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Article

Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt

1
Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, 07743 Jena, Germany
2
Institut for Animal Hygiene and Environmental Health, Free University of Berlin, 14163 Berlin, Germany
3
Department of Pathobiology, College of Veterinary and Animal Sciences, Jhang 35200, Pakistan
4
Department of Brucellosis, Animal Health Research Institute, Agricultural Research Center, Dokki 12618, Giza, Egypt
5
Veterinary Service Department, Armed Forces Logistics Authority, Egyptian Armed Forces, Nasr City 11765, Egypt
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Central Laboratory for Evaluation of Veterinary Biologics, Agricultural Research Center, Abbasaia 11517, Cairo, Egypt
7
Provincial Laboratory, Institute of Animal Health Research, Agricultural Research Center, Mansoura 35516, Egypt
8
Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Abbasaia 11517, Cairo, Egypt
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Climate Change Information Center, Renewable Energy and Expert Systems (CCICREES), Agricultural Research Center, 9 Algamaa Street, Giza 12619, Egypt
10
Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(7), 1035; https://doi.org/10.3390/microorganisms8071035
Received: 21 April 2020 / Revised: 8 July 2020 / Accepted: 9 July 2020 / Published: 13 July 2020
(This article belongs to the Special Issue Updates on Brucellosis)
Brucellosis is one of the most important worldwide zoonoses of many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study is focused on the three governorates with the highest camel populations and the largest camel markets in the country to determine the disease seroprevalence and identify the Brucella species in local camel holdings. In total, 381 serum samples were collected from male and female camels from Giza, Aswan, and Al-Bahr Al-Ahmar (the Red Sea) governorates. Samples were serologically examined using the Rose–Bengal plate test (RBPT), indirect ELISA (i-ELISA), competitive ELISA (c-ELISA) and complement fixation test (CFT). Brucella antibodies were detected in 59 (15.5%), 87 (22.8%), 77 (20.2%) and 118 (31.0%) of sera by RBPT, i-ELISA, c-ELISA and CFT, respectively. Using real-time PCR, Brucella DNA was amplified in 32 (8.4%) seropositive samples including Brucella abortus (25/32), Brucella suis (5/32) and Brucella melitensis (2/32), defining a complex epidemiological status. To the best of our knowledge, this is the first study reporting Brucella suis DNA in camel serum. The risk-associated factors including age, sex, breed and geographical distribution were statistically analyzed, showing non-significant association with seroprevalence. The results of this study will raise awareness for camel brucellosis and help develop effective control strategies. View Full-Text
Keywords: brucellosis; camel; B. suis; Egypt; seroprevalence; real-time PCR brucellosis; camel; B. suis; Egypt; seroprevalence; real-time PCR
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MDPI and ACS Style

Khan, A.U.; Sayour, A.E.; Melzer, F.; El-Soally, S.A.G.E.; Elschner, M.C.; Shell, W.S.; Moawad, A.A.; Mohamed, S.A.; Hendam, A.; Roesler, U.; Neubauer, H.; El-Adawy, H. Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt. Microorganisms 2020, 8, 1035. https://doi.org/10.3390/microorganisms8071035

AMA Style

Khan AU, Sayour AE, Melzer F, El-Soally SAGE, Elschner MC, Shell WS, Moawad AA, Mohamed SA, Hendam A, Roesler U, Neubauer H, El-Adawy H. Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt. Microorganisms. 2020; 8(7):1035. https://doi.org/10.3390/microorganisms8071035

Chicago/Turabian Style

Khan, Aman U., Ashraf E. Sayour, Falk Melzer, Sherif A.G.E. El-Soally, Mandy C. Elschner, Waleed S. Shell, Amira A. Moawad, Shereen A. Mohamed, Ashraf Hendam, Uwe Roesler, Heinrich Neubauer, and Hosny El-Adawy. 2020. "Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt" Microorganisms 8, no. 7: 1035. https://doi.org/10.3390/microorganisms8071035

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