Next Article in Journal
Phylogenetic Identification, Diversity, and Richness of Aspergillus from Homes in Havana, Cuba
Next Article in Special Issue
Bacteria Broadly-Resistant to Last Resort Antibiotics Detected in Commercial Chicken Farms
Previous Article in Journal
Metagenomics and Culture Dependent Insights into the Distribution of Firmicutes across Two Different Sample Types Located in the Black Hills Region of South Dakota, USA
Previous Article in Special Issue
Detection of Oxazolidinone Resistance Genes and Characterization of Genetic Environments in Enterococci of Swine Origin, Italy
Article

Unearthing Antibiotic Resistance Associated with Disturbance-Induced Permafrost Thaw in Interior Alaska

1
Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK 99775, USA
2
Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK 99775, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2021, 9(1), 116; https://doi.org/10.3390/microorganisms9010116
Received: 17 December 2020 / Revised: 31 December 2020 / Accepted: 31 December 2020 / Published: 6 January 2021
(This article belongs to the Special Issue Antimicrobial Resistance: From the Environment to Human Health)
Monitoring antibiotic resistance genes (ARGs) across ecological niches is critical for assessing the impacts distinct microbial communities have on the global spread of resistance. In permafrost-associated soils, climate and human driven disturbances augment near-surface thaw shifting the predominant bacteria that shape the resistome in overlying active layer soils. This thaw is of concern in Alaska, because 85% of land is underlain by permafrost, making soils especially vulnerable to disturbances. The goal of this study is to assess how soil disturbance, and the subsequent shift in community composition, will affect the types, abundance, and mobility of ARGs that compose the active layer resistome. We address this goal through the following aims: (1) assess resistance phenotypes through antibiotic susceptibility testing, and (2) analyze types, abundance, and mobility of ARGs through whole genome analyses of bacteria isolated from a disturbance-induced thaw gradient in Interior Alaska. We found a high proportion of isolates resistant to at least one of the antibiotics tested with the highest prevalence of resistance to ampicillin. The abundance of ARGs and proportion of resistant isolates increased with disturbance; however, the number of ARGs per isolate was explained more by phylogeny than isolation site. When compared to a global database of soil bacteria, RefSoil+, our isolates from the same genera had distinct ARGs with a higher proportion on plasmids. These results emphasize the hypothesis that both phylogeny and ecology shape the resistome and suggest that a shift in community composition as a result of disturbance-induced thaw will be reflected in the predominant ARGs comprising the active layer resistome. View Full-Text
Keywords: antibiotic resistance; environmental resistome; RefSoil+; soil disturbance; permafrost thaw antibiotic resistance; environmental resistome; RefSoil+; soil disturbance; permafrost thaw
Show Figures

Figure 1

MDPI and ACS Style

Haan, T.J.; Drown, D.M. Unearthing Antibiotic Resistance Associated with Disturbance-Induced Permafrost Thaw in Interior Alaska. Microorganisms 2021, 9, 116. https://doi.org/10.3390/microorganisms9010116

AMA Style

Haan TJ, Drown DM. Unearthing Antibiotic Resistance Associated with Disturbance-Induced Permafrost Thaw in Interior Alaska. Microorganisms. 2021; 9(1):116. https://doi.org/10.3390/microorganisms9010116

Chicago/Turabian Style

Haan, Tracie J., and Devin M. Drown 2021. "Unearthing Antibiotic Resistance Associated with Disturbance-Induced Permafrost Thaw in Interior Alaska" Microorganisms 9, no. 1: 116. https://doi.org/10.3390/microorganisms9010116

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop