated protein (Bap) is recognised as the essential component for biofilm formation in Staphylococcus aureus V329 and has been predicted as important for other species as well. Although Bap orthologs are also present in most S. xylosus strains, their contribution to biofilm formation has not yet been demonstrated. In this study, different experimental approaches were used to elucidate the effect of Bap on biofilm formation in S. xylosus and the motif structure of two biofilm-forming S. xylosus strains TMW 2.1023 and TMW 2.1523 was compared to Bap of S. aureus V329. We found that despite an identical structural arrangement into four regions, Bap from S. xylosus differs in key factors to Bap of S. aureus, i.e., isoelectric point of aggregation prone Region B, protein homology and type of repeats. Disruption of bap had no effect on aggregation behavior of selected S. xylosus strains and biofilm formation was unaffected (TMW 2.1023) or at best slightly reduced under neutral conditions (TMW 2.1523). Further, we could not observe any typical characteristics of a S. aureus Bap-positive phenotype such as functional impairment by calcium addition and rough colony morphology on congo red agar (CRA). A dominating role of Bap in cell aggregation and biofilm formation as reported mainly for S. aureus V329 was not observed. In contrast, this work demonstrates that functions of S. aureus Bap cannot easily be extrapolated to S. xylosus Bap, which appears as non-essential for biofilm formation in this species. We therefore suggest that biofilm formation in S. xylosus follows different and multifactorial mechanisms. View Full-Text
Keywords: Staphylococcus xylosus; knockout; Bap; biofilm; aggregation Staphylococcus xylosus; knockout; Bap; biofilm; aggregation
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MDPI and ACS Style

Schiffer, C.J.; Abele, M.; Ehrmann, M.A.; Vogel, R.F. Bap-Independent Biofilm Formation in Staphylococcus xylosus. Microorganisms 2021, 9, 2610. https://doi.org/10.3390/microorganisms9122610

AMA Style

Schiffer CJ, Abele M, Ehrmann MA, Vogel RF. Bap-Independent Biofilm Formation in Staphylococcus xylosus. Microorganisms. 2021; 9(12):2610. https://doi.org/10.3390/microorganisms9122610

Chicago/Turabian Style

Schiffer, Carolin J., Miriam Abele, Matthias A. Ehrmann, and Rudi F. Vogel. 2021. "Bap-Independent Biofilm Formation in Staphylococcus xylosus" Microorganisms 9, no. 12: 2610. https://doi.org/10.3390/microorganisms9122610

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