Characterization of “Candidatus Ehrlichia Pampeana” in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Targeted Gene | Sequence (5′–3′) | Amplicon Size (bp) | Reference |
---|---|---|---|---|
EHR16SD * EHR16SR * fD1 | 16S rRNA | GGT ACC YAC AGA AGA AGT CC | 345 | [21] |
TAG CAC TCA TCG TTT ACA GC | [21] | |||
AGA GTT TGA TCC TGG CTC AG | ~1431 | [22,23] | ||
rP2 | ACG GCT ACC TTG TTA CGA CTT | [22,23] | ||
HS1a | groEL | AIT GGG CTG GTA ITG AAA T | ~1400 | [24,26] |
HS6a | CCI CCI GGI ACI AIA CCT TC | [24,26] | ||
HS43 | ATW GCW AAR GAA GCA TAG TC | 1297 | [25] | |
HSVR | CTC AAC AGC AGC TCT AGT AGC | [25] | ||
Dsb-330 | dsb | GAT GAT GTT TGA AGA TAT SAA ACA AAT | 401 | [27,28] |
Dsb-720 ** Dsb-380 | CTA TTT TAC TTC TTA AAG TTG ATA WAT C | 349 | [27,28] | |
ATT TTT AGR GAT TTT CCA ATA CTT GG | [28] |
Collection Site | Stage | N° of Ticks Processed | Pools | Positive Pools | Positive Pools Code | GenBank Accession Numbers | ||
---|---|---|---|---|---|---|---|---|
16S rRNA | dsb | groEL | ||||||
Gruta de los Cuervos (T) | Female | 65 | 7 | 1 | S16HH13 | MZ733618 | MZ779092 | MZ779098 |
Male | 77 | 8 | 1 | S12HM25 | - | MZ779089 | - | |
Nymph | 969 | 29 | 2 | S11HN18 S14HN39 | MZ733620 MZ733619 | MZ779088 MZ779091 | MZ779097 MZ779095 | |
Amarillo (Ri) | Female | 0 | 0 | 0 | ||||
Male | 0 | 0 | 0 | |||||
Nymph | 12 | 2 | 0 | |||||
Lunarejo (Ri) | Female | 10 | 3 | 0 | ||||
Male | 15 | 4 | 0 | |||||
Nymph | 401 | 15 | 1 | S14HN30 | - | MZ779090 | MZ779094 | |
Reserva Natural Salus (L) | Female | 3 | 2 | 0 | ||||
Male | 4 | 1 | 0 | |||||
Nymph | 124 | 7 | 0 | |||||
Laguna Negra (Ro) | Female | 7 | 2 | 0 | ||||
Male | 2 | 1 | 0 | |||||
Nymph | 175 | 18 | 1 | S8HN5 | MZ733617 | MZ779093 | - | |
Total | 1864 | 99 | 6 |
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Félix, M.L.; Muñoz-Leal, S.; Carvalho, L.A.; Queirolo, D.; Remesar, S.; Armúa-Fernández, M.T.; Venzal, J.M. Characterization of “Candidatus Ehrlichia Pampeana” in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay. Microorganisms 2021, 9, 2165. https://doi.org/10.3390/microorganisms9102165
Félix ML, Muñoz-Leal S, Carvalho LA, Queirolo D, Remesar S, Armúa-Fernández MT, Venzal JM. Characterization of “Candidatus Ehrlichia Pampeana” in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay. Microorganisms. 2021; 9(10):2165. https://doi.org/10.3390/microorganisms9102165
Chicago/Turabian StyleFélix, María Laura, Sebastián Muñoz-Leal, Luis Andrés Carvalho, Diego Queirolo, Susana Remesar, María Teresa Armúa-Fernández, and José Manuel Venzal. 2021. "Characterization of “Candidatus Ehrlichia Pampeana” in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay" Microorganisms 9, no. 10: 2165. https://doi.org/10.3390/microorganisms9102165