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Microorganisms
Volume 9
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10.3390/microorganisms9122490
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Article

Identification of an Exopolysaccharide Biosynthesis Gene in Bradyrhizobium diazoefficiens USDA110

by
Chunxia Xu
1,2,†,
Huaqin Ruan
3,†,
Wenjie Cai
1,2,†,
Christian Staehelin
3 and
Weijun Dai
1,2,*
1
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510006, China
2
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Center, South China Agricultural University, Guangzhou 510642, China
3
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Bioresources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2021, 9(12), 2490; https://doi.org/10.3390/microorganisms9122490
Submission received: 26 October 2021 / Revised: 24 November 2021 / Accepted: 25 November 2021 / Published: 1 December 2021
(This article belongs to the Special Issue Genomics of Nitrogen-Fixing Plant Symbiotic Bacteria)
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Abstract

Exopolysaccharides (EPS) play critical roles in rhizobium-plant interactions. However, the EPS biosynthesis pathway in Bradyrhizobium diazoefficiens USDA110 remains elusive. Here we used transposon (Tn) mutagenesis with the aim to identify genetic elements required for EPS biosynthesis in B. diazoefficiens USDA110. Phenotypic screening of Tn5 insertion mutants grown on agar plates led to the identification of a mutant with a transposon insertion site in the blr2358 gene. This gene is predicted to encode a phosphor-glycosyltransferase that transfers a phosphosugar onto a polyprenol phosphate substrate. The disruption of the blr2358 gene resulted in defective EPS synthesis. Accordingly, the blr2358 mutant showed a reduced capacity to induce nodules and stimulate the growth of soybean plants. Glycosyltransferase genes related to blr2358 were found to be well conserved and widely distributed among strains of the Bradyrhizobium genus. In conclusion, our study resulted in identification of a gene involved in EPS biosynthesis and highlights the importance of EPS in the symbiotic interaction between USDA110 and soybeans.
Keywords: exopolysaccharide; Bradyrhizobium; plant growth promotion exopolysaccharide; Bradyrhizobium; plant growth promotion

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MDPI and ACS Style

Xu, C.; Ruan, H.; Cai, W.; Staehelin, C.; Dai, W. Identification of an Exopolysaccharide Biosynthesis Gene in Bradyrhizobium diazoefficiens USDA110. Microorganisms 2021, 9, 2490. https://doi.org/10.3390/microorganisms9122490

AMA Style

Xu C, Ruan H, Cai W, Staehelin C, Dai W. Identification of an Exopolysaccharide Biosynthesis Gene in Bradyrhizobium diazoefficiens USDA110. Microorganisms. 2021; 9(12):2490. https://doi.org/10.3390/microorganisms9122490

Chicago/Turabian Style

Xu, Chunxia, Huaqin Ruan, Wenjie Cai, Christian Staehelin, and Weijun Dai. 2021. "Identification of an Exopolysaccharide Biosynthesis Gene in Bradyrhizobium diazoefficiens USDA110" Microorganisms 9, no. 12: 2490. https://doi.org/10.3390/microorganisms9122490

APA Style

Xu, C., Ruan, H., Cai, W., Staehelin, C., & Dai, W. (2021). Identification of an Exopolysaccharide Biosynthesis Gene in Bradyrhizobium diazoefficiens USDA110. Microorganisms, 9(12), 2490. https://doi.org/10.3390/microorganisms9122490

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