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Article
Peer-Review Record

Regulation of d-Aspartate Oxidase Gene Expression by Pyruvate Metabolism in the Yeast Cryptococcus humicola

Microorganisms 2021, 9(12), 2444; https://doi.org/10.3390/microorganisms9122444
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Microorganisms 2021, 9(12), 2444; https://doi.org/10.3390/microorganisms9122444
Received: 10 November 2021 / Revised: 24 November 2021 / Accepted: 25 November 2021 / Published: 27 November 2021
(This article belongs to the Section Molecular Microbiology and Immunology)

Round 1

Reviewer 1 Report

This manuscript describes the role of pyruvate carboxylase (PYC) in regulation of D-aspartate oxidase (DDO) gene expression. PYC was found to modulate DDO gene expression through metabolism of pyruvate to oxaloacetate, with pyruvate acting as a negative regulator of DDO expression and oxaloacetate exerting positive regulation.

This study was thorough and the conclusions are sound. The manuscript is well-written.

Two (very) minor issues:

  1. In Figure 2, the p-value should be indicated by ** for consistency with other figures.
  2. In Figure 6, it is unnecessary to go beyond the standard **** for p-value<0.0005.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

The manuscript reports a complete study on the regulation of DDO gene in the yeast Cryptococcus humicola by pyruvate metabolism. 

POINT 1:  I reccomend the following title "Regulation of D-Aspartate oxidase gene expression by pyruvate metabolism in the yeast Cryptococcus humicola"

POINT 2: Check block letters in "D-Aspartate oxidase" (Lines 2, 8, 22, 26 )

 

 

Author Response

We thank the reviewer for the careful and critical reading. We are glad to hear the positive comments of the reviewer. The valuable comments and suggestions described below have helped us to improve our manuscript significantly. According to the reviewer’s comments, we have revised the manuscript appropriately.

POINT 1:  I reccomend the following title "Regulation of D-Aspartate oxidase gene expression by pyruvate metabolism in the yeast Cryptococcus humicola"

According to the reviewer’s suggestion, we have changed the previous title with the proposed title “Regulation of d-Aspartate Oxidase Gene Expression by Pyruvate Metabolism in the Yeast Cryptococcus humicola.”

POINT 2: Check block letters in "D-Aspartate oxidase" (Lines 2, 8, 22, 26 )

We thank the reviewer for the suggestion. We have checked the block letters in the “d-Aspartate oxidase.” We have used the word "d-Aspartate oxidase" in the title and at the beginning of a sentence and "d-aspartate oxidase" at the middle of a sentence and in the keywords, according to the journal’s submission guidelines.

Another correction:

In Figure 7, we have changed the English error "Nuclear" to "Nucleus."

 

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