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Open AccessArticle

Development of Monoclonal Antibodies and Antigen-Capture ELISA for Human Parechovirus Type 3

1
Department of Microbiology, Yokohama City University School of Medicine, Kanagawa 236-0004, Japan
2
Ibaraki Prefectural Institute of Public Health, Ibaraki 310-0852, Japan
3
Life Science Laboratory, Technology and Development Division, Kanto Chemical Co., Inc., Kanagawa 259-1146, Japan
4
Department of Health Science, Gunma Paz University Graduate School, Gunma 370-0006, Japan
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(9), 1437; https://doi.org/10.3390/microorganisms8091437
Received: 25 August 2020 / Revised: 13 September 2020 / Accepted: 17 September 2020 / Published: 19 September 2020
Human parechovirus type 3 (HPeV3) is an etiologic agent of respiratory diseases, meningitis, and sepsis-like illness in both infants and adults. Monoclonal antibodies (mAbs) can be a promising diagnostic tool for antigenic diseases such as virus infection, as they offer a high specificity toward a specific viral antigen. However, to date, there is no specific mAb available for the diagnosis of HPeV3 infection. In this study, we developed and characterized mAbs specific for HPeV3 capsid protein VP0. We used cell-free, wheat germ-synthesized viral VP0 protein for immunizing BALB/c mice to generate hybridomas. From the resultant hybridoma clones, we selected nine clones producing mAbs reactive to the HPeV3-VP0 antigen, based on enzyme-linked immunosorbent assay (ELISA). Epitope mapping showed that these mAbs recognized three distinct domains in HPeV3 VP0. Six mAbs recognized HPeV3 specifically and the other three mAbs showed cross-reactivity with other HPeVs. Using the HPeV3-specific mAbs, we then developed an ELISA for viral antigen detection that could be reliably used for laboratory diagnosis of HPeV3. This ELISA system exhibited no cross-reactivity with other related viruses. Our newly developed mAbs would, thus, provide a useful set of tools for future research and ensure HPeV3-specific diagnosis. View Full-Text
Keywords: human parechovirus; monoclonal antibodies; ELISA; VP0 human parechovirus; monoclonal antibodies; ELISA; VP0
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MDPI and ACS Style

Goto, K.; Yamaoka, Y.; Khatun, H.; Miyakawa, K.; Nishi, M.; Nagata, N.; Yanaoka, T.; Kimura, H.; Ryo, A. Development of Monoclonal Antibodies and Antigen-Capture ELISA for Human Parechovirus Type 3. Microorganisms 2020, 8, 1437. https://doi.org/10.3390/microorganisms8091437

AMA Style

Goto K, Yamaoka Y, Khatun H, Miyakawa K, Nishi M, Nagata N, Yanaoka T, Kimura H, Ryo A. Development of Monoclonal Antibodies and Antigen-Capture ELISA for Human Parechovirus Type 3. Microorganisms. 2020; 8(9):1437. https://doi.org/10.3390/microorganisms8091437

Chicago/Turabian Style

Goto, Keiko; Yamaoka, Yutaro; Khatun, Hajera; Miyakawa, Kei; Nishi, Mayuko; Nagata, Noriko; Yanaoka, Toshikazu; Kimura, Hirokazu; Ryo, Akihide. 2020. "Development of Monoclonal Antibodies and Antigen-Capture ELISA for Human Parechovirus Type 3" Microorganisms 8, no. 9: 1437. https://doi.org/10.3390/microorganisms8091437

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