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Article

Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection

1
Laboratorio de Fitoquímica, Posgrado de Botánica, Colegio de Postgraduados, Texcoco 56230, Mexico
2
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico
3
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico
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Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, Mexico
5
Departamento de Productos Naturales, Instituto de Química, Universidad Nacional Autόnoma de México, Ciudad de México 04510, Mexico
*
Authors to whom correspondence should be addressed.
These authors contributed equally.
Academic Editor: Ronit Sionov
Microorganisms 2021, 9(12), 2424; https://doi.org/10.3390/microorganisms9122424
Received: 2 October 2021 / Revised: 13 November 2021 / Accepted: 15 November 2021 / Published: 25 November 2021
Several plant extracts exhibit anti-virulence properties due to the interruption of bacterial quorum sensing (QS). However, studies on their effects at the preclinical level are scarce. Here, we used a murine model of abscess/necrosis induced by Pseudomonas aeruginosa to evaluate the anti-pathogenic efficacy of 24 plant extracts at a sub-inhibitory concentration. We analyzed their ability to inhibit QS-regulated virulence factors such as swarming, pyocyanin production, and secretion of the ExoU toxin via the type III secretion system (T3SS). Five of the seven extracts with the best anti-pathogenic activity reduced ExoU secretion, and the extracts of Diphysa americana and Hibiscus sabdariffa were identified as the most active. Therefore, the abscess/necrosis model allows identification of plant extracts that have the capacity to reduce pathogenicity of P. aeruginosa. Furthermore, we evaluated the activity of the plant extracts on Chromobacterium violaceum. T3SS (ΔescU) and QS (ΔcviI) mutant strains were assessed in both the abscess/necrosis and sepsis models. Only the ΔescU strain had lower pathogenicity in the animal models, although no activity of plant extracts was observed. These results demonstrate differences between the anti-virulence activity recorded in vitro and pathogenicity in vivo and between the roles of QS and T3S systems as virulence determinants. View Full-Text
Keywords: cutaneous infection model; type III secretion system; quorum sensing; Chromobacterium violaceum; Pseudomonas aeruginosa; Diphysa americana; Hibiscus sabdariffa cutaneous infection model; type III secretion system; quorum sensing; Chromobacterium violaceum; Pseudomonas aeruginosa; Diphysa americana; Hibiscus sabdariffa
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MDPI and ACS Style

Díaz-Núñez, J.L.; Pérez-López, M.; Espinosa, N.; Campos-Hernández, N.; García-Contreras, R.; Díaz-Guerrero, M.; Cortes-López, H.; Vázquez-Sánchez, M.; Quezada, H.; Martínez-Vázquez, M.; Soto-Hernández, R.M.; Burgos-Hernández, M.; González-Pedrajo, B.; Castillo-Juárez, I. Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection. Microorganisms 2021, 9, 2424. https://doi.org/10.3390/microorganisms9122424

AMA Style

Díaz-Núñez JL, Pérez-López M, Espinosa N, Campos-Hernández N, García-Contreras R, Díaz-Guerrero M, Cortes-López H, Vázquez-Sánchez M, Quezada H, Martínez-Vázquez M, Soto-Hernández RM, Burgos-Hernández M, González-Pedrajo B, Castillo-Juárez I. Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection. Microorganisms. 2021; 9(12):2424. https://doi.org/10.3390/microorganisms9122424

Chicago/Turabian Style

Díaz-Núñez, José L., Macrina Pérez-López, Norma Espinosa, Nayelli Campos-Hernández, Rodolfo García-Contreras, Miguel Díaz-Guerrero, Humberto Cortes-López, Monserrat Vázquez-Sánchez, Héctor Quezada, Mariano Martínez-Vázquez, Ramón M. Soto-Hernández, Mireya Burgos-Hernández, Bertha González-Pedrajo, and Israel Castillo-Juárez. 2021. "Anti-Virulence Properties of Plant Species: Correlation between In Vitro Activity and Efficacy in a Murine Model of Bacterial Infection" Microorganisms 9, no. 12: 2424. https://doi.org/10.3390/microorganisms9122424

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