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Article

Tetrahymena Glutathione Peroxidase Family: A Comparative Analysis of These Antioxidant Enzymes and Differential Gene Expression to Metals and Oxidizing Agents

1
ANSES (Laboratoire de Ploufragan-Plouzané-Niort, UVIPAC), 22440 Ploufragan, France
2
Department of Molecular Evolution, Centro de Astrobiología (CSIC-INTA), Carretera de Ajalvir km 4, Torrejón de Ardoz, 28850 Madrid, Spain
3
Departamento de Genética, Fisiología y Microbiología, Facultad de Biología. C/. José Antonio Nováis, 12. Universidad Complutense (UCM), 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(7), 1008; https://doi.org/10.3390/microorganisms8071008
Received: 30 May 2020 / Revised: 21 June 2020 / Accepted: 2 July 2020 / Published: 5 July 2020
(This article belongs to the Special Issue Microbial Stress Response to Toxic Metal(loid)s)
In the present work, an extensive analysis of the putative glutathione peroxidases (GPx) of the eukaryotic microorganism model Tetrahymena thermophila is carried out. A comparative analysis with GPx present in other Tetrahymena species and other very taxonomically diverse ciliates is also performed. A majority of ciliate GPx have replaced the selenocysteine (Sec) by Cys in its catalytic center, so they can be considered as phospholipid hydroperoxide glutathione peroxidases (PHGPx). Selenocysteine insertion sequence (SECIS) elements have been detected in several ciliate GPx that do not incorporate Sec in their amino acid sequences, and conversely, in other ciliate GPx with Sec, no SECIS elements are detected. These anomalies are analyzed and discussed. From the phylogenetic analysis using the ciliate GPx amino acid sequences, the existence of extensive intra- and interspecific gene duplications that produced multiple GPx isoforms in each species is inferred. The ancestral character of the selenoproteins is also corroborated. The analysis by qRT-PCR of six selected T. thermophila GPx genes has shown a quantitative differential expression between them, depending on the stressor (oxidizing agents, apoptotic inducer or metals) and the time of exposure. View Full-Text
Keywords: Glutathione peroxidases; selenocysteine; SECIS elements; gene expression; oxidative stress; Tetrahymena Glutathione peroxidases; selenocysteine; SECIS elements; gene expression; oxidative stress; Tetrahymena
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MDPI and ACS Style

Cubas-Gaona, L.L.; de Francisco, P.; Martín-González, A.; Gutiérrez, J.C. Tetrahymena Glutathione Peroxidase Family: A Comparative Analysis of These Antioxidant Enzymes and Differential Gene Expression to Metals and Oxidizing Agents. Microorganisms 2020, 8, 1008. https://doi.org/10.3390/microorganisms8071008

AMA Style

Cubas-Gaona LL, de Francisco P, Martín-González A, Gutiérrez JC. Tetrahymena Glutathione Peroxidase Family: A Comparative Analysis of These Antioxidant Enzymes and Differential Gene Expression to Metals and Oxidizing Agents. Microorganisms. 2020; 8(7):1008. https://doi.org/10.3390/microorganisms8071008

Chicago/Turabian Style

Cubas-Gaona, Liliana L., Patricia de Francisco, Ana Martín-González, and Juan C. Gutiérrez 2020. "Tetrahymena Glutathione Peroxidase Family: A Comparative Analysis of These Antioxidant Enzymes and Differential Gene Expression to Metals and Oxidizing Agents" Microorganisms 8, no. 7: 1008. https://doi.org/10.3390/microorganisms8071008

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