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Article

Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea

by
Gulab Chand Arya
1,
Dhruv Aditya Srivastava
1,
Eswari P. J. Pandaranayaka
1,
Ekaterina Manasherova
1,
Dov Bernard Prusky
2,
Yigal Elad
3,
Omer Frenkel
3,
Hay Dvir
4 and
Arye Harel
1,*
1
Department of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, Israel
2
Department of Postharvest Science, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion7505101, Israel
3
Department of Plant Pathology and Weed Research, Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, Israel
4
Department of Ruminant Science, Institute of Animal Science, Agricultural Research Organization, Volcani Center, Rishon LeZion 7505101, Israel
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(7), 1043; https://doi.org/10.3390/microorganisms8071043
Submission received: 19 June 2020 / Revised: 10 July 2020 / Accepted: 11 July 2020 / Published: 14 July 2020
(This article belongs to the Special Issue Interplay between Fungal Pathogens and Harvested Crops and Fruits)
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Abstract

The necrotrophic fungus Botrytis cinerea, is considered a major cause of postharvest losses in a wide range of crops. The common fungal extracellular membrane protein (CFEM), containing a conserved eight-cysteine pattern, was found exclusively in fungi. Previous studies in phytopathogenic fungi have demonstrated the role of membrane-bound and secreted CFEM-containing proteins in different aspects of fungal virulence. However, non-G protein-coupled receptor (non-GPCR) membrane CFEM proteins have not been studied yet in phytopathogenic fungi. In the present study, we have identified a non-GPCR membrane-bound CFEM-containing protein, Bcin07g03260, in the B. cinerea genome, and generated deletion mutants, ΔCFEM-Bcin07g03260, to study its potential role in physiology and virulence. Three independent ΔCFEM-Bcin07g03260 mutants showed significantly reduced progression of a necrotic lesion on tomato (Solanum lycopersicum) leaves. Further analysis of the mutants revealed significant reduction (approximately 20–30%) in conidial germination and consequent germ tube elongation compared with the WT. Our data complements a previous study of secreted ΔCFEM1 mutants of B. cinerea that showed reduced progression of necrotic lesions on leaves, without effect on germination. Considering various functions identified for CFEM proteins in fungal virulence, our work illustrates a potential new role for a non-GPCR membrane CFEM in pathogenic fungi to control virulence in the fungus B. cinerea.
Keywords: Botrytis cinerea; virulence; CFEM; germination; pathogenicity Botrytis cinerea; virulence; CFEM; germination; pathogenicity

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MDPI and ACS Style

Arya, G.C.; Srivastava, D.A.; Pandaranayaka, E.P.J.; Manasherova, E.; Prusky, D.B.; Elad, Y.; Frenkel, O.; Dvir, H.; Harel, A. Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea. Microorganisms 2020, 8, 1043. https://doi.org/10.3390/microorganisms8071043

AMA Style

Arya GC, Srivastava DA, Pandaranayaka EPJ, Manasherova E, Prusky DB, Elad Y, Frenkel O, Dvir H, Harel A. Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea. Microorganisms. 2020; 8(7):1043. https://doi.org/10.3390/microorganisms8071043

Chicago/Turabian Style

Arya, Gulab Chand, Dhruv Aditya Srivastava, Eswari P. J. Pandaranayaka, Ekaterina Manasherova, Dov Bernard Prusky, Yigal Elad, Omer Frenkel, Hay Dvir, and Arye Harel. 2020. "Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea" Microorganisms 8, no. 7: 1043. https://doi.org/10.3390/microorganisms8071043

APA Style

Arya, G. C., Srivastava, D. A., Pandaranayaka, E. P. J., Manasherova, E., Prusky, D. B., Elad, Y., Frenkel, O., Dvir, H., & Harel, A. (2020). Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea. Microorganisms, 8(7), 1043. https://doi.org/10.3390/microorganisms8071043

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