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Article

Maturational Changes Alter Effects of Dietary Phytase Supplementation on the Fecal Microbiome in Fattening Pigs

1
Unit Nutritional Physiology, Institute of Physiology, Pathophysiology and Biophysics, Department of Biomedical Sciences, University of Veterinary Medicine, 1210 Vienna, Austria
2
Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, Austria
3
Institute of Food Research and Product Development, University of Kasetsart, Bangkok 10900, Thailand
4
University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, Austria
5
vetFarm, University of Veterinary Medicine, 1210 Vienna, Austria
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(7), 1073; https://doi.org/10.3390/microorganisms8071073
Received: 29 June 2020 / Revised: 14 July 2020 / Accepted: 16 July 2020 / Published: 18 July 2020
(This article belongs to the Special Issue Gut Microbiota Development in Farm Animals)
Age-related successions in the porcine gut microbiome may modify the microbial response to dietary changes. This may especially affect the bacterial response to essential nutrients for bacterial metabolism, such as phosphorus (P). Against this background, we used phytase supplementation (0 or 650 phytase units/kg complete feed) to alter the P availability in the hindgut and studied the dietary response of the fecal bacterial microbiome from the early to late fattening period. Fecal DNA were isolated after 0, 3, 5 and 10 weeks and the V3-V4 region of the 16S rRNA gene was sequenced. Permutational analysis of variance showed distinct bacterial communities for diet and week. Alpha-diversity and taxonomy indicated progressing maturation of the bacterial community with age. Prevotellaceae declined, whereas Clostridiaceae and Ruminococcaceae increased from weeks 0 to 3, 5, and 10, indicating changes in fiber-digesting capacities with age. Phytase affected all major bacterial taxa but reduced species richness (Chao1) and diversity (Shannon and Simpson). To conclude, present results greatly support the importance of available P for bacterial proliferation, including fibrolytic, lactic acid- and butyrate-producing genera, in pigs. Results also emphasize the necessity to assess bacterial responses to dietary manipulation at several time points throughout the fattening period. View Full-Text
Keywords: age; bacterial clusters; feed intake; feces; microbiome; pig; phytase age; bacterial clusters; feed intake; feces; microbiome; pig; phytase
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MDPI and ACS Style

Metzler-Zebeli, B.U.; Klinsoda, J.; Vötterl, J.C.; Verhovsek, D. Maturational Changes Alter Effects of Dietary Phytase Supplementation on the Fecal Microbiome in Fattening Pigs. Microorganisms 2020, 8, 1073. https://doi.org/10.3390/microorganisms8071073

AMA Style

Metzler-Zebeli BU, Klinsoda J, Vötterl JC, Verhovsek D. Maturational Changes Alter Effects of Dietary Phytase Supplementation on the Fecal Microbiome in Fattening Pigs. Microorganisms. 2020; 8(7):1073. https://doi.org/10.3390/microorganisms8071073

Chicago/Turabian Style

Metzler-Zebeli, Barbara U., Jutamat Klinsoda, Julia C. Vötterl, and Doris Verhovsek. 2020. "Maturational Changes Alter Effects of Dietary Phytase Supplementation on the Fecal Microbiome in Fattening Pigs" Microorganisms 8, no. 7: 1073. https://doi.org/10.3390/microorganisms8071073

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