Regulation of d-Aspartate Oxidase Gene Expression by Pyruvate Metabolism in the Yeast Cryptococcus humicola
Round 1
Reviewer 1 Report
This manuscript describes the role of pyruvate carboxylase (PYC) in regulation of D-aspartate oxidase (DDO) gene expression. PYC was found to modulate DDO gene expression through metabolism of pyruvate to oxaloacetate, with pyruvate acting as a negative regulator of DDO expression and oxaloacetate exerting positive regulation.
This study was thorough and the conclusions are sound. The manuscript is well-written.
Two (very) minor issues:
- In Figure 2, the p-value should be indicated by ** for consistency with other figures.
- In Figure 6, it is unnecessary to go beyond the standard **** for p-value<0.0005.
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The manuscript reports a complete study on the regulation of DDO gene in the yeast Cryptococcus humicola by pyruvate metabolism.
POINT 1: I reccomend the following title "Regulation of D-Aspartate oxidase gene expression by pyruvate metabolism in the yeast Cryptococcus humicola"
POINT 2: Check block letters in "D-Aspartate oxidase" (Lines 2, 8, 22, 26 )
Author Response
We thank the reviewer for the careful and critical reading. We are glad to hear the positive comments of the reviewer. The valuable comments and suggestions described below have helped us to improve our manuscript significantly. According to the reviewer’s comments, we have revised the manuscript appropriately.
POINT 1: I reccomend the following title "Regulation of D-Aspartate oxidase gene expression by pyruvate metabolism in the yeast Cryptococcus humicola"
According to the reviewer’s suggestion, we have changed the previous title with the proposed title “Regulation of d-Aspartate Oxidase Gene Expression by Pyruvate Metabolism in the Yeast Cryptococcus humicola.”
POINT 2: Check block letters in "D-Aspartate oxidase" (Lines 2, 8, 22, 26 )
We thank the reviewer for the suggestion. We have checked the block letters in the “d-Aspartate oxidase.” We have used the word "d-Aspartate oxidase" in the title and at the beginning of a sentence and "d-aspartate oxidase" at the middle of a sentence and in the keywords, according to the journal’s submission guidelines.
Another correction:
In Figure 7, we have changed the English error "Nuclear" to "Nucleus."
