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Article

Fungistatic Action of N-Acetylcysteine on Candida albicans Biofilms and Its Interaction with Antifungal Agents

Laboratory of Applied Microbiology, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, SP, Brazil
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Microorganisms 2020, 8(7), 980; https://doi.org/10.3390/microorganisms8070980
Received: 10 June 2020 / Revised: 23 June 2020 / Accepted: 23 June 2020 / Published: 30 June 2020
(This article belongs to the Special Issue Candida albicans Virulence Factors and Its Pathogenecity)
Therapies targeted to fungal biofilms, mainly against the matrix, and therapies that do not induce microbial resistance are relevant. N-acetylcysteine (NAC), a mucolytic agent, has shown antimicrobial action. This study evaluated the effect of NAC against fluconazole-susceptible (CaS) and -resistant (CaR) Candida albicans. The susceptibility of planktonic cultures to NAC, the effect of NAC on biofilms and their matrix, the interaction of NAC with antifungal agents, and confocal microscopy were evaluated. Data were analyzed descriptively and by the ANOVA/Welch and Tukey/Gomes–Howell tests. The minimum inhibitory concentration (MIC) of NAC was 25 mg/mL for both strains. NAC significantly reduced the viability of both fungal strains. Concentrations higher than the MIC (100 and 50 mg/mL) reduced the viability and the biomass. NAC at 12.5 mg/mL increased the fungal viability. NAC also reduced the soluble components of the biofilm matrix, and showed synergism with caspofungin against planktonic cultures of CaS, but not against biofilms. Confocal images demonstrated that NAC reduced the biofilm thickness and the fluorescence intensity of most fluorochromes used. High concentrations of NAC had similar fungistatic effects against both strains, while a low concentration showed the opposite result. The antibiofilm action of NAC was due to its fungistatic action. View Full-Text
Keywords: acetylcysteine; biofilms; extracellular matrix; Candida albicans; drug resistance; antifungal agents acetylcysteine; biofilms; extracellular matrix; Candida albicans; drug resistance; antifungal agents
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MDPI and ACS Style

Nunes, T.S.B.S.; Rosa, L.M.; Vega-Chacón, Y.; Mima, E.G.d.O. Fungistatic Action of N-Acetylcysteine on Candida albicans Biofilms and Its Interaction with Antifungal Agents. Microorganisms 2020, 8, 980. https://doi.org/10.3390/microorganisms8070980

AMA Style

Nunes TSBS, Rosa LM, Vega-Chacón Y, Mima EGdO. Fungistatic Action of N-Acetylcysteine on Candida albicans Biofilms and Its Interaction with Antifungal Agents. Microorganisms. 2020; 8(7):980. https://doi.org/10.3390/microorganisms8070980

Chicago/Turabian Style

Nunes, Thaís S.B.S., Leticia M. Rosa, Yuliana Vega-Chacón, and Ewerton G.d.O. Mima. 2020. "Fungistatic Action of N-Acetylcysteine on Candida albicans Biofilms and Its Interaction with Antifungal Agents" Microorganisms 8, no. 7: 980. https://doi.org/10.3390/microorganisms8070980

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